Development and validation of a novel disulfidptosis-related lncRNAs signature in patients with HPV-negative oral squamous cell carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:38910181", "kind": "science-record", "revision": 3, "content_hash": "sha256:0f77ac8efb5348819407a5be677e15ff2ecad9c061ae174f4936fe7ab32e95aa", "created_at": "2026-11-01T20:28:58Z", "updated_at": "2026-11-01T22:12:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Development and validation of a novel disulfidptosis-related lncRNAs signature in patients with HPV-negative oral squamous cell carcinoma.", "doi": "10.1038/s41598-024-65194-y", "pmid": "38910181", "authors": ["Fan Yang", "Xinyu Niu", "Mingzhu Zhou", "Wei Li"], "journal": "Scientific reports", "year": 2024, "abstract": "Disulfidptosis is a recently identified mode of regulated cell death. Regulating disulfidptosis in carcinoma is a promising therapeutic approach. Long non-coding RNAs (lncRNAs) have been reported to be related to the occurrence and development of many cancers. Disulfidptosis-related lncRNAs (DRLs) in HPV-negative oral squamous cell carcinoma (OSCC) have not been studied. Based on The Cancer Genome Atlas (TCGA) database, least absolute shrinkage selection operator (LASSO) analysis and Cox regression analysis were used to identify overall survival related DRLs and construct the signature. Kaplan-Meier, time-dependent receiver operating characteristics (ROC) and principal component analyses (PCA) were explored to demonstrate the prediction potential of the signature. Subgroup analysis stratified by different clinicopathological characteristics were conducted. Nomogram was established by DRLs signature and independent clinicopathological characteristics. The calibration plots were performed to reveal the accuracy of nomogram. Immune cell subset infiltration, immunotherapy response, drug sensitivity analysis, and tumor mutation burden (TMB) were conducted. Underlying functions and pathways were explored by Gene Set Enrichment Analysis (GSEA) analysis. Previous lncRNA signatures of OSCC were retrieved from PubMed for further validation. Gene expression omnibus (GEO) datasets (GSE41613 and GSE85446) were merged as an external validation for DRLs signature. Consensus clustering analysis of DRLs signature and experimental validation of DRLs were also explored. This research sheds light on the robust performance of DRLs signature in survival prediction, immune cell infiltration, immune escape, and immunotherapy of HPV-negative OSCC.", "claims": [{"id": "claim-method", "statement": "LASSO regression analysis was stepwise applied to the training set. Three candidate DRLs of LASSO regression analysis were further screen by multivariate Cox regression analysis (Fig. 2C,D).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38910181", "locator": "sections[21] \"Identification of DRLs signature\" paragraph 2", "quote": "LASSO regression analysis was stepwise applied to the training set. Three candidate DRLs of LASSO regression analysis were further screen by multivariate Cox regression analysis (Fig. 2C,D)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in this article. Further inquiries directed to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38910181", "locator": "sections[38] \"notes\" paragraph 1", "quote": "The original contributions presented in the study are included in this article. 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The up-regulated expressio", "E expression level in SCC-9 cells after 48 h transfection. (C) Cell viability between vector and AC104", ". (D) Representative images of AC104794.3-OE transfection in the cell proliferation of SCC-9 cells usi", "sentative images of migration of SCC-9 cells transfected with AC104794.3-OE. Scale bar, 200 μm. *P <"], "order_basis": "paper_text_order:6504", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["luated by qPT-PCR (Fig. 11B). The results of CCK-8 assays revealed that over-expression lncRNA", "3-OE groups in SCC-9 cells was determined by CCK-8 assay. 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