Altered O-linked glycosylation in benign and malignant meningiomas.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38274327", "kind": "science-record", "revision": 2, "content_hash": "sha256:1cace33ba22403c4cf1a8a2200ba936be6933bf164be61e21b3a77a4f3c35445", "created_at": "2026-11-01T19:20:12Z", "updated_at": "2026-11-01T21:32:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Altered O-linked glycosylation in benign and malignant meningiomas.", "doi": "10.7717/peerj.16785", "pmid": "38274327", "authors": ["Chutima Talabnin", "Thanawat Trasaktaweesakul", "Pitchanun Jaturutthaweechot", "Pundit Asavaritikrai", "Dusit Kongnawakun", "Atit Silsirivanit", "Norie Araki", "Krajang Talabnin"], "journal": "PeerJ", "year": 2024, "abstract": "Background: Changes in protein glycosylation have been reported in various diseases, including cancer; however, the consequences of altered glycosylation in meningiomas remains undefined. We established two benign meningioma cell lines-SUT-MG12 and SUT-MG14, WHO grade I-and demonstrated the glycan and glycosyltransferase profiles of the mucin-type O-linked glycosylation in the primary benign meningioma cells compared with two malignant meningioma cell lines-HKBMM and IOMM-Lee, WHO grade III. Changes in O-linked glycosylation profiles in malignant meningiomas were proposed.\nMethods: Primary culture technique, morphological analysis, and immunocytochemistry were used to establish and characterize two benign meningioma cell lines. The glycan profiles of the primary benign and malignant meningiomas cell lines were then analyzed using lectin cytochemistry. The gene expression of O-linked glycosyltransferases, mucins, sialyltransferases, and fucosyltransferases were analyzed in benign and malignant meningioma using the GEO database (GEO series GSE16581) and quantitative-PCR (qPCR).\nResults: Lectin cytochemistry revealed that the terminal galactose (Gal) and N-acetyl galactosamine (GalNAc) were highly expressed in primary benign meningioma cells (WHO grade I) compared to malignant meningioma cell lines (WHO grade III). The expression profile of mucin types O-glycosyltransferases in meningiomas were observed through the GEO database and gene expression experiment in meningioma cell lines. In the GEO database, C1GALT1-specific chaperone (COSMC) and mucin 1 (MUC1) were significantly increased in malignant meningiomas (Grade II and III) compared with benign meningiomas (Grade I). Meanwhile, in the cell lines, Core 2 β1,6-N-acetylglucosaminyltransferase-2 (C2GNT2) was highly expressed in malignant meningiomas. We then investigated the complex mucin-type O-glycans structures by determination of sialyltransferases and fucosyltransferases. We found ST3 β-galactoside α-2,3-sialyltransferase 4 (ST3GAL4) was significantly decreased in the GEO database, while ST3GAL1, ST3GAL3, α1,3 fucosyltransferases 1 and 8 (FUT1 and FUT8) were highly expressed in malignant meningioma cell lines-(HKBMM)-compared to primary benign meningioma cells-(SUT-MG12 and SUT-MG14).\nConclusion: Our findings are the first to demonstrate the potential glycosylation changes in the O-linked glycans of malignant meningiomas compared with benign meningiomas, which may play an essential role in the progression, tumorigenesis, and malignancy of meningiomas.", "claims": [{"id": "claim-method", "statement": "The independent t-test was used to determine the differential expression of glycosyltransferase genes between the primary benign meningiomas and the malignant meningiomas.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38274327", "locator": "sections[8] \"Statistical analysis\" paragraph 2", "quote": "The independent t-test was used to determine the differential expression of glycosyltransferase genes between the primary benign meningiomas and the malignant meningiomas."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw data of Figures 5, 6, 7, and 8 are available in the Supplemental Files.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38274327", "locator": "sections[23] \"Data Availability\" paragraph 2", "quote": "The raw data of Figures 5, 6, 7, and 8 are available in the Supplemental Files."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper found increased expression of selected O-linked glycosylation-related genes in malignant meningiomas and proposed that these changes may contribute to meningioma progression and malignancy.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38274327", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Our findings are the first to demonstrate the potential glycosylation changes in the O-linked glycans of malignant meningiomas compared with benign meningiomas, which may play an essential role in the progression, tumorigenesis, and malignancy of meningiomas."}, {"asset": "source:pmid:38274327", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The gene expression of O-linked glycosyltransferases, mucins, sialyltransferases, and fucosyltransferases were analyzed in benign and malignant meningioma using the GEO database (GEO series GSE16581) and quantitative-PCR (qPCR)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The paper found increased expression of selected O-linked glycosylation-related genes in malignant meningiomas and proposed that these changes may contribute to meningioma progression and malignancy.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38274327"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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