Regional Differences in the Small Intestinal Proteome of Control Mice and of Mice Lacking Lysosomal Acid Lipase.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38422518", "kind": "science-record", "revision": 5, "content_hash": "sha256:38fb57fb3193c522295aa2cb8a9107c9d18f0e3b1136468a4ae4814f0fcbd025", "created_at": "2026-11-01T19:20:22Z", "updated_at": "2026-11-01T22:53:47Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Regional Differences in the Small Intestinal Proteome of Control Mice and of Mice Lacking Lysosomal Acid Lipase.", "doi": "10.1021/acs.jproteome.4c00082", "pmid": "38422518", "authors": ["Valentina Bianco", "Monika Svecla", "Giovanni Battista Vingiani", "Dagmar Kolb", "Birgit Schwarz", "Martin Buerger", "Giangiacomo Beretta", "Giuseppe Danilo Norata", "Dagmar Kratky"], "journal": "Journal of proteome research", "year": 2024, "abstract": "The metabolic contribution of the small intestine (SI) is still unclear despite recent studies investigating the involvement of single cells in regional differences. Using untargeted proteomics, we identified regional characteristics of the three intestinal tracts of C57BL/6J mice and found that proteins abundant in the mouse ileum correlated with the high ileal expression of the corresponding genes in humans. In the SI of C57BL/6J mice, we also detected an increasing abundance of lysosomal acid lipase (LAL), which is responsible for degrading triacylglycerols and cholesteryl esters within the lysosome. LAL deficiency in patients and mice leads to lipid accumulation, gastrointestinal disturbances, and malabsorption. We previously demonstrated that macrophages massively infiltrated the SI of Lal-deficient (KO) mice, especially in the duodenum. Using untargeted proteomics (ProteomeXchange repository, data identifier PXD048378), we revealed a general inflammatory response and a common lipid-associated macrophage phenotype in all three intestinal segments of Lal KO mice, accompanied by a higher expression of GPNMB and concentrations of circulating sTREM2. However, only duodenal macrophages activated a metabolic switch from lipids to other pathways, which were downregulated in the jejunum and ileum of Lal KO mice. Our results provide new insights into the process of absorption in control mice and possible novel markers of LAL-D and/or systemic inflammation in LAL-D.", "claims": [{"id": "claim-method", "statement": "Comparison between two groups was performed using unpaired Student’s t-test, and comparisons of multiple groups were analyzed by two-way ANOVA, followed by Tukey’s post hoc test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38422518", "locator": "sections[12] \"Statistics\" paragraph 2", "quote": "Comparison between two groups was performed using unpaired Student’s t-test, and comparisons of multiple groups were analyzed by two-way ANOVA, followed by Tukey’s post hoc test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data sets are available at the ProteomeXchange Consortium via the PRIDE partner repository51 under the data set identifier PXD048378. 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