Prognostic analysis of anoikis-related genes in bladder cancer: An observational study.

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However, the impact of anoikis on the prognosis of bladder cancer (BLCA) is currently unknown. Thus, this study aimed to find potential effect of anoikis in BLCA. The Cancer Genome Atlas (TCGA)-BLCA and GSE13507 cohorts were downloaded from TCGA and the Gene Expression Omnibus (GEO) databases, respectively. Differentially expressed genes (DEGs) were screened between BLCA and normal groups, which intersected with anoikis-related genes to yield anoikis-related DEGs (AR DEGs). Univariate COX, rbsurv, and multivariate COX analyses were adopted in order to build a prognostic risk model. The differences of risk score in the different clinical subgroups and the relevance between survival rate and clinical characteristics were explored as well. Finally, chemotherapy drug sensitivity in different risk groups was analyzed. In total, 78 AR DEGs were acquired and a prognostic signature was build based on the 6 characteristic genes (CALR, FASN, CSPG4, HGF, INHBB, SATB1), where the patients of low-risk group had longer survival time. The survival rate of BLCA patients was significantly differential in different groups of age, stage, smoking history, pathologic-T, and pathologic-N. The IC50 of 56 drugs showed significant differences between 2 risk groups, such as imatinib, docetaxel, and dasatinib. At last, the results of real time quantitative-polymerase chain reaction (RT-qPCR) demonstrated that the expression trend of CALR, HGF, and INHBB was consistent with the result obtained previously based on public databases. Taken together, this study identified 6 anoikis-related characteristic genes (CALR, FASN, CSPG4, HGF, INHBB, SATB1) for the prognosis of BLCA patients, providing a scientific reference for further research on BLCA.", "claims": [{"id": "claim-method", "statement": "Additionally, we also analyzed DEGs 2 between BLCA and normal groups in TCGA-BLCA dataset via “DEseq2” R package (version 1.36.0) with |log2FC| > 1 and adj P < .05.[12]", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39029056", "locator": "sections[4] \"2.2. Identification of differentially expressed genes (DEGs) between BLCA and normal groups\" paragraph 2", "quote": "Additionally, we also analyzed DEGs 2 between BLCA and normal groups in TCGA-BLCA dataset via “DEseq2” R package (version 1.36.0) with |log2FC| > 1 and adj P < .05.[12]"}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed in the current study are available from the TCGA (https://portal.gdc.cancer.gov) and GEO (https://ww.ncbinlm.nih.gov/) databases [GSE13507].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39029056", "locator": "sections[27] \"fn-group\" paragraph 1", "quote": "The datasets used and/or analyzed in the current study are available from the TCGA (https://portal.gdc.cancer.gov) and GEO (https://ww.ncbinlm.nih.gov/) databases [GSE13507]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In total, 78 AR DEGs were acquired and a prognostic signature was build based on the 6 characteristic genes (CALR, FASN, CSPG4, HGF, INHBB, SATB1), where the patients of low-risk group had longer survival time.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39029056", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In total, 78 AR DEGs were acquired and a prognostic signature was build based on the 6 characteristic genes (CALR, FASN, CSPG4, HGF, INHBB, SATB1), where the patients of low-risk group had longer survival time."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "In total, 78 AR DEGs were acquired and a prognostic signature was build based on the 6 characteristic genes (CALR, FASN, CSPG4, HGF, INHBB, SATB1), where the patients of low-risk group had longer survival time.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39029056"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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