Differential miRNA and Protein Expression Reveals miR-1285, Its Targets TGM2 and CDH-1, as Well as CD166 and S100A13 as Potential New Biomarkers in Patients with Diabetes Mellitus and Pancreatic Adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39123454", "kind": "science-record", "revision": 4, "content_hash": "sha256:40868038158a6ae2b0b541bea9fcfb926c08ead5756f649bbccfc62ae2f8ef44", "created_at": "2026-11-01T20:29:13Z", "updated_at": "2026-11-01T22:54:54Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Differential miRNA and Protein Expression Reveals miR-1285, Its Targets TGM2 and CDH-1, as Well as CD166 and S100A13 as Potential New Biomarkers in Patients with Diabetes Mellitus and Pancreatic Adenocarcinoma.", "doi": "10.3390/cancers16152726", "pmid": "39123454", "authors": ["Theodoros Kolokotronis", "Britta Majchrzak-Stiller", "Marie Buchholz", "Vanessa Mense", "Johanna Strotmann", "Ilka Peters", "Lea Skrzypczyk", "Sven-Thorsten Liffers", "Louise Massia Menkene", "Mathias Wagner", "Matthias Glanemann", "Fay Betsou", "Wim Ammerlaan", "Ronny Schmidt", "Christoph Schröder", "Waldemar Uhl", "Chris Braumann", "Philipp Höhn"], "journal": "Cancers", "year": 2024, "abstract": "Early detection of PDAC remains challenging due to the lack of early symptoms and the absence of reliable biomarkers. The aim of the present project was to identify miRNA and proteomics signatures discriminating PDAC patients with DM from nondiabetic PDAC patients. Proteomics analysis and miRNA array were used for protein and miRNA screening. We used Western blotting and Real-Time Quantitative Reverse Transcription polymerase chain reaction (qRT-PCR) for protein and miRNA validation. Comparisons between experimental groups with normal distributions were performed using one-way ANOVA followed by Tukey's post hoc test, and pairwise tests were performed using t-tests. p ≤ 0.05 was considered statistically significant. Protein clusters of differentiation 166 (CD166), glycoprotein CD63 (CD63), S100 calcium-binding protein A13 (S100A13), and tumor necrosis factor-β (TNF-β) were detected in the proteomics screening. The miRNA assay revealed a differential miRNA 1285 regulation. Previously described target proteins of miR-1285 cadherin-1 (CDH-1), cellular Jun (c-Jun), p53, mothers against decapentaplegic homolog 4 (Smad4), human transglutaminase 2 (TGM2) and yes-associated protein (YAP), were validated via Western blotting. miR-1285-3p was successfully validated as differentially regulated in PDAC + DM via qRT-PCR. Overall, our data suggest miRNA1285-3p, TGM2, CDH-1, CD166, and S100A13 as potential meaningful biomarker candidates to characterize patients with PDAC + DM. Data are available via ProteomeXchange with the identifier PXD053169.", "claims": [{"id": "claim-method", "statement": "Comparisons between experimental groups with normal distributions were performed using one-way ANOVA followed by Tukey’s post hoc test, and pairwise tests were performed using unpaired t-tests. A linear model was fitted to the normalized data, and differential expression was assessed by computing the empirical Bayes statistics.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39123454", "locator": "sections[12] \"2.7. Statistics and Calculations\" paragraph 2", "quote": "Comparisons between experimental groups with normal distributions were performed using one-way ANOVA followed by Tukey’s post hoc test, and pairwise tests were performed using unpaired t-tests."}, {"asset": "source:pmid:39123454", "locator": "sections[8] \"2.3.3. miRNA Profiling Using a Takara Platform\" paragraph 2", "quote": "A linear model was fitted to the normalized data, and differential expression was assessed by computing the empirical Bayes statistics."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data that support the findings of this study have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD053169. Our data are available upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39123454", "locator": "sections[26] \"notes\" paragraph 1", "quote": "The mass spectrometry proteomics data that support the findings of this study have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD053169. 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