Regulatory role of the miR-142-3p/CDC25C axis in modulating autophagy in non-small cell lung cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38601452", "kind": "science-record", "revision": 3, "content_hash": "sha256:54a0df5b9f862a5e1f777017d56e8bd3ad3a2ee5c9293880573fffef86700b48", "created_at": "2026-11-01T19:20:34Z", "updated_at": "2026-11-01T22:11:39Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Regulatory role of the miR-142-3p/CDC25C axis in modulating autophagy in non-small cell lung cancer.", "doi": "10.21037/tlcr-24-82", "pmid": "38601452", "authors": ["Jing Meng", "Zongchang Song", "Shuxian Cong", "Qiong Sun", "Qinyun Ma", "Weiwei Shi", "Linxuan Wang"], "journal": "Translational lung cancer research", "year": 2024, "abstract": "Background: With its diverse genetic foundation and heterogeneous nature, non-small cell lung cancer (NSCLC) needs a better comprehension of prognostic evaluation and efficient treatment targeting.\nMethods: Bioinformatics analysis was performed of The Cancer Genome Atlas (TCGA)-NSCLC and GSE68571 dataset. Overlapping differentially expressed genes (DEGs) were used for functional enrichment analysis and constructing the protein-protein interaction (PPI) network. In addition, key prognostic genes were identified through prognostic risk models, and their expression levels were verified. The phenotypic effects of cell division cycle 25C (CDC25C) regulation on NSCLC cell lines were assessed by in vitro experiments using various techniques such as flow cytometry, Transwell, and colony formation. Protein levels related to autophagy and apoptosis were assessed, specifically examining the impact of autophagy inhibition [3-methyladenine (3-MA)] and the miR-142-3p/CDC25C axis on this regulatory system.\nResults: CDC25C was identified as a key prognostic marker in NSCLC, showing high expression in tumor samples. In vitro experiments showed that CDC25C knockdown markedly reduced the capacity of cells to proliferate, migrate, invade, trigger apoptosis, and initiate cell cycle arrest. CDC25C and miR-142-3p displayed a reciprocal regulatory relationship. CDC25C reversed the inhibitory impacts of miR-142-3p on NSCLC cell cycle proliferation and progression. The synergy of miR-142-3p inhibition, CDC25C silencing, and 3-MA treatment was shown to regulate NSCLC cell processes including proliferation, apoptosis, and autophagy.\nConclusions: MiR-142-3p emerged as a key player in governing autophagy and apoptosis by directly targeting CDC25C expression. This emphasizes the pivotal role of the miR-142-3p/CDC25C axis as a critical regulatory pathway in NSCLC.", "claims": [{"id": "claim-method", "statement": "We used LASSO Cox regression analysis on 42 genes in the PPI network to look for possible predictive biomarkers.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38601452", "locator": "sections[20] \"Identification of seven signature prognostic genes associated with NSCLC\" paragraph 2", "quote": "We used LASSO Cox regression analysis on 42 genes in the PPI network to look for possible predictive biomarkers."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data Sharing Statement: Available at https://tlcr.amegroups.com/article/view/10.21037/tlcr-24-82/dss", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38601452", "locator": "sections[33] \"Footnotes\" paragraph 2", "quote": "Data Sharing Statement: Available at https://tlcr.amegroups.com/article/view/10.21037/tlcr-24-82/dss"}], "state": "complete"}, {"id": "claim-target-result", "statement": "CDC25C was identified as a key prognostic marker in NSCLC, showing high expression in tumor samples.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38601452", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "CDC25C was identified as a key prognostic marker in NSCLC, showing high expression in tumor samples."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "CDC25C was identified as a key prognostic marker in NSCLC, showing high expression in tumor samples.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38601452"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Thi"], "order_basis": "paper_text_order:897", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["les. 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