Bioinformatic analysis reveals prognostic value and immunotherapy potential of Siglec-15 in laryngeal squamous cell carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38352733", "kind": "science-record", "revision": 4, "content_hash": "sha256:f5d650cfbc621e2c56907bb155bff0b7711c46e1679fba6bffe477c81756d210", "created_at": "2026-11-01T19:20:17Z", "updated_at": "2026-11-01T22:53:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Bioinformatic analysis reveals prognostic value and immunotherapy potential of Siglec-15 in laryngeal squamous cell carcinoma.", "doi": "10.1016/j.heliyon.2024.e25266", "pmid": "38352733", "authors": ["Xiaoting Chen", "Qian Cai", "Kaiyi Wong", "Ximing Shen", "Zhong Guan"], "journal": "Heliyon", "year": 2024, "abstract": "Background: Laryngeal squamous cell carcinoma (LSCC) is the ultimate common malignant head and neck cancer with dismal prognosis. The expression pattern and clinical significance of Siglec-15 (Sialic acid-binding immunoglobulin-like lectin 15) in LSCC are poorly understood. In order to lay the groundwork for future immune-related research on Siglec-15 in LSCC, we set out to study its expression and prognostic importance in the disease, as well as to use bioinformatics to investigate the immune features modulated by Siglec-15 in LSCC.\nMethods: ① In order to get the gene expression profile and clinical data for TCGA head and neck cancer (TCGA-HNSC), you may access the relevant data from UCSC xena and use 110 cases of laryngeal cancer as a training set. Two datasets, GSE27020 and GSE25727, were obtained from the GEO databank and utilized as validation sets. These datasets include expression profiles and clinical information. The Siglec-15 gene and immune characteristics were analyzed by bioinformatics methods. ② Retrospectively collected routine paraffin specimens from patients with pathological diagnosis of squamous cell carcinoma from December 2012 to November 2015 in Sun Yat-sen Memorial Hospital and fresh frozen tissue of patients from June 2021 to March 2022. Immunohistochemistry method, immunofluorescence technique and real-time quantitative PCR was used to examine the difference of Siglec-15 appearance in LSCC tissue and adjacent tissue, and its correlation of prognosis, clinic pathological characteristics and CD8+T lymphocyte infiltration. Using human laryngeal cancer cell line (LCC), we studied the influence of Siglec-15 in cell proliferation and invasion.\nResults: We identified Siglec-15 was upregulated in LSCC. The patients in Siglec-15 high expression group had a poor overall survival (OS) based on the clinical information from TGCA and 111 LSCC patients that hospitalized in Sun Yat-sen Memorial Hospital. The COX regression analysis indicated Siglec-15 as an independent predictor for poor prognosis of LSCC. Bioinformatic analysis suggested that the high expression of Siglec-15 shape an immune suppressive tumor microenvironment (TEM), leading to poor response to immunotherapy in LSCC. Siglec-15 enhanced cell invasion and proliferation, as we showed in vitro.\nConclusion: Our study support Siglec-15 as a potential predictor for LSCC prognosis and an attractive target for LSCC immunotherapy.", "claims": [{"id": "claim-method", "statement": "Kaplan-Meier estimator was utilized to build the persistence function of each group. Log-rank test was applied to relate the survival curves created by the Kaplan-Meier estimator to assess the influence of Siglec-15 on overall survival (OS).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38352733", "locator": "sections[4] \"Kaplan-Meier survival analysis\" paragraph 2", "quote": "Kaplan-Meier estimator was utilized to build the persistence function of each group. Log-rank test was applied to relate the survival curves created by the Kaplan-Meier estimator to assess the influence of Siglec-15 on overall survival (OS)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data will be made available on request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38352733", "locator": "sections[29] \"Data availability statement\" paragraph 2", "quote": "Data will be made available on request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "According to Kaplan-Meier investigation, patients whose Siglec-15 expression was high had a substantially lower overall survival (OS) compared to those whose expression was low (29.7 ± 28.9 months vs. 34.9 ± 32.2 months, P = 0.030, Fig. 1B).", "kind": "quantitative", "target": {"value": 0.03, "unit": "p-value", "tolerance": null, "source": "According to Kaplan-Meier investigation, patients whose Siglec-15 expression was high had a substantially lower overall survival (OS) compared to those whose expression was low (29.7 ± 28.9 months vs. 34.9 ± 32.2 months, P = 0.030, Fig. 1B)."}, "evidence": [{"asset": "source:pmid:38352733", "locator": "sections[18] \"Association of Siglec-15 with overall survival in LSCC\" paragraph 2", "quote": "According to Kaplan-Meier investigation, patients whose Siglec-15 expression was high had a substantially lower overall survival (OS) compared to those whose expression was low (29.7 ± 28.9 months vs. 34.9 ± 32.2 months, P = 0.030, Fig. 1B)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "According to Kaplan-Meier investigation, patients whose Siglec-15 expression was high had a substantially lower overall survival (OS) compared to those whose expression was low (29.7 ± 28.9 months vs. 34.9 ± 32.2 months, P = 0.030, Fig. 1B).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38352733"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Immunohistochemistry method, immunofluorescence technique and rea", "to March 2022. Immunohistochemistry method, immunofluorescence technique and real-time quantitative PCR was", "cal features of patients with LSCC. Based on immunohistochemistry data, 111 cases of LSCC were split into two", "Quantification of fluorescence intensity and immunofluorescence images exhibiting a significant negative cor"], "order_basis": "paper_text_order:1364", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["try method, immunofluorescence technique and real-time quantitative PCR was used to examine the difference of Siglec"], "order_basis": "paper_text_order:1426", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Methods and materials Cell culture and transfection The human LCC (laryngeal cancer cell) line w", "chnology company (Shanghai, China). LCC were transfected with shSiglec-15 and pLVX-Siglec-15 using Li"], "order_basis": "paper_text_order:2567", "id": "assay:3"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["survival_km"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Kaplan-Meier estimator was utilized to build the persistence function of each group. Log-rank test was applied to relate the survival curves created by the Kaplan-Meier estimator to assess the influence of Siglec-15 on overall survival (OS)."], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}