Preliminary Analysis of Aging-Related Genes in Intracerebral Hemorrhage by Integration of Bulk and Single-Cell RNA Sequencing Technology.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38883702", "kind": "science-record", "revision": 4, "content_hash": "sha256:13d2cc19f27bac7297f867cf0f9d9392005a5bb238500f5fe2c7ae48084b5608", "created_at": "2026-11-01T20:28:57Z", "updated_at": "2026-11-01T22:54:30Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Preliminary Analysis of Aging-Related Genes in Intracerebral Hemorrhage by Integration of Bulk and Single-Cell RNA Sequencing Technology.", "doi": "10.2147/IJGM.S457480", "pmid": "38883702", "authors": ["Qianfeng Li", "Bo Wang", "Jun Yang", "Yuan Wang", "Faliang Duan", "Ming Luo", "Chungang Zhao", "Wei Wei", "Lei Wang", "Sha Liu"], "journal": "International journal of general medicine", "year": 2024, "abstract": "Background: Aging is recognized as the key risk for intracerebral hemorrhage (ICH). The detailed mechanisms of aging in ICH warrant exploration. This study aimed to identify potential aging-related genes associated with ICH.\nMethods: ICH-specific aging-related genes were determined by the intersection of differentially expressed genes (DEGs) between perihematomal tissues and corresponding contralateral parts of four patients with ICH (GSE24265) and 349 aging-related genes obtained from the Aging Atlas database. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) analyses were performed to identify the potential biological functions and pathways in which these ICH-specific aging-related genes may be involved. Then, PPI network was established to identify the hub genes of ICH-specific aging-related genes. Meanwhile, miRNA-mRNA and transcription factor (TF)-mRNA regulatory networks were constructed to further explore the ICH-specific aging-related genes regulation. The relationship between these hub genes and immune infiltration was also further explored. Additional single-cell RNA-seq analysis (scRNA-seq, GSE167593) was used to locate the hub genes in different cell types. Besides, expression levels of the hub genes were validated using clinical samples from our institute and another GEO dataset (GSE206971).\nResults: This study identified 24 ICH-specific aging-related genes, including 22 up-regulated and 2 down-regulated genes. The results of GO and KEGG suggested that the ICH-specific aging-related genes mainly enriched in immunity and inflammation-related pathways, suggesting that aging may affect the ich pathogenesis by regulating inflammatory and immune-related pathways.\nConclusion: Our study revealed 24 ICH-specific aging-related genes and their functions highly pertinent to ICH pathogenesis, providing new insights into the impact of aging on ICH.", "claims": [{"id": "claim-method", "statement": "The Limma package30 was used to perform the analysis of differential expression.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38883702", "locator": "sections[4] \"Different Expression Analysis\" paragraph 2", "quote": "The Limma package30 was used to perform the analysis of differential expression."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The gene expression profiles used in this study are available in the Gene Expression Omnibus (GSE24265, GEO Accession viewer (nih.gov); GSE167593, GEO Accession viewer (nih.gov); GSE206971, GEO Accession viewer (nih.gov)). The results generated during the present study are available from the corresponding author on request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38883702", "locator": "sections[25] \"Data Sharing Statement\" paragraph 2", "quote": "The gene expression profiles used in this study are available in the Gene Expression Omnibus (GSE24265, GEO Accession viewer (nih.gov); GSE167593, GEO Accession viewer (nih.gov); GSE206971, GEO Accession viewer (nih.gov)). The results generated during the present study are available from the corresponding author on request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "This study identified 24 ICH-specific aging-related genes, including 22 up-regulated and 2 down-regulated genes.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38883702", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "This study identified 24 ICH-specific aging-related genes, including 22 up-regulated and 2 down-regulated genes."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "This study identified 24 ICH-specific aging-related genes, including 22 up-regulated and 2 down-regulated genes.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38883702"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE167593"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE167593"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:38883702", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/IJGM.S457480", "sha256": "sha256:d07d93299da069b3e5c9dc3af1aee3defe6dfa1b477b30a6a9731847f432ccd8", "bytes": 38742, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.2147/IJGM.S457480", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC11180471", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE167593", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE167593", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE167593", "version": null, "note": "The gene expression profiles used in this study are available in the Gene Expression Omnibus (GSE24265, GEO Accession viewer (nih.gov); GSE167593, GEO Accession viewer (nih.gov); GSE206971, GEO Accession viewer (nih.gov)). 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