New Function Annotation of PROSER2 in Pancreatic Ductal Adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38293943", "kind": "science-record", "revision": 2, "content_hash": "sha256:8538af66baf81426de465b0ce32f736fade8f07a2613c458a2dc8bac3cbc77ee", "created_at": "2026-11-01T19:20:13Z", "updated_at": "2026-11-01T21:32:25Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "New Function Annotation of PROSER2 in Pancreatic Ductal Adenocarcinoma.", "doi": "10.1021/acs.jproteome.3c00632", "pmid": "38293943", "authors": ["Yu-Sun Lee", "Jieun Im", "Yeji Yang", "Hea Ji Lee", "Mi Rim Lee", "Sang-Myung Woo", "Sang-Jae Park", "Sun-Young Kong", "Jin Young Kim", "Heeyoun Hwang", "Yun-Hee Kim"], "journal": "Journal of proteome research", "year": 2024, "abstract": "Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis due to the absence of diagnostic markers and molecular targets. Here, we took an unconventional approach to identify new molecular targets for pancreatic cancer. We chose uncharacterized protein evidence level 1 without function annotation from extensive proteomic research on pancreatic cancer and focused on proline and serine-rich 2 (PROSER2), which ranked high in the cell membrane and cytoplasm. In our study using cell lines and patient-derived orthotopic xenograft cells, PROSER2 exhibited a higher expression in cells derived from primary tumors than in those from metastatic tissues. PROSER2 was localized in the cell membrane and cytosol by immunocytochemistry. PROSER2 overexpression significantly reduced the metastatic ability of cancer cells, whereas its suppression had the opposite effect. Proteomic analysis revealed that PROSER2 interacts with STK25 and PDCD10, and their binding was confirmed by immunoprecipitation and immunocytochemistry. STK25 knockdown enhanced metastasis by decreasing p-AMPK levels, whereas PROSER2-overexpressing cells increased the level of p-AMPK, indicating that PROSER2 suppresses invasion via the AMPK pathway by interacting with STK25. This is the first demonstration of the novel role of PROSER2 in antagonizing tumor progression via the STK25-AMPK pathway in PDAC. LC-MS/MS data are available at MassIVE (MSV000092953) and ProteomeXchange (PXD045646).", "claims": [{"id": "claim-method", "statement": "The p-value was calculated using the student’s t test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38293943", "locator": "sections[12] \"Statistics\" paragraph 2", "quote": "The p-value was calculated using the student’s t test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Proteome data is available at MassIVE (MSV000092953) and ProteomeXchange (PXD045646).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38293943", "locator": "sections[22] \"Data Availability Statement\" paragraph 2", "quote": "Proteome data is available at MassIVE (MSV000092953) and ProteomeXchange (PXD045646)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "PROSER2 suppresses invasion via the AMPK pathway by interacting with STK25.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38293943", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "STK25 knockdown enhanced metastasis by decreasing p-AMPK levels, whereas PROSER2-overexpressing cells increased the level of p-AMPK, indicating that PROSER2 suppresses invasion via the AMPK pathway by interacting with STK25."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "PROSER2 suppresses invasion via the AMPK pathway by interacting with STK25.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38293943"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_unresolved", "description": "The GEO series accession resolves and its sample accessions are listed. 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LC-MS/MS data are available at MassIVE (MSV000092953)", "bs, Berlin, Germany). Sample Preparation for Mass Spectrometry Analysis Cells were lysed with 1× sodium dod", "ormic acid) for liquid chromatography-tandem mass spectrometry (LC–MS/MS) analysis. Data Processing and Bio", "d by combined analyses using Orbitrap Fusion mass spectrometry (Figure 3A). A total of 4889 proteins were i"], "order_basis": "paper_text_order:345", "id": "assay:1"}, {"role": "data_generation", "definition_id": "clinical_cohort", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["cytoplasm. In our study using cell lines and patient-derived orthotopic xenograft cells, PROSER2 exhibite", "n. PDOXc were isolated from tumor tissues of patient-derived orthotopic xenograft (PDOX) models: SPDOXc f"], "order_basis": "paper_text_order:525", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["precipitation and immunocytochemistry. STK25 knockdown enhanced metastasis by decreasing p-AMPK lev", "ween PROSER2-overexpressing and blank vector-transfected cells. 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