Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39182107", "kind": "science-record", "revision": 4, "content_hash": "sha256:fcd575c09be6d96a2775537631625441a5fd822b1240e9651e611e367f9a6092", "created_at": "2026-11-01T20:29:18Z", "updated_at": "2026-11-01T22:55:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor.", "doi": "10.1186/s12934-024-02510-1", "pmid": "39182107", "authors": ["Javier García-Martín", "Laura García-Abad", "Ramón I Santamaría", "Margarita Díaz"], "journal": "Microbial cell factories", "year": 2024, "abstract": "BACKGROUND: Several two-component systems of Streptomyces coelicolor, a model organism used for studying antibiotic production in Streptomyces, affect the expression of the bfr (SCO2113) gene that encodes a bacterioferritin, a protein involved in iron storage. In this work, we have studied the effect of the deletion mutant ∆bfr in S. coelicolor.\nRESULTS: The ∆bfr mutant exhibits a delay in morphological differentiation and produces a lesser amount of the two pigmented antibiotics (actinorhodin and undecylprodigiosin) compared to the wild type on complex media. The effect of iron in minimal medium was tested in the wild type and ∆bfr mutant. Consequently, we also observed different levels of production of the two pigmented antibiotics between the two strains, depending on the iron concentration and the medium (solid or liquid) used. Contrary to expectations, no differences in intracellular iron concentration were detected between the wild type and ∆bfr mutant. However, a higher level of reactive oxygen species in the ∆bfr mutant and a higher tolerance to oxidative stress were observed. Proteomic analysis showed no variation in iron response proteins, but there was a lower abundance of proteins related to actinorhodin and ribosomal proteins, as well as others related to secondary metabolite production and differentiation. Additionally, a higher abundance of proteins related to various types of stress, such as respiration and hypoxia among others, was also revealed. Data are available via ProteomeXchange with identifier PXD050869.\nCONCLUSION: This bacterioferritin in S. coelicolor (Bfr) is a new element in the complex regulation of secondary metabolism in S. coelicolor and, additionally, iron acts as a signal to modulate the biosynthesis of active molecules. Our model proposes an interaction between Bfr and iron-containing regulatory proteins. Thus, identifying these interactions would provide new information for improving antibiotic production in Streptomyces.", "claims": [{"id": "claim-method", "statement": "Two-way ANOVA (for experiments with two factors such as iron concentration and strain) with Holm-Šídák test for multiple comparisons (following software recommendations), and t-tests (for experiments with only two sets of measurements) were performed.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39182107", "locator": "sections[15] \"Statistical analysis\" paragraph 2", "quote": "Two-way ANOVA (for experiments with two factors such as iron concentration and strain) with Holm-Šídák test for multiple comparisons (following software recommendations), and t-tests (for experiments with only two sets of measurements) were performed."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium viathe PRIDE partner repository with the dataset identifier PXD050869 and 10.6019/PXD050869.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39182107", "locator": "sections[42] \"Data availability\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium viathe PRIDE partner repository with the dataset identifier PXD050869 and 10.6019/PXD050869."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39182107"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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