Discovery of plasma proteome markers associated with clinical outcome and immunological stress after cardiac surgery.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38379859", "kind": "science-record", "revision": 3, "content_hash": "sha256:970423263a398dab39896a4d8fd4fc7ca809d0cc329346d7db4b0a8daeda0093", "created_at": "2026-11-01T19:20:19Z", "updated_at": "2026-11-01T21:32:33Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Discovery of plasma proteome markers associated with clinical outcome and immunological stress after cardiac surgery.", "doi": "10.3389/fcvm.2023.1287724", "pmid": "38379859", "authors": ["Corina Bello", "Mark G Filipovic", "Markus Huber", "Sarah Flannery", "Beatrice Kobel", "Roman Fischer", "Benedikt M Kessler", "Lorenz Räber", "Frank Stueber", "Markus M Luedi"], "journal": "Frontiers in cardiovascular medicine", "year": 2023, "abstract": "Background: Molecular mechanisms underlying perioperative acute phase reactions in cardiac surgery are largely unknown. We aimed to characterise perioperative alterations of the acute phase plasma proteome in a cohort of adult patients undergoing on-pump cardiac surgery using high-throughput mass spectrometry and to identify candidate proteins potentially relevant to postoperative clinical outcome through a novel, multi-step approach.\nMethods: This study is an analysis of the Bern Perioperative Biobank, a prospective cohort of adults who underwent cardiac surgery with the use of cardiopulmonary bypass (CPB) at Bern University Hospital between January and December 2019. Blood samples were taken before induction of anaesthesia and on postoperative day one. Proteomic analyses were performed by mass spectrometry. Through a multi-step, exploratory approach, hit-proteins were first identified according to their perioperative prevalence and dynamics. The set of hit-proteins were associated with predefined clinical outcome measures (all-cause one-year mortality, length of hospital stay, postoperative myocardial infarction and stroke until hospital discharge).\nResults: 192 patients [75.5% male, median age 67.0 (IQR 60.0-73.0)] undergoing cardiac surgery with the use of CPB were included in this analysis. In total, we identified and quantified 402 proteins across all samples, whereof 30/402 (7%) proteins were identified as hit-proteins. Three hit-proteins-LDHB, VCAM1 and IGFBP2-demonstrated the strongest associations with clinical outcomes. After adjustment both for age, sex, BMI and for multiple comparisons, the scaled preoperative levels of IGFBP2 were associated with 1-year all-cause mortality (OR 10.63; 95% CI: 2.93-64.00; p = 0.046). Additionally, scaled preoperative levels of LDHB (OR 5.58; 95% CI: 2.58-8.57; p = 0.009) and VCAM1 (OR 2.32; 95% CI: 0.88-3.77; p = 0.05) were found to be associated with length of hospital stay.\nConclusions: We identified a subset of promising candidate plasma proteins relevant to outcome after on-pump cardiac surgery. IGFBP2 showed a strong association with clinical outcome measures and a significant association of preoperative levels with 1-year all-cause mortality. Other proteins strongly associated with outcome were LDHB and VCAM1, reflecting the dynamics in the acute phase response, inflammation and myocardial injury. We recommend further investigation of these proteins as potential outcome markers after cardiac surgery.\nClinical Trial Registration: ClinicalTrials.gov; NCT04767685, data are available via ProteomeXchange with identifier PXD046496.", "claims": [{"id": "claim-method", "statement": "For the final set of hit proteins, we computed both crude and adjusted (age, gender and BMI) odds ratios (ORs) with the four clinical outcomes of interest by means of univariable and multivariable logistic regression. For each clinical outcome individually, the p-values resulting from this analysis were adjusted for multiple comparison by means of the Bonferroni correction.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38379859", "locator": "sections[7] \"Outcome analysis\" paragraph 2", "quote": "For the final set of hit proteins, we computed both crude and adjusted (age, gender and BMI) odds ratios (ORs) with the four clinical outcomes of interest by means of univariable and multivariable logistic regression. For each clinical outcome individually, the p-values resulting from this analysis were adjusted for multiple comparison by means of the Bonferroni correction."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ebi.ac.uk/pride/archive/projects/PXD046496.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38379859", "locator": "sections[13] \"Data availability statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ebi.ac.uk/pride/archive/projects/PXD046496."}], "state": "complete"}, {"id": "claim-target-result", "statement": "After adjustment both for age, sex, BMI and for multiple comparisons, the scaled preoperative levels of IGFBP2 were associated with 1-year all-cause mortality (OR 10.63; 95% CI: 2.93–64.00; p = 0.046).", "kind": "quantitative", "target": {"value": 95.0, "unit": "per cent", "tolerance": null, "source": "After adjustment both for age, sex, BMI and for multiple comparisons, the scaled preoperative levels of IGFBP2 were associated with 1-year all-cause mortality (OR 10.63; 95% CI: 2.93–64.00; p = 0.046)."}, "evidence": [{"asset": "source:pmid:38379859", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "After adjustment both for age, sex, BMI and for multiple comparisons, the scaled preoperative levels of IGFBP2 were associated with 1-year all-cause mortality (OR 10.63; 95% CI: 2.93–64.00; p = 0.046)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "After adjustment both for age, sex, BMI and for multiple comparisons, the scaled preoperative levels of IGFBP2 were associated with 1-year all-cause mortality (OR 10.63; 95% CI: 2.93–64.00; p = 0.046).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38379859"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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