Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38714919", "kind": "science-record", "revision": 2, "content_hash": "sha256:e35b764aa4b526e93917e802a35b352987f8c1f7d1110a401ffbfc0f925e6d20", "created_at": "2026-11-01T19:20:40Z", "updated_at": "2026-11-01T21:33:04Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon.", "doi": "10.1186/s12864-024-10309-y", "pmid": "38714919", "authors": ["Natalia Kodzik", "Andrzej Ciereszko", "Bożena Szczepkowska", "Agata Malinowska", "Mariola Aleksandra Dietrich"], "journal": "BMC genomics", "year": 2024, "abstract": "BACKGROUND: Sturgeon species are living fossils that exhibit unique reproductive characteristics, and elucidation of the molecular processes governing the formation and quality of sturgeon eggs is crucial. However, comprehensive data on the protein composition of sturgeon ovarian fluid (OF) and eggs and their functional significance are lacking. To address this knowledge gap, the aim of the present study was to conduct a comprehensive comparative proteomic analysis of Siberian sturgeon OF and eggs using liquid chromatography-mass spectrometry (LC-MS/MS).\nRESULTS: A total of 617 proteins were identified in OF, and 565 proteins were identified in eggs. A total of 772 proteins showed differential abundance. Among the differentially abundant proteins, 365 were more abundant in OFs, while 407 were more abundant in eggs. We identified 339 proteins unique to OFs and 287 proteins specific to eggs, and further investigated the top 10 most abundant proteins in each. The functional annotation of the OF proteins highlighted their predominant association with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of the actin cytoskeleton. Analysis of egg proteins revealed enrichment in metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, and protein ubiquitination and translation. OF-specific proteins included extracellular matrix and secretory vesicles, and eggs were enriched in proteins localized to mitochondria and ribosome components.\nCONCLUSIONS: This study presents the first comprehensive characterization of the protein composition of sturgeon OF and eggs and elucidates their distinct functional roles. These findings advance our understanding of sturgeon reproduction, OF-egg signaling and the origin of OF proteins. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier PXD044168 to ensure accessibility for further research.", "claims": [{"id": "claim-method", "statement": "The FDR was calculated with the target/decoy strategy and kept under 1%.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38714919", "locator": "sections[28] \"Data analysis\" paragraph 2", "quote": "The FDR was calculated with the target/decoy strategy and kept under 1%."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE [71] partner repository with the dataset identifiers PXD044168 and 10.6019/PXD044168.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38714919", "locator": "sections[38] \"notes\" paragraph 1", "quote": "The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE [71] partner repository with the dataset identifiers PXD044168 and 10.6019/PXD044168."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using the emPAI-based estimates of the quantitative relationships within each type of sample, a total of 772 differentially abundant proteins (DAPs) between the OF and eggs were identified, including 365 proteins that were more abundant in the OF and 407 proteins that were more abundant in eggs (Supplementary Table S5).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38714919", "locator": "sections[6] \"Comparison of OF and egg proteins\" paragraph 2", "quote": "Using the emPAI-based estimates of the quantitative relationships within each type of sample, a total of 772 differentially abundant proteins (DAPs) between the OF and eggs were identified, including 365 proteins that were more abundant in the OF and 407 proteins that were more abundant in eggs (Supplementary Table S5)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Using the emPAI-based estimates of the quantitative relationships within each type of sample, a total of 772 differentially abundant proteins (DAPs) between the OF and eggs were identified, including 365 proteins that were more abundant in the OF and 407 proteins that were more abundant in eggs (Supplementary Table S5).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38714919"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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