Differential Gene Expression and Immune Cell Infiltration in Patients with Steroid-induced Necrosis of the Femoral Head.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38204239", "kind": "science-record", "revision": 2, "content_hash": "sha256:59682f74e7da6bbc1459bfb7a75306d65f9e5f96401632c69983f34590c68163", "created_at": "2026-11-01T19:20:07Z", "updated_at": "2026-11-01T21:32:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Differential Gene Expression and Immune Cell Infiltration in Patients with Steroid-induced Necrosis of the Femoral Head.", "doi": "10.2174/0118715303266951231206114153", "pmid": "38204239", "authors": ["Guowu Ren", "Jie Han", "Jian Mo", "Zhiwei Xu", "Xinjian Feng", "Feng Chen", "Yukun Wu", "Qinglin Peng"], "journal": "Endocrine, metabolic & immune disorders drug targets", "year": 2024, "abstract": "OBJECTIVE: The study aimed to study the differential gene expression and immune cell infiltration in patients with steroid-induced necrosis of the femoral head (SANFH), identify the key genes and immune cells of SANFH, and explore the relationship between immune cells and SANFH.\nMETHODS: The high-throughput gene chip dataset GSE123568 was downloaded from the GEO database, and the differential gene expression was analyzed with the R language. The STRING database and Cytoscape software were used to analyze the protein interaction network and screen key genes, and enrichment analysis was carried out on key genes. The infiltration of immune cells in SANFH patients was analyzed and verified by immunohistochemistry.\nRESULTS: EP300, TRAF6, STAT1, JAK1, CASP8, and JAK2 are key genes in the pathogenesis of SANFH, which mainly involve myeloid cell differentiation, cytokine-mediated signaling pathway, tumor necrosis factor-mediated signaling pathway, and cellular response to tumor necrosis factor through JAK-STAT, NOD-like receptor, toll-like receptor, and other signaling pathways, leading to the occurrence of diseases; immune infiltration and immunohistochemical results have shown the expression of memory B cells and activated dendritic cells as reduced in SANFH patients, while in the same SANFH samples, M1 macrophages have been positively correlated with monocytes, and neutrophils have been negatively correlated with monocytes expression.\nCONCLUSION: EP300, TRAF6, STAT1, JAK1, CASP8, and JAK2 have exhibited significant differences in SANFH (spontaneous osteonecrosis of the femoral head). Memory B cells, activated dendritic cells, M1 macrophages, monocytes, and neutrophils have shown abnormal expression in SANFH.", "claims": [{"id": "claim-method", "statement": "A paired t-test was used to analyze the phenotypic differences between groups and estimate the relationship between each genotype and the risk of SANFH.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38204239", "locator": "sections[13] \"Statistical Analysis\" paragraph 2", "quote": "A paired t-test was used to analyze the phenotypic differences between groups and estimate the relationship between each genotype and the risk of SANFH."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data, used in this study can be obtained from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38204239", "locator": "sections[38] \"AVAILABILITY OF DATA AND MATERIALS\" paragraph 2", "quote": "The data, used in this study can be obtained from the corresponding author upon request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The results showed that compared to healthy controls, SANFH patients had a total of 1836 significantly altered genes, of which 1088 were up-regulated and 748 were down-regulated.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38204239", "locator": "sections[15] \"Analysis of SANFH Differential Genes\" paragraph 2", "quote": "The results showed that compared to healthy controls, SANFH patients had a total of 1836 significantly altered genes, of which 1088 were up-regulated and 748 were down-regulated."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The results showed that compared to healthy controls, SANFH patients had a total of 1836 significantly altered genes, of which 1088 were up-regulated and 748 were down-regulated.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38204239"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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