Extracellular Matrix Protein Signatures of the Outer and Inner Zones of the Rat Adrenal Cortex.

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Proteomic profiles of decellularized OF and IF samples, male and female rats, identified 252 proteins, with 32 classified as ECM-component and ECM-related. Among these, 25 proteins were differentially regulated: 17 more abundant in OF, including Col1a1, Col1a2, Col6a1, Col6a2, Col6a3, Col12a1, Col14a1, Lama5, Lamb2, Lamc1, Eln, Emilin, Fbln5, Fbn1, Fbn2, Nid1, and Ltbp4, and eight more abundant in IF, including Col4a1, Col4a2, Lama2, Lama4, Lamb1, Fn1, Hspg2, and Ecm1. Eln, Tnc, and Nid2 were abundant in the female OF, while Lama2, Lama5, Lamb2, and Lamc1 were more abundant in the male IF. The complex protein signature of the OF suggests areas of tissue stress, stiffness, and regulatory proteins for growth factor signaling. The higher concentrations of Col4a1 and Col4a2 and their role in steroidogenesis should be further investigated in IF. These findings could significantly enhance our understanding of adrenal cortex functionality and its implications for human health and disease. Key findings were validated, and data are available in ProteomeXchange (PXD046828).", "claims": [{"id": "claim-method", "statement": "A Student’s t test (p < 0.05) was applied to identify the significant proteins between OFs and IFs.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39018382", "locator": "sections[8] \"Data and Bioinformatics Analysis\" paragraph 2", "quote": "A Student’s t test (p < 0.05) was applied to identify the significant proteins between OFs and IFs."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Key findings were validated, and data are available in ProteomeXchange (PXD046828).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39018382", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Key findings were validated, and data are available in ProteomeXchange (PXD046828)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Among 32 ECM-component and ECM-related proteins, 25 were differentially regulated between outer and inner adrenal fractions, with 17 more abundant in OF and eight more abundant in IF.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39018382", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Among these, 25 proteins were differentially regulated: 17 more abundant in OF, including Col1a1, Col1a2, Col6a1, Col6a2, Col6a3, Col12a1, Col14a1, Lama5, Lamb2, Lamc1, Eln, Emilin, Fbln5, Fbn1, Fbn2, Nid1, and Ltbp4, and eight more abundant in IF, including Col4a1, Col4a2, Lama2, Lama4, Lamb1, Fn1, Hspg2, and Ecm1."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Among 32 ECM-component and ECM-related proteins, 25 were differentially regulated between outer and inner adrenal fractions, with 17 more abundant in OF and eight more abundant in IF.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39018382"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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