25-hydroxycholesterol aggravates oxygen-glucose deprivation/reoxygenation-induced pyroptosis through promoting activation of NLRP3 inflammasome in H9C2 cardiomyocytes.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38716981", "kind": "science-record", "revision": 5, "content_hash": "sha256:bb26709e2adb98b45a9081220a95c313540f2f99b20460c01e34f45e09ee5b67", "created_at": "2026-11-01T19:20:41Z", "updated_at": "2026-11-01T22:54:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "25-hydroxycholesterol aggravates oxygen-glucose deprivation/reoxygenation-induced pyroptosis through promoting activation of NLRP3 inflammasome in H9C2 cardiomyocytes.", "doi": "10.1590/1414-431X2024e13299", "pmid": "38716981", "authors": ["Tao Jiang", "Yong Li"], "journal": "Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica", "year": 2024, "abstract": "25-hydroxycholesterol (25-HC) plays a role in the regulation of cell survival and immunity. However, the effect of 25-HC on myocardial ischemia/reperfusion (MI/R) injury remains unknown. Our present study aimed to investigate whether 25-HC aggravated MI/R injury through NLRP3 inflammasome-mediated pyroptosis. The overlapping differentially expressed genes (DEGs) in MI/R were identified from the GSE775, GSE45818, GSE58486, and GSE46395 datasets in Gene Expression Omnibus (GEO) database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted using the database of Annotation, Visualization and Integration Discovery (DAVID). The protein-protein interaction (PPI) network of the overlapping DEGs was established using the Search Tool for the Retrieval of Interacting Genes (STRING) database. These bioinformatics analyses indicated that cholesterol 25-hydroxylase (CH25H) was one of the crucial genes in MI/R injury. The oxygen-glucose deprivation/reoxygenation (OGD/R) cell model was established to simulate MI/R injury. Western blot and RT-qPCR analysis demonstrated that CH25H was significantly upregulated in OGD/R-stimulated H9C2 cardiomyocytes. Moreover, knockdown of CH25H inhibited the OGD/R-induced pyroptosis and nod-like receptor protein 3 (NLRP3) inflammasome activation, as demonstrated by cell counting kit-8 (CCK8), lactate dehydrogenase (LDH), RT-qPCR, and western blotting assays. Conversely, 25-HC, which is synthesized by CH25H, promoted activation of NLRP3 inflammasome in OGD/R-stimulated H9C2 cardiomyocytes. In addition, the NLRP3 inhibitor BAY11-7082 attenuated 25-HC-induced H9C2 cell injury and pyroptosis under OGD/R condition. In conclusion, 25-HC could aggravate OGD/R-induced pyroptosis through promoting activation of NLRP3 inflammasome in H9C2 cells.", "claims": [{"id": "claim-method", "statement": "The results were analyzed using one-way ANOVA with Tukey multiple comparison test in GraphPad Prism version 5.0 (USA).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38716981", "locator": "sections[15] \"Statistical analysis\" paragraph 2", "quote": "The results were analyzed using one-way ANOVA with Tukey multiple comparison test in GraphPad Prism version 5.0 (USA)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38716981", "locator": "sections[3] \"Microarray datasets\" paragraph 2", "quote": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In conclusion, 25-HC could aggravate OGD/R-induced pyroptosis through promoting activation of NLRP3 inflammasome in H9C2 cells.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38716981", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In conclusion, 25-HC could aggravate OGD/R-induced pyroptosis through promoting activation of NLRP3 inflammasome in H9C2 cells."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "In conclusion, 25-HC could aggravate OGD/R-induced pyroptosis through promoting activation of NLRP3 inflammasome in H9C2 cells.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38716981"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE45818"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE45818"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:38716981", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1590/1414-431X2024e13299", "sha256": "sha256:be304cdb72511bfb14775d9b02396580c3730ba0df4438722235fa1f7847d37e", "bytes": 27041, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1590/1414-431X2024e13299", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC11085030", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE45818", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE45818", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE45818", "version": null, "note": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM1115938", "GSM1115939", "GSM1115940", "GSM1115941", "GSM1115942", "GSM1115943", "GSM1115944", "GSM1115945", "GSM1115946", "GSM1115947", "GSM1115948", "GSM1115949", "GSM1115950", "GSM1115951", "GSM1115952", "GSM1115953", "GSM1115954", "GSM1115955", "GSM1115956", "GSM1115957", "GSM1115958", "GSM1115959", "GSM1115960", "GSM1115961"], "sample_count": 24, "group_sizes": null, "group_sizes_basis": null, "platform": "11533", "taxon": "Mus musculus", "title": "Differential Gene Regulation during murine in vivo heart ischemia comparing wildtype and Per2 deficient mice", "resolution_checked_at": "2026-11-01T20:16:08Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE46395", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46395", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE46395", "version": null, "note": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM1129735", "GSM1129736", "GSM1129737", "GSM1129738", "GSM1129739", "GSM1129740", "GSM1129741", "GSM1129742"], "sample_count": 8, "group_sizes": null, "group_sizes_basis": null, "platform": "10787", "taxon": "Mus musculus", "title": "Gene expression in the heart of mouse with myocardial infarction", "resolution_checked_at": "2026-11-01T20:16:08Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE58486", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE58486", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE58486", "version": null, "note": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM1412423", "GSM1412424", "GSM1412425", "GSM1412426", "GSM1412427", "GSM1412428", "GSM1412429", "GSM1412430", "GSM1412431", "GSM1412432", "GSM1412433", "GSM1412434", "GSM1412435", "GSM1412436", "GSM1412437", "GSM1412438", "GSM1412439", "GSM1412440"], "sample_count": 18, "group_sizes": null, "group_sizes_basis": null, "platform": "18802", "taxon": "Mus musculus", "title": "CaM Kinase II mediates maladaptive post-infarct remodeling but not acute myocardial ischemia/reperfusion injury", "resolution_checked_at": "2026-11-01T20:16:08Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE775", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE775", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE775", "version": null, "note": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14).", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM12322", "GSM12323", "GSM12324", "GSM12325", "GSM12326", "GSM12327", "GSM12328", "GSM12329", "GSM12330", "GSM12331", "GSM12332", "GSM12333", "GSM12334", "GSM12335", "GSM12336", "GSM12337", "GSM12338", "GSM12339", "GSM12340", "GSM12341", "GSM12342", "GSM12343", "GSM12344", "GSM12345", "GSM12346", "GSM12347", "GSM12348", "GSM12349", "GSM12350", "GSM12351", "GSM12352", "GSM12353", "GSM12354", "GSM12355", "GSM12356", "GSM12357", "GSM12358", "GSM12359", "GSM12360", "GSM12361", "GSM12362", "GSM12363", "GSM12364", "GSM12365", "GSM12366", "GSM12367", "GSM12368", "GSM12369", "GSM12370", "GSM12371", "GSM12372", "GSM12373", "GSM12374", "GSM12375", "GSM12376", "GSM12377", "GSM12378", "GSM12379", "GSM12380"], "sample_count": 59, "group_sizes": null, "group_sizes_basis": null, "platform": "81", "taxon": "Mus musculus", "title": "Mouse model of myocardial infarction", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE45818 not downloaded", "asset:data:geo:GSE45818 licence not read", "asset:data:geo:GSE46395 not downloaded", "asset:data:geo:GSE46395 licence not read", "asset:data:geo:GSE58486 not downloaded", "asset:data:geo:GSE58486 licence not read", "asset:data:geo:GSE775 not downloaded", "asset:data:geo:GSE775 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "The results were analyzed using one-way ANOVA with Tukey multiple comparison test in GraphPad Prism version 5.0 (USA)."}}, {"id": "method-2", "family": "network_analysis", "specific": "ppi_network", "label": "Network / pathway analysis", "dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.7, "band": "medium", "quote": "protein-protein interaction"}}, {"id": "method-3", "family": "network_analysis", "specific": "pathway_enrichment", "label": "Network / pathway analysis", "dsl_operations": ["pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.7, "band": "medium", "quote": "pathway enrichment"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: anova."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE775", "GSE45818", "GSE58486", "GSE46395"], "resolved_accessions": ["GSE775", "GSE45818", "GSE58486", "GSE46395"], "statements": ["The datasets GSE775, GSE45818, GSE58486, and GSE46395 were obtained from the Gene Expression Omnibus (GEO) database. C57BL/6 adult male mice (11-12 weeks old) were used in GSE775 and the 12-to-16-week-old male mice were used in GSE45818 (13,14)."], "basis": "The paper names GSE775, GSE45818, GSE58486, GSE46395 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "assets": ["source:pmid:38716981", "data:geo:GSE45818"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE45818"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T19:17:54Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": null}, "calls": 5, "usage": {"prompt_tokens": 8935, "completion_tokens": 1558, "cached_tokens": 128}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:38716981", "type": "paper", "label": "pmid:38716981"}, {"id": "source:pmid:38716981", "type": "manuscript", "label": "PMID 38716981 full text"}, {"id": "data:geo:GSE45818", "type": "dataset", "label": "GEO GSE45818"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:38716981", "type": "paper", "label": "pmid:38716981"}, {"id": "source:pmid:38716981", "type": "manuscript", "label": "PMID 38716981 full text"}, {"id": "data:geo:GSE45818", "type": "dataset", "label": "GEO GSE45818"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-38716981", "type": "extraction", "used": ["source:pmid:38716981"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE45818", "type": "accession_resolution", "used": ["data:geo:GSE45818"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:38716981", "parent_hash": "sha256:a54dcac9bdd48ce3758696f3bf3227b512b5ed2ae2b2ed34a9dbfffd6a315601", "fork_of": null, "generation": 4}, "gaps": ["asset:data:geo:GSE45818 licence not read", "asset:data:geo:GSE45818 not downloaded", "asset:data:geo:GSE46395 licence not read", "asset:data:geo:GSE46395 not downloaded", "asset:data:geo:GSE58486 licence not read", "asset:data:geo:GSE58486 not downloaded", "asset:data:geo:GSE775 licence not read", "asset:data:geo:GSE775 not downloaded", "execution.not_run", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["del was established to simulate MI/R injury. Western blot and RT-qPCR analysis demonstrated that CH25H", "), lactate dehydrogenase (LDH), RT-qPCR, and western blotting assays. Conversely, 25-HC, which is synthesi"], "order_basis": "paper_text_order:1206", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ed to simulate MI/R injury. Western blot and RT-qPCR analysis demonstrated that CH25H was signifi", "g kit-8 (CCK8), lactate dehydrogenase (LDH), RT-qPCR, and western blotting assays. Conversely, 25", "to simulate MI/R injury. Western blot and RT-qPCR analysis demonstrated that CH25H was signifi", "it-8 (CCK8), lactate dehydrogenase (LDH), RT-qPCR, and western blotting assays. Conversely, 25"], "order_basis": "paper_text_order:1223", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["/R-stimulated H9C2 cardiomyocytes. Moreover, knockdown of CH25H inhibited the OGD/R-induced pyropto"], "order_basis": "paper_text_order:1345", "id": "assay:3"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ion, as demonstrated by cell counting kit-8 (CCK8), lactate dehydrogenase (LDH), RT-qPCR, and"], "order_basis": "paper_text_order:1508", "id": "assay:4"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["replaced every two to three days. Cells were cultured in a humidified 5% CO2 incubator (ESCO, Singapo"], "order_basis": "paper_text_order:2400", "id": "assay:5"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The results were analyzed using one-way ANOVA with Tukey multiple comparison test in GraphPad Prism version 5.0 (USA)."], "order_basis": "consumes_earlier_output", "id": "assay:6"}]}