Identification of potential crucial cuproptosis-related genes in myocardial ischemia-reperfusion injury through the bioinformatic analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38901133", "kind": "science-record", "revision": 3, "content_hash": "sha256:8a0d183c079dc12e82da07797fc3ed11b11bf3189259cbf34a4b0839d66db78d", "created_at": "2026-11-01T20:28:58Z", "updated_at": "2026-11-01T22:12:05Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of potential crucial cuproptosis-related genes in myocardial ischemia-reperfusion injury through the bioinformatic analysis.", "doi": "10.1016/j.clinsp.2024.100410", "pmid": "38901133", "authors": ["Rong Huang", "Rongfeng Xu", "Rui Zhang", "Wenjie Zuo", "Zhenjun Ji", "Zaixiao Tao", "Yongjun Li", "Genshan Ma"], "journal": "Clinics (Sao Paulo, Brazil)", "year": 2024, "abstract": "BACKGROUND: Cuproptosis is known to regulate diverse physiological functions in many diseases, but its role in regulating Myocardial Ischemia-Reperfusion Injury (MI/RI) remains unclear.\nMETHODS: For this purpose, the MI/RI microarray datasets GSE61592 were downloaded from the Gene Expression Omnibus (GEO) database, and the Differently Expressed Genes (DEGs) in MI/RI were identified using R software. Moreover, the MI/RI mice model was established to confirm further the diagnostic value of Pyruvate Dehydrogenase B (Pdhb), Dihydrolipoamide S-acetyltransferase (Dlat), and Pyruvate dehydrogenase E1 subunit alpha 1 (Pdhα1).\nRESULTS: The analysis of microarray datasets GSE61592 revealed that 798 genes were upregulated and 768 were downregulated in the myocardial tissue of the ischemia-reperfusion injury mice. Furthermore, Dlat, Pdhb, Pdhα1, and cuproptosis-related genes belonged to the downregulated genes. The receiver operating characteristics curve analysis results indicated that the Dlat, Pdhb, and Pdhα1 levels were downregulated in MI/RI and were found to be potential biomarkers for MI/RI diagnosis and prognosis. Similarly, analysis of Dlat, Pdhb, and Pdhα1 levels in the MI/RI mice revealed Pdhb being the key diagnostic marker.\nCONCLUSIONS: This study demonstrated the prognostic value of cuproptosis-related genes (Dlat, Pdhb, and Pdhα1), especially Pdhb, MI/RI, providing new insight into the MI/RI treatment.", "claims": [{"id": "claim-method", "statement": "Receiver Operating Characteristics (ROC) curve analysis was conducted to predict the diagnostic value of Pdhb, Dlat, and Pdhα1.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38901133", "locator": "sections[10] \"Statistical analysis\" paragraph 2", "quote": "Receiver Operating Characteristics (ROC) curve analysis was conducted to predict the diagnostic value of Pdhb, Dlat, and Pdhα1."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The gene expression profiles GSE61592, containing microarrays of cardiac tissue samples from a mice model of MI/RI and normal mice cardiac tissue samples, were obtained from the Gene Expression Omnibus (GEO) database, using GPL96 platform for acquiring profile data.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38901133", "locator": "sections[4] \"Identificcation of DEGs\" paragraph 2", "quote": "The gene expression profiles GSE61592, containing microarrays of cardiac tissue samples from a mice model of MI/RI and normal mice cardiac tissue samples, were obtained from the Gene Expression Omnibus (GEO) database, using GPL96 platform for acquiring profile data."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Pdhb was the key diagnostic marker for myocardial ischemia-reperfusion injury.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:38901133", "locator": "sections[0] \"Abstract\" paragraph 8", "quote": "Similarly, analysis of Dlat, Pdhb, and Pdhα1 levels in the MI/RI mice revealed Pdhb being the key diagnostic marker."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Pdhb was the key diagnostic marker for myocardial ischemia-reperfusion injury.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:38901133"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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