In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38468318", "kind": "science-record", "revision": 3, "content_hash": "sha256:f2f52f51a4d77f3940d155266900bfced8258154cf812c7695544ff2ea48d29c", "created_at": "2026-11-01T19:20:24Z", "updated_at": "2026-11-01T21:32:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig.", "doi": "10.1186/s40104-024-01002-x", "pmid": "38468318", "authors": ["Maria A Gil", "Josep M Cambra", "Heriberto Rodriguez-Martinez", "Cristina Cuello", "Inmaculada Parrilla", "Emilio A Martinez"], "journal": "Journal of animal science and biotechnology", "year": 2024, "abstract": "BACKGROUND: Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo cross-communication. In this study, the proteome of the endometrium and chorioallantoic membrane was characterized in pregnant sows (PS) during early gestation (d 18 and 24 of gestation) and in the endometrium of non-pregnant sows (NPS) during the same days using LC-MS/MS analysis. The UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks, respectively.\nRESULTS: Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS, respectively; of these, 1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS, respectively. In addition, we identified 3,968 proteins in the extraembryonic membranes of PS. Further analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization, suggesting they dominated the moment of endometrial remodeling, implantation and adhesion of the lining epithelia. Data are available via ProteomeXchange with identifier PXD042565.\nCONCLUSION: This is the first in-depth proteomic characterization of the endometrium and extraembryonic membranes during weeks 3 to 4 of gestation; data that contribute to the molecular understanding of the dynamic environment during this critical period, associated with the majority of pregnancy losses.", "claims": [{"id": "claim-method", "statement": "The following selection criteria were applied: (1) a P-value cut-off of at least 0.05, (2) use of the enrichment/depletion statistical test (two-sided hypergeometric test) with Bonferroni step down, minimum GO level 2, maximum GO level 6, and a kappa score threshold of 0.4, and (3) analysis of Homo sapiens organism with genes present in KEGG pathways, REACTOME pathways and GO Biological Process.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38468318", "locator": "sections[12] \"Data analysis\" paragraph 4", "quote": "The following selection criteria were applied: (1) a P-value cut-off of at least 0.05, (2) use of the enrichment/depletion statistical test (two-sided hypergeometric test) with Bonferroni step down, minimum GO level 2, maximum GO level 6, and a kappa score threshold of 0.4, and (3) analysis of Homo sapiens organism with genes present in KEGG pathways, REACTOME pathways and GO Biological Process."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data generated or analyzed during this study are included in this published article [and its supplementary information files]. Further inquiries can be directed to the corresponding author.\nThe mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [17] partner repository with the dataset identifier PXD042565.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38468318", "locator": "sections[29] \"Availability of data and materials\" paragraphs 1-3", "quote": "All data generated or analyzed during this study are included in this published article [and its supplementary information files]. Further inquiries can be directed to the corresponding author.\nThe mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [17] partner repository with the dataset identifier PXD042565."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified 3,254 endometrial proteins in pregnant sows, 3,457 in non-pregnant sows, and 3,968 in the extraembryonic membranes of pregnant sows.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38468318", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS, respectively; of these, 1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS, respectively. In addition, we identified 3,968 proteins in the extraembryonic membranes of PS."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified 3,254 endometrial proteins in pregnant sows, 3,457 in non-pregnant sows, and 3,968 in the extraembryonic membranes of pregnant sows.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38468318"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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The UniProtKB database and ClueGO", "be directed to the corresponding author. The mass spectrometry proteomics data have been deposited to the P", "2565. CONCLUSION: This is the first in-depth proteomic characterization of the endometrium and extr", "nts. Experimental design To characterize the proteomic profile of porcine endometrium and extra-emb"], "order_basis": "paper_text_order:461", "id": "assay:1"}]}