Identification of the Most Immunoreactive Antigens of Candida auris to IgGs from Systemic Infections in Mice.

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In this study, immunocompetent murine systemic infections caused by non-aggregative and aggregative phenotypes of C. auris and by Candida albicans and Candida haemulonii were carried out, and the obtained sera were used to study their immunoreactivity against C. auris proteins. The results showed higher virulence, in terms of infection signs, weight loss, and histopathological damage, of the non-aggregative isolate. Moreover, C. auris was less virulent than C. albicans but more than C. haemulonii. Regarding the immunoproteomics study, 13 spots recognized by sera from mice infected with both C. auris phenotypes and analyzed by mass spectrometry corresponded to enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate mutase. These four proteins were also recognized by sera obtained from human patients with disseminated C. auris infection but not by sera obtained from mice infected with C. albicans or Aspergillus fumigatus. Spot identification data are available via ProteomeXchange with the identifier PXD049077. In conclusion, this study showed that the identified proteins could be potential candidates to be studied as new diagnostic or even therapeutic targets for C. auris.", "claims": [{"id": "claim-method", "statement": "Statistical analyses were carried out using the ANOVA method followed by multiple comparisons corrected with Dunnett’s test in SPSS (version 26.0 for Windows, Chicago, Illinois, USA) and plotted using GraphPad Prism version 8 (GraphPad Prism Software Inc., San Diego, California, USA).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38572994", "locator": "sections[11] \"Statistics\" paragraph 2", "quote": "Statistical analyses were carried out using the ANOVA method followed by multiple comparisons corrected with Dunnett’s test in SPSS (version 26.0 for Windows, Chicago, Illinois, USA) and plotted using GraphPad Prism version 8 (GraphPad Prism Software Inc., San Diego, California, USA)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw MS data associated with this manuscript was submitted to the ProteomeXChange database and is available under the identifier PXD049077.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38572994", "locator": "sections[22] \"Data Availability Statement\" paragraph 2", "quote": "The raw MS data associated with this manuscript was submitted to the ProteomeXChange database and is available under the identifier PXD049077."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Thirteen immunoreactive spots common to sera from mice infected with both C. auris phenotypes corresponded to four proteins: enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate mutase.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38572994", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Regarding the immunoproteomics study, 13 spots recognized by sera from mice infected with both C. auris phenotypes and analyzed by mass spectrometry corresponded to enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate mutase."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Thirteen immunoreactive spots common to sera from mice infected with both C. auris phenotypes corresponded to four proteins: enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate mutase.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38572994"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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