Histone Lactylation Participates in Psoriasis Progression by Regulating the Adiponectin Expression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38292324", "kind": "science-record", "revision": 4, "content_hash": "sha256:c543deb15e41813706a73f9c034a6159de9533196f01c3b2534b1e728625c6b5", "created_at": "2026-11-01T19:20:13Z", "updated_at": "2026-11-01T22:53:34Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Histone Lactylation Participates in Psoriasis Progression by Regulating the Adiponectin Expression.", "doi": "10.2147/CCID.S450254", "pmid": "38292324", "authors": ["Sicheng Zhao", "Tingyan Wu", "Mingjing Fu", "Zhe Zhang"], "journal": "Clinical, cosmetic and investigational dermatology", "year": 2024, "abstract": "Background: Psoriasis is a chronic inflammatory skin disease characterized by erythema, papules, and plaques. Adiponectin (ADIPOQ) is an important protein hormone secreted by adipose tissue. Here, we aimed to explore the expression of ADIPOQ in psoriasis patients and the moderation effect of histone lactylation on ADIPOQ.\nMethods: The GSE78097 data set was downloaded from GEO database to analyze the differentially expressed genes (DEGs) in psoriasis. A total of 36 psoriasis patients were recruited to obtain the skin samples. The ADIPOQ protein levels, global lactylation and histone lactylation (H3K18lac) levels were detected by Western blot assay. Chromatin immunoprecipitation (CHIP) assay was performed to detect the combination between H3K18lac and promoter regions of the ADIPOQ. The receiver operating curve (ROC) analysis was used to evaluate the diagnostic value of ADIPOQ in psoriasis.\nResults: ADIPOQ was decreased in the skin tissues of psoriasis patients. In addition, the global lactylation and H3K18lac levels were significantly decreased in the skin tissues of psoriasis patients. In HaCaT cells, promoting the global lactylation and H3K18lac levels increased the ADIPOQ protein levels, while si-LDHA transfection decreased the ADIPOQ protein levels. The CHIP results indicated that lactylation promoted the binding of promoter regions of the ADIPOQ and H3K18lac. Finally, the ROC analysis showed that ADIPOQ exhibited diagnostic value in psoriasis.\nConclusion: This study demonstrated ADIPOQ was decreased in the skin tissues of psoriasis patients, and ADIPOQ has diagnostic value for psoriasis. Furthermore, down-regulation of H3K18lac levels inhibited the transcription of ADIPOQ, which was the key factor of decrease of ADIPOQ levels in psoriasis patients.", "claims": [{"id": "claim-method", "statement": "Student’s t-test or one-way analysis of variance was used for two groups or multiple group comparisons, respectively. Additionally, the receiver operating curve (ROC) analysis was used to analyze the area under the curve (AUC) of ADIPOQ, in order to evaluate the diagnostic value of ADIPOQ in psoriasis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38292324", "locator": "sections[9] \"Statistical Analysis\" paragraph 2", "quote": "Student’s t-test or one-way analysis of variance was used for two groups or multiple group comparisons, respectively. Additionally, the receiver operating curve (ROC) analysis was used to analyze the area under the curve (AUC) of ADIPOQ, in order to evaluate the diagnostic value of ADIPOQ in psoriasis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38292324", "locator": "sections[17] \"Data Sharing Statement\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "ADIPOQ was decreased in the skin tissues of psoriasis patients, and ADIPOQ has diagnostic value for psoriasis.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38292324", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "This study demonstrated ADIPOQ was decreased in the skin tissues of psoriasis patients, and ADIPOQ has diagnostic value for psoriasis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "ADIPOQ was decreased in the skin tissues of psoriasis patients, and ADIPOQ has diagnostic value for psoriasis.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38292324"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE78097"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE78097"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:38292324", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/CCID.S450254", "sha256": "sha256:763114b975f7f13dbf021fc3d28d11f611fc91d4a9fb27db0854a6d3b7de6424", "bytes": 29305, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.2147/CCID.S450254", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10826714", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE78097", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78097", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE78097", "version": null, "note": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM2066635", "GSM2066636", "GSM2066637", "GSM2066638", "GSM2066639", "GSM2066640", "GSM2066641", "GSM2066642", "GSM2066643", "GSM2066644", "GSM2066645", "GSM2066646", "GSM2066647", "GSM2066648", "GSM2066649", "GSM2066650", "GSM2066651", "GSM2066652", "GSM2066653", "GSM2066654", "GSM2066655", "GSM2066656", "GSM2066657", "GSM2066658", "GSM2066659", "GSM2066660", "GSM2066661", "GSM2066662", "GSM2066663", "GSM2066664", "GSM2066665", "GSM2066666", "GSM2066667"], "sample_count": 33, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "The spectrum of mild-to-severe psoriasis vulgaris is defined by a common activation of IL-17 pathway genes, but with key differences in immune regulatory genes", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE78097 not downloaded", "asset:data:geo:GSE78097 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Student’s t-test or one-way analysis of variance was used for two groups or multiple group comparisons, respectively. Additionally, the receiver operating curve (ROC) analysis was used to analyze the area under the curve (AUC) of ADIPOQ, in order to evaluate the diagnostic value of ADIPOQ in psoriasis."}}, {"id": "method-2", "family": "network_analysis", "specific": "ppi_network", "label": "Network / pathway analysis", "dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.8, "band": "high", "quote": "PPI"}}, {"id": "method-3", "family": "diagnostic_evaluation", "specific": "roc", "label": "Diagnostic test evaluation", "dsl_operations": ["confusion_counts", "roc_curve", "sensitivity_specificity", "solve_confusion_matrix", "classification_metrics", "published_precision_envelope"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["reference_standard_and_test"], "evidence": {"score": 0.7, "band": "medium", "quote": "ROC analysis"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: anova."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE78097"], "resolved_accessions": ["GSE78097"], "statements": ["The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request."], "basis": "The paper names GSE78097 in GEO database and the accession resolves in the repository. 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Chromatin immunoprecipitation (CHIP)", "riasis patients were detected by RT-qPCR and Western blot assays. Lactylation Level Was Up-Regulated i", "he Psoriasis Patients Next, we performed the Western blot using Anti-L-Lactyl. The results showed that", "levels in the skin samples were detecting by Western blot assay using Anti-L-Lactyl. H3K18lac level wa"], "order_basis": "paper_text_order:691", "id": "assay:1"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["sed the ADIPOQ protein levels, while si-LDHA transfection decreased the ADIPOQ protein levels. The CHI", "d the cells were cultured for 24 h. For cell transfection, the lactate dehydrogenase A (LDHA) knockout", "(Shanghai, China). The si-LDHA and si-nc was transfected into cells using Lipofectamine 3000 (Invitro", "the lactate dehydrogenase A (LDHA) knockout siRNA (si-LDHA) and negative control (si-nc) were"], "order_basis": "paper_text_order:1278", "id": "assay:2"}, {"role": "data_generation", "definition_id": "elisa", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["collected for ADIPOQ levels determination by ELISA kit (Human Leptin Quantikine Elisa Kit, COIN", "nation by ELISA kit (Human Leptin Quantikine Elisa Kit, COINO BIO, Shanghai, China) according t", "lasma of psoriasis patients were detected by ELISA kit. The mRNA (F) and protein (G) levels of"], "order_basis": "paper_text_order:2539", "id": "assay:3"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["f Sciences (Shanghai, China). The cells were cultured in a complete medium (DMEM; Invitrogen, USA) su"], "order_basis": "paper_text_order:3243", "id": "assay:4"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["mples of psoriasis patients were detected by RT-qPCR and Western blot assays. Lactylation Level W", "es of psoriasis patients were detected by RT-qPCR and Western blot assays. Lactylation Level W"], "order_basis": "paper_text_order:5922", "id": "assay:5"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Student’s t-test or one-way analysis of variance was used for two groups or multiple group comparisons, respectively. Additionally, the receiver operating curve (ROC) analysis was used to analyze the area under the curve (AUC) of ADIPOQ, in order to evaluate the diagnostic value of ADIPOQ in psoriasis."], "order_basis": "consumes_earlier_output", "id": "assay:6"}]}