Exploring the role of mitochondrial-associated and peripheral neuropathy genes in the pathogenesis of diabetic peripheral neuropathy.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38481183", "kind": "science-record", "revision": 5, "content_hash": "sha256:415ff1f4b1906d96224cd0654856af80cbdda6248b1515d5eb7060b6b26eb46e", "created_at": "2026-11-01T19:20:25Z", "updated_at": "2026-11-01T22:53:53Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Exploring the role of mitochondrial-associated and peripheral neuropathy genes in the pathogenesis of diabetic peripheral neuropathy.", "doi": "10.1186/s12883-024-03589-0", "pmid": "38481183", "authors": ["Ruojing Bai", "Yuanyuan Luo"], "journal": "BMC neurology", "year": 2024, "abstract": "BACKGROUND: Diabetic peripheral neuropathy (DPN) is a prevalent and serious complication of diabetes mellitus, impacting the nerves in the limbs and leading to symptoms like pain, numbness, and diminished function. While the exact molecular and immune mechanisms underlying DPN remain incompletely understood, recent findings indicate that mitochondrial dysfunction may play a role in the advancement of this diabetic condition.\nMETHODS: Two RNA transcriptome datasets (codes: GSE185011 and GSE95849), comprising samples from diabetic peripheral neuropathy (DPN) patients and healthy controls (HC), were retrieved from the Gene Expression Omnibus (GEO) database hosted by the National Center for Biotechnology Information (NCBI). Subsequently, differential expression analysis and gene set enrichment analysis were performed. Protein-protein interaction (PPI) networks were constructed to pinpoint key hub genes associated with DPN, with a specific emphasis on genes related to mitochondria and peripheral neuropathy disease (PND) that displayed differential expression. Additionally, the study estimated the levels of immune cell infiltration in both the HC and DPN samples. To validate the findings, quantitative polymerase chain reaction (qPCR) was employed to confirm the differential expression of selected genes in the DPN samples.\nRESULTS: This research identifies four hub genes associated mitochondria or PN. Furthermore, the analysis revealed increased immune cell infiltration in DPN tissues, particularly notable for macrophages and T cells. Additionally, our investigation identified potential drug candidates capable of regulating the expression of the four hub genes. These findings were corroborated by qPCR results, reinforcing the credibility of our bioinformatics analysis.\nCONCLUSIONS: This study provides a comprehensive overview of the molecular and immunological characteristics of DPN, based on both bioinformatics and experimental methods.", "claims": [{"id": "claim-method", "statement": "The differentially expressed genes (DEGs) between normal tissues and disease tissues were identified using the moderated independent sample t-test from the “limma” package [8].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38481183", "locator": "sections[6] \"Differential expression analysis, functional enrichment analysis of GO and KEGG gene sets\" paragraph 2", "quote": "The differentially expressed genes (DEGs) between normal tissues and disease tissues were identified using the moderated independent sample t-test from the “limma” package [8]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "No datasets were generated or analysed during the current study.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38481183", "locator": "sections[33] \"Availability of data and materials\" paragraph 2", "quote": "No datasets were generated or analysed during the current study."}], "state": "complete"}, {"id": "claim-target-result", "statement": "This research identifies four hub genes associated mitochondria or PN.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38481183", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "This research identifies four hub genes associated mitochondria or PN."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "This research identifies four hub genes associated mitochondria or PN.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38481183"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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