Proteomic analysis of cerebrospinal fluid of amyotrophic lateral sclerosis patients in the presence of autologous bone marrow derived mesenchymal stem cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39278909", "kind": "science-record", "revision": 3, "content_hash": "sha256:928024e77b6e83ef7ba9039d178b442f8de332b62c8b2f30d734ad07ad8f55a2", "created_at": "2026-11-01T20:29:26Z", "updated_at": "2026-11-01T22:12:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteomic analysis of cerebrospinal fluid of amyotrophic lateral sclerosis patients in the presence of autologous bone marrow derived mesenchymal stem cells.", "doi": "10.1186/s13287-024-03820-2", "pmid": "39278909", "authors": ["Ana Luiza Guimarães Reis", "Jessica Ruivo Maximino", "Luis Alberto de Padua Covas Lage", "Hélio Rodrigues Gomes", "Juliana Pereira", "Paulo Roberto Slud Brofman", "Alexandra Cristina Senegaglia", "Carmen Lúcia Kuniyoshi Rebelatto", "Debora Regina Daga", "Wellingson Silva Paiva", "Gerson Chadi"], "journal": "Stem cell research & therapy", "year": 2024, "abstract": "BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a fatal and rapidly progressive motoneuron degenerative disorder. There are still no drugs capable of slowing disease evolution or improving life quality of ALS patients. Thus, autologous stem cell therapy has emerged as an alternative treatment regime to be investigated in clinical ALS.\nMETHOD: Using Proteomics and Protein-Protein Interaction Network analyses combined with bioinformatics, the possible cellular mechanisms and molecular targets related to mesenchymal stem cells (MSCs, 1 × 106 cells/kg, intrathecally in the lumbar region of the spine) were investigated in cerebrospinal fluid (CSF) of ALS patients who received intrathecal infusions of autologous bone marrow-derived MSCs thirty days after cell therapy. Data are available via ProteomeXchange with identifier PXD053129.\nRESULTS: Proteomics revealed 220 deregulated proteins in CSF of ALS subjects treated with MSCs compared to CSF collected from the same patients prior to MSCs infusion. Bioinformatics enriched analyses highlighted events of Extracellular matrix and Cell adhesion molecules as well as related key targets APOA1, APOE, APP, C4A, C5, FGA, FGB, FGG and PLG in the CSF of cell treated ALS subjects.\nCONCLUSIONS: Extracellular matrix and cell adhesion molecules as well as their related highlighted components have emerged as key targets of autologous MSCs in CSF of ALS patients.\nTRIAL REGISTRATION: Clinicaltrial.gov identifier NCT0291768. Registered 28 September 2016.", "claims": [{"id": "claim-method", "statement": "Statistical analysis of LFQ data, employing the paired t-test and Benjamini-Hochberg correction, FDR < 0.05, p ≤ 0.05 (Graphpad Prism) identified deregulated proteins in CSF of ALS subjects, 30 days after MSCs infusion, compared to CSF of subjects before cell infusions [37, 38].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39278909", "locator": "sections[7] \"Statistical analysis\" paragraph 2", "quote": "Statistical analysis of LFQ data, employing the paired t-test and Benjamini-Hochberg correction, FDR < 0.05, p ≤ 0.05 (Graphpad Prism) identified deregulated proteins in CSF of ALS subjects, 30 days after MSCs infusion, compared to CSF of subjects before cell infusions [37, 38]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Raw data are available via ProteomeXchange with identifier PXD053129. Additional data used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39278909", "locator": "sections[31] \"notes\" paragraph 1", "quote": "Raw data are available via ProteomeXchange with identifier PXD053129. Additional data used and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Proteomics revealed 220 deregulated proteins in CSF of ALS subjects treated with MSCs compared to CSF collected from the same patients prior to MSCs infusion.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39278909", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Proteomics revealed 220 deregulated proteins in CSF of ALS subjects treated with MSCs compared to CSF collected from the same patients prior to MSCs infusion."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Proteomics revealed 220 deregulated proteins in CSF of ALS subjects treated with MSCs compared to CSF collected from the same patients prior to MSCs infusion.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39278909"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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