Role of CCR1/5/7 in hepatocellular carcinoma: a study on prognostic evaluation, molecular subtyping, and association with immune infiltration.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38552222", "kind": "science-record", "revision": 4, "content_hash": "sha256:f5b4338be2fe3a08d8fb436691a9d59408290b87366fff2815e5e220788bd6ea", "created_at": "2026-11-01T19:20:31Z", "updated_at": "2026-11-01T22:54:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Role of CCR1/5/7 in hepatocellular carcinoma: a study on prognostic evaluation, molecular subtyping, and association with immune infiltration.", "doi": "10.18632/aging.205698", "pmid": "38552222", "authors": ["Huasheng Huang", "Chenlu Lan", "Yongguang Wei", "Jusen Nong", "Xiwen Liao", "Xinping Ye", "Ganlu Deng", "Tao Peng", "Xin Zhou"], "journal": "Aging", "year": 2024, "abstract": "This study aims to assess the prognostic value of the C-C motif chemokine receptor (CCR) gene family in hepatocellular carcinoma (HCC) and its relationship with immune infiltration and molecular subtypes of HCC. The evaluation of the GSE14520 dataset and TCGA database confirmed the prognostic significance of CCR. Building upon the correlation between CCR1, CCR5, and CCR7 and favorable prognosis, we further validated the prognostic importance of CCR1, CCR5, and CCR7 in ICGC database and an independent cohort from Guangxi autonomous region. Then, we constructed a risk prognosis model. Additionally, we observed significant positive correlations between CCR1, CCR5, and CCR7 and the infiltration of B cells, T cells, and macrophages in HCC. Subsequently, we conducted CCK assays, Transwell assays, and colony formation assays to evaluate the molecular biological functions of CCR1, CCR5, and CCR7. These experiments further confirmed that upregulation of CCR1, CCR5, and CCR7 can individually inhibit the proliferation, migration, and stemness of HCC cells. By analyzing the relationship between expression levels and tumor mutation frequency, we discovered that patients with high CCR1 expression were more likely to be classified as non-proliferative HCC. Similar conclusions were observed for CCR5 and CCR7. The association of CCR1, CCR5, and CCR7 with the molecular subtypes of HCC suggests that they may serve as intermediary molecules linking immune status and molecular subtypes in HCC. In summary, CCR1, CCR5, and CCR7 have the potential to serve as prognostic biomarkers for HCC and regulate HCC progression by influencing immune cell infiltration.", "claims": [{"id": "claim-method", "statement": "The Kaplan-Meier method along with the log-rank test and Cox proportional hazards model was respectively applied for the survival analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38552222", "locator": "sections[18] \"Statistical analysis\" paragraph 2", "quote": "The Kaplan-Meier method along with the log-rank test and Cox proportional hazards model was respectively applied for the survival analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38552222", "locator": "sections[19] \"Availability of data and material\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The difference between the high and low risk groups in OS was statistically significant (P=0.025, Figure 4L).", "kind": "quantitative", "target": {"value": 0.025, "unit": "p-value", "tolerance": null, "source": "The difference between the high and low risk groups in OS was statistically significant (P=0.025, Figure 4L)."}, "evidence": [{"asset": "source:pmid:38552222", "locator": "sections[25] \"Nomogram and prognostic signature\" paragraph 6", "quote": "The difference between the high and low risk groups in OS was statistically significant (P=0.025, Figure 4L)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The difference between the high and low risk groups in OS was statistically significant (P=0.025, Figure 4L).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38552222"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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