Analysis of Key Genes and miRNA-mRNA Networks Associated with Glucocorticoids Treatment in Chronic Obstructive Pulmonary Disease.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38435123", "kind": "science-record", "revision": 4, "content_hash": "sha256:efc3a783c62851afa82bdfe686ff94d89f122473f270d1aaecfab4d76ec20aa9", "created_at": "2026-11-01T19:20:22Z", "updated_at": "2026-11-01T22:53:48Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Analysis of Key Genes and miRNA-mRNA Networks Associated with Glucocorticoids Treatment in Chronic Obstructive Pulmonary Disease.", "doi": "10.2147/COPD.S441716", "pmid": "38435123", "authors": ["Jian-Jun Wu", "Ping-An Zhang", "Ming-Zhe Chen", "Yi Zhang", "Wei-Sha Du", "Xiao-Ning Li", "Guo-Chao Ji", "Liang-Duo Jiang", "Yang Jiao", "Xin Li"], "journal": "International journal of chronic obstructive pulmonary disease", "year": 2024, "abstract": "Background: Some patients with chronic obstructive pulmonary disease (COPD) benefit from glucocorticoid (GC) treatment, but its mechanism is unclear.\nObjective: With the help of the Gene Expression Omnibus (GEO) database, the key genes and miRNA-mRNA related to the treatment of COPD by GCs were discussed, and the potential mechanism was explained.\nMethods: The miRNA microarray dataset (GSE76774) and mRNA microarray dataset (GSE36221) were downloaded, and differential expression analysis were performed. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on the differentially expressed genes (DEGs). The protein interaction network of the DEGs in the regulatory network was constructed with the STRING database, and the key genes were screened through Cytoscape. Potential downstream target genes regulated by differentially expressed miRNAs (DEMs) were predicted by the miRWalk3.0 database, and miRNA-mRNA regulatory networks were constructed. Finally, some research results were validated.\nResults: ① Four DEMs and 83 DEGs were screened; ② GO and KEGG enrichment analysis mainly focused on the PI3K/Akt signalling pathway, ECM receptor interaction, etc.; ③ CD2, SLAMF7, etc. may be the key targets of GC in the treatment of COPD; ④ 18 intersection genes were predicted by the mirwalk 3.0 database, and 9 pairs of miRNA-mRNA regulatory networks were identified; ⑤ The expression of miR-320d-2 and TFCP2L1 were upregulated by dexamethasone in the COPD cell model, while the expression of miR-181a-2-3p and SLAMF7 were downregulated.\nConclusion: In COPD, GC may mediate the expression of the PI3K/Akt signalling pathway through miR-181a-2-3p, miR-320d-2, miR-650, and miR-155-5p, targeting its downstream signal factors. The research results provide new ideas for RNA therapy strategies of COPD, and also lay a foundation for further research.", "claims": [{"id": "claim-method", "statement": "The microarray data of miRNA and mRNA expression profiles before treatment and 6 months and 30 months after treatment were analysed by the limma package of R software.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38435123", "locator": "sections[5] \"Data Processing and Differential Expression Analysis\" paragraph 2", "quote": "The microarray data of miRNA and mRNA expression profiles before treatment and 6 months and 30 months after treatment were analysed by the limma package of R software."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data supporting the findings of this study are available within the article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38435123", "locator": "sections[30] \"Data Sharing Statement\" paragraph 2", "quote": "The data supporting the findings of this study are available within the article."}], "state": "complete"}, {"id": "claim-target-result", "statement": "① Four DEMs and 83 DEGs were screened;", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38435123", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "① Four DEMs and 83 DEGs were screened;"}, {"asset": "source:pmid:38435123", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "The miRNA microarray dataset (GSE76774) and mRNA microarray dataset (GSE36221) were downloaded, and differential expression analysis were performed."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "① Four DEMs and 83 DEGs were screened;", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38435123"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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