Investigation into the role of H2-Ab1 in vascular remodeling in pulmonary arterial hypertension via Bioinformatics.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39010027", "kind": "science-record", "revision": 2, "content_hash": "sha256:a0c082532274d0d4ead092aa8fd9eacc36c63e945b00ece1e6355424224fc808", "created_at": "2026-11-01T20:29:05Z", "updated_at": "2026-11-01T21:33:28Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Investigation into the role of H2-Ab1 in vascular remodeling in pulmonary arterial hypertension via Bioinformatics.", "doi": "10.1186/s12890-024-03156-w", "pmid": "39010027", "authors": ["Guowen Wang", "Zhuoyan Wang"], "journal": "BMC pulmonary medicine", "year": 2024, "abstract": "BACKGROUND: Pulmonary arterial hypertension (PAH) is a progressive disease of vascular remodeling characterized by persistent pulmonary arterial pressure elevation, which can lead to right heart failure and premature death. Given the complex pathogenesis and poor prognosis of PAH, the identification and investigation of biomarkers become increasingly critical for advancing further understanding of the disease.\nMETHODS: PAH-related datasets, GSE49114, GSE180169 and GSE154959, were downloaded from the publicly available GEO database. By performing WGCNA on the GSE49114 dataset, a total of 906 PAH-related key module genes were screened out. By carrying out differential analysis on the GSE180169 dataset, a total of 576 differentially expressed genes were identified. Additionally, the GSE154959 single-cell sequencing dataset was also subjected to differential analysis, leading to the identification of 34 DEGs within endothelial cells. By taking intersection of the above three groups of DEGs, five PAH-related hub genes were screened out, namely Plvap, Cyp4b1, Foxf1, H2-Ab1, and H2-Eb1, among which H2-Ab1 was selected for subsequent experiments.\nRESULTS: A SuHx mouse model was prepared using the SU5416/hypoxia method, and the successful construction of the model was evaluated through Hematoxylin-Eosin staining, hemodynamic detection, fulton index, and Western Blot (WB). The results of WB and qRT-PCR demonstrated a significant upregulation of H2-Ab1 expression in SuHx mice. Consistent with the results of bioinformatics analysis, a time-dependent increase was observed in H2-Ab1 expression in hypoxia-treated mouse pulmonary artery endothelial cells (PAECs). To investigate whether H2-Ab1 affects the development and progression of PAH, we knocked down H2-Ab1 expression in PAECs, and found that its knockdown inhibited the viability, adhesion, migration, and angiogenesis, while concurrently promoted the apoptosis of PAECs.\nCONCLUSION: H2-Ab1 could regulate the proliferation, apoptosis, adhesion, migration, and angiogenesis of PAECs.", "claims": [{"id": "claim-method", "statement": "Measurement data were expressed as mean ± standard deviation (x ± s), and t-test was used to assess sample differences between two groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39010027", "locator": "sections[19] \"Statistical analysis\" paragraph 2", "quote": "Measurement data were expressed as mean ± standard deviation (x ± s), and t-test was used to assess sample differences between two groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and/or analysed during the current study are available in the GEO database (https://www.ncbi.nlm.nih.gov/geo/) (accession numbers: GSE49114, GSE180169 and GSE154959).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39010027", "locator": "sections[36] \"notes\" paragraph 1", "quote": "The datasets generated and/or analysed during the current study are available in the GEO database (https://www.ncbi.nlm.nih.gov/geo/) (accession numbers: GSE49114, GSE180169 and GSE154959)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39010027"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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RESULTS: A SuHx mouse model was prepared using the SU5416/hypoxia method", "for subsequent experiments. RESULTS: A SuHx mouse model was prepared using the SU5416/hypoxia", "rved in H2-Ab1 expression in hypoxia-treated mouse pulmonary artery endothelial cells (PAECs).", "edical College Laboratory Animal Center. The mice were kept under constant temperature and humidity"], "order_basis": "paper_text_order:1245", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ng, hemodynamic detection, fulton index, and Western Blot (WB). The results of WB and qRT-PCR demonstr"], "order_basis": "paper_text_order:1438", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["-Ab1 expression in PAECs, and found that its knockdown inhibited the viability, adhesion, migration"], "order_basis": "paper_text_order:1888", "id": "assay:3"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ngiogenesis, while concurrently promoted the apoptosis of PAECs. CONCLUSION: H2-Ab1 could regulate", "ON: H2-Ab1 could regulate the proliferation, apoptosis, adhesion, migration, and angiogenesis of PA"], "order_basis": "paper_text_order:1994", "id": "assay:4"}]}