Unveiling Differential Responses of Granulocytes to Distinct Immunostimulants with Implications in Autoimmune Uveitis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38275380", "kind": "science-record", "revision": 2, "content_hash": "sha256:67e5a7f49c45037d77d8de61d6bf4746d27eec09536a90eec01ad66aaa08aff0", "created_at": "2026-11-01T19:20:12Z", "updated_at": "2026-11-01T21:32:23Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Unveiling Differential Responses of Granulocytes to Distinct Immunostimulants with Implications in Autoimmune Uveitis.", "doi": "10.3390/biomedicines12010019", "pmid": "38275380", "authors": ["Roxane L Degroote", "Adrian Schmalen", "Stefanie M Hauck", "Cornelia A Deeg"], "journal": "Biomedicines", "year": 2023, "abstract": "The perception of circulating granulocytes as cells with a predetermined immune response mainly triggered by pathogens is evolving, recognizing their functional heterogeneity and adaptability, particularly within the neutrophil subset. The involvement of these cells in the pathophysiology of autoimmune uveitis has become increasingly clear, yet their exact role remains elusive. We used an equine model for autoimmune-mediated recurrent pan-uveitis to investigate early responses of granulocytes in different inflammatory environments. For this purpose, we performed differential proteomics on granulocytes from healthy and diseased horses stimulated with IL8, LPS, or PMA. Compared to healthy horses, granulocytes from the recurrent uveitis model significantly changed the cellular abundance of 384 proteins, with a considerable number of specific changes for each stimulant. To gain more insight into the functional impact of these stimulant-specific proteome changes in ERU pathogenesis, we used Ingenuity Pathway Analysis for pathway enrichment. This resulted in specific reaction patterns for each stimulant, with IL8 predominantly promoting Class I MHC-mediated antigen processing and presentation, LPS enhancing processes in phospholipid biosynthesis, and PMA, clearly inducing neutrophil degranulation. These findings shed light on the remarkably differentiated responses of neutrophils, offering valuable insights into their functional heterogeneity in a T-cell-driven disease. Raw data are available via ProteomeXchange with identifier PXD013648.", "claims": [{"id": "claim-method", "statement": "Significance of the ratios was tested using a background-based t-test with correction for multiple testing according to Benjamini-Hochberg (adjusted p-value) [34,35].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38275380", "locator": "sections[5] \"2.3. Data Processing and Label-Free Quantification\" paragraph 3", "quote": "Significance of the ratios was tested using a background-based t-test with correction for multiple testing according to Benjamini-Hochberg (adjusted p-value) [34,35]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org (accessed on on 15 January 2020)) via the PRIDE partner repository [94] with the dataset identifier PXD013648.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38275380", "locator": "sections[18] \"Data Availability Statement\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org (accessed on on 15 January 2020)) via the PRIDE partner repository [94] with the dataset identifier PXD013648."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Compared with healthy horses, granulocytes from horses with recurrent uveitis showed significant changes in the abundance of 384 proteins, with numerous stimulus-specific changes.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38275380", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Compared to healthy horses, granulocytes from the recurrent uveitis model significantly changed the cellular abundance of 384 proteins, with a considerable number of specific changes for each stimulant."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Compared with healthy horses, granulocytes from horses with recurrent uveitis showed significant changes in the abundance of 384 proteins, with numerous stimulus-specific changes.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38275380"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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