Transcriptomic data of human adrenocortical NCI-H295R cells treated with cortisol biosynthesis inhibitors.

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Despite the development of several classes of steroidogenesis inhibitors to treat human diseases associated with cortisol overproduction, their use is limited by insufficient efficacy, adverse effects, and/or tolerability. Recently, we identified a series of benzimidazolylurea derivatives, including the representative compound CJ28, as novel cortisol biosynthesis inhibitors [1]. They significantly inhibited both basal and stimulated production of cortisol in NCI-H295R cells, a human adrenocarcinoma cell line. The inhibitory effects were attributed to both attenuated steroidogenesis and de novo cholesterol biosynthesis. Here, we provide transcriptomic (RNA-seq) data from adrenal cell cultures in response to treatment with either CJ28 or metyrapone (MET), an inhibitor of 11β-hydroxylase). Total RNA was extracted from the cells treated with vehicle (0.1% DMSO), CJ28 (30 µM), or MET (30 µM) for 24 h. Primary sequence data were acquired using paired-end sequencing on an Illumina NovaSeq 6000 platform. The raw RNA-seq data have been deposited in the Gene Expression Omnibus (GEO) database (GSE236435). This dataset is a useful resource for providing valuable information on the gene expression networks underlying adrenocortical steroidogenesis.", "claims": [{"id": "claim-method", "statement": "Differential gene expression was assessed using normalized DESeq2 counts, and volcano plots were visualized using EnhancedVolcano [9].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38186738", "locator": "sections[10] \"Preparation of RNA samples and RNA-seq analysis\" paragraph 2", "quote": "Differential gene expression was assessed using normalized DESeq2 counts, and volcano plots were visualized using EnhancedVolcano [9]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Gene expression profiles of human adrenocortical NCI-H295R cells treated with cortisol biosynthesis inhibitors (Original data) (Gene expression omnibus)", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38186738", "locator": "sections[19] \"Data Availability\" paragraph 2", "quote": "Gene expression profiles of human adrenocortical NCI-H295R cells treated with cortisol biosynthesis inhibitors (Original data) (Gene expression omnibus)"}], "state": "complete"}, {"id": "claim-target-result", "statement": "Here, we provide transcriptomic (RNA-seq) data from adrenal cell cultures in response to treatment with either CJ28 or metyrapone (MET), an inhibitor of 11β-hydroxylase).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38186738", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Here, we provide transcriptomic (RNA-seq) data from adrenal cell cultures in response to treatment with either CJ28 or metyrapone (MET), an inhibitor of 11β-hydroxylase)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Here, we provide transcriptomic (RNA-seq) data from adrenal cell cultures in response to treatment with either CJ28 or metyrapone (MET), an inhibitor of 11β-hydroxylase).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38186738"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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