The quantification of zebrafish ocular-associated proteins provides hints for sex-biased visual impairments and perception.

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Abundance differences of eye proteins could provide explanations for some of these. Exploration of the ocular proteome was performed to find sex-based protein abundance differences in zebrafish Danio rerio. A label-free protein quantification workflow using high-resolution mass spectrometry was employed to find proteins with significant differences between the sexes. In total, 3740 unique master proteins were identified and quantified, and 49 proteins showed significant abundance differences between the eyes of male and female zebrafish. Those proteins belong to lipoproteins, immune system, blood coagulation, antioxidants, iron and heme-binding proteins, ion channels, pumps and exchangers, neuronal and photoreceptor proteins, and the cytoskeleton. An extensive literature review provided clues for the possible links between the sex-biased level of proteins and visual perception and impairments. In conclusion, sexual dimorphism at the protein level was discovered for the first time in the eye of zebrafish and should be accounted for in ophthalmological studies. Data are available via ProteomeXchange with identifier PXD033338.", "claims": [{"id": "claim-method", "statement": "The protein-level abundance data were subjected to empirical Bayes t-test (Limma) [21] between samples of the two sexes in NormalyzerDE, with Benjamini-Hochberg adjustment of p-values for FDR filtering, with the adjusted p-values referred to as q-values hereafter.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:38994070", "locator": "sections[6] \"Data processing and analysis\" paragraph 3", "quote": "The protein-level abundance data were subjected to empirical Bayes t-test (Limma) [21] between samples of the two sexes in NormalyzerDE, with Benjamini-Hochberg adjustment of p-values for FDR filtering, with the adjusted p-values referred to as q-values hereafter."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [96] partner repository with the dataset identifier PXD033338. 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