HPV16 E6 promoting cervical cancer progression through down-regulation of miR-320a to increase TOP2A expression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38205938", "kind": "science-record", "revision": 4, "content_hash": "sha256:b1f6f48d121c31645ae1225a7771b5456f271fb4b8f558e73d195162a48c2653", "created_at": "2026-11-01T19:20:07Z", "updated_at": "2026-11-01T22:53:26Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "HPV16 E6 promoting cervical cancer progression through down-regulation of miR-320a to increase TOP2A expression.", "doi": "10.1002/cam4.6875", "pmid": "38205938", "authors": ["Jianing Zhang", "Xiaohui Yu", "Yi Guo", "Daqing Wang"], "journal": "Cancer medicine", "year": 2024, "abstract": "BACKGROUND: Cervical cancer (CC) has become the fourth most common cancer worldwide and it is mainly caused by the infection of human papillomavirus (HPV), especially high-risk HPV16. Aberrant miRNA expression in CC is closely related to HPV16 infection, and the regulation of HPV16 E6 expression can affect a variety of miRNA expression. This study aims to exploring the miRNAs involved in E6 regulation in CC.\nMETHODS: Our study screened differentially expressed miRNAs in cervical cells of HPV16 infected and uninfected cervical cancer patients by analyzing the GSE81137 dataset of the gene expression omnibus database (GEO), and identified miR-320a that plays an anti-tumor role and is associated with good prognosis of cervical cancer. Explore the effect of HPV16 E6 on the expression of miR-320a in cervical cancer, and verify whether HPV16 E6 regulates the downstream target gene TOP2A expression through miR-320a, thereby affecting cervical cancer cell proliferation, apoptosis, migration, invasion, and EMT in vitro and in vivo.\nRESULTS: The bioinformatic methods selected the miR-320a, which was differentially expressed in cervical cells from HPV16-infected patients compared to uninfected patients. We further demonstrated that miR-320a level was regulated by HPV16 E6, which promoted the CC cell proliferation, migration, invasion, and inhibited apoptosis. In addition, we predicted the downstream target genes of miR-320a and confirmed that TOP2A was one of its targeting proteins. Moreover, HPV16 E6 promoted the TOP2A expression in CC cells through down-regulating miR-320a, leading to promoting CC development.\nCONCLUSIONS: We confirmed that HPV16 E6 promoted the TOP2A expression through down-regulation of miR-320a, thus promoting CC development, and the HPV16 E6/miR-320a/TOP2A axis may perform as a potential target for CC treatment.", "claims": [{"id": "claim-method", "statement": "Student's t‐test for independent samples was used for comparison between two groups. One‐way ANOVA was used for comparison between multiple groups, and Tukey post hoc test was used for comparison between two groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38205938", "locator": "sections[16] \"Statistical analysis\" paragraph 2", "quote": "Student's t‐test for independent samples was used for comparison between two groups. One‐way ANOVA was used for comparison between multiple groups, and Tukey post hoc test was used for comparison between two groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38205938", "locator": "sections[33] \"DATA AVAILABILITY STATEMENT\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We confirmed that HPV16 E6 promoted the TOP2A expression through down‐regulation of miR‐320a, thus promoting CC development, and the HPV16 E6/miR‐320a/TOP2A axis may perform as a potential target for CC treatment.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38205938", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "We confirmed that HPV16 E6 promoted the TOP2A expression through down‐regulation of miR‐320a, thus promoting CC development, and the HPV16 E6/miR‐320a/TOP2A axis may perform as a potential target for CC treatment."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "We confirmed that HPV16 E6 promoted the TOP2A expression through down‐regulation of miR‐320a, thus promoting CC development, and the HPV16 E6/miR‐320a/TOP2A axis may perform as a potential target for CC treatment.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38205938"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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C33a cells were transfected with miR‐320a mimics, and SiHa cells were tr", "ed with miR‐320a mimics, and SiHa cells were transfected with miR‐320a inhibitor according to the Lip"], "order_basis": "paper_text_order:3111", "id": "assay:4"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Student's t‐test for independent samples was used for comparison between two groups. One‐way ANOVA was used for comparison between multiple groups, and Tukey post hoc test was used for comparison between two groups."], "order_basis": "consumes_earlier_output", "id": "assay:5"}]}