Identification and Validation of a Prognostic Signature Derived from the Cancer Stem Cells for Oral Squamous Cell Carcinoma.

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In this study, data were obtained from a single-cell RNA-sequencing dataset (GSE172577) in the GEO database, and LASSO-Cox regression analysis was performed on 1344 CSCs-related genes to establish a six-gene prognostic signature (6-GPS) consisting of ADM, POLR1D, PTGR1, RPL35A, PGK1, and P4HA1. High-risk scores were significantly associated with unfavorable survival outcomes, and these features were thoroughly validated in the ICGC. The results of nomograms, calibration plots, and ROC curves confirmed the good prognostic accuracy of 6-GPS for OSCC. Additionally, the knockdown of ADM or POLR1D genes may significantly inhibit the proliferation, migration, and invasion of OSCC cells through the JAK/HIF-1 pathway. Furthermore, cell-cycle arrest occurred in the G1 phase by suppressing Cyclin D1. In summary, 6-GPS may play a crucial role in the occurrence and development of OSCC and has the potential to be developed further as a diagnostic, therapeutic, and prognostic tool for OSCC.", "claims": [{"id": "claim-method", "statement": "To mitigate overfitting, prognostic gene analysis was performed using the LASSO-Cox proportional hazards regression, utilizing the “survival” package [version 3.2-10] and the “glmnet” package [version 4.1-2].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38256104", "locator": "sections[15] \"4.3. 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Additionally, the knockdown of ADM or POLR1D genes may significantly inh", "Materials and Methods 4.8. Cell Culture and Transfection The OSCC cell lines CAL-27 (Servicebio, Wuha", "was seeded in T-25 cell culture flasks. For transfection, siRNAs targeting ADM (siRNA1-ADM, siRNA2-AD", "siRNA-NC) (GenePharma, Shanghai, China) were transfected into cells using the Lipofectamine™ 2000 tra"], "order_basis": "paper_text_order:822", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["n levels of ADM and POLR1D were assessed via RT-qPCR. 4.14. Statistical Analysis Statistical anal", "evels of ADM and POLR1D were assessed via RT-qPCR. 4.14. 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