The prognostic value of bioinformatics analysis of ECM receptor signaling pathways and LAMB1 identification as a promising prognostic biomarker of lung adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39312319", "kind": "science-record", "revision": 3, "content_hash": "sha256:269922902a1b11b80eaf2fec12e1f503e0ac65c5c5e37ca203ebc6be5c16b016", "created_at": "2026-11-01T20:29:29Z", "updated_at": "2026-11-01T22:12:45Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The prognostic value of bioinformatics analysis of ECM receptor signaling pathways and LAMB1 identification as a promising prognostic biomarker of lung adenocarcinoma.", "doi": "10.1097/MD.0000000000039854", "pmid": "39312319", "authors": ["Tingjun Liu", "Jing Liu", "Quangang Chen", "Lianlian Wu", "Lingzhi Zhang", "Dandan Qiao", "Zhutao Huang", "Tianyuan Lu", "Ankang Hu", "Jie Wang"], "journal": "Medicine", "year": 2024, "abstract": "The extracellular matrix (ECM) is a complex and dynamic network of cross-linked proteins and a fundamental building block in multicellular organisms. Our study investigates the impact of genes related to the ECM receptor interaction pathway on immune-targeted therapy and lung adenocarcinoma (LUAD) prognosis. This study obtained LUAD chip data (GSE68465, GSE31210, and GSE116959) from NCBI GEO. Moreover, the gene data associated with the ECM receptor interaction pathway was downloaded from the Molecular Signature Database. Differentially expressed genes were identified using GEO2R, followed by analyzing their correlation with immune cell infiltration. Univariate Cox regression analysis screened out ECM-related genes significantly related to the survival prognosis of LUAD patients. Additionally, Lasso regression and multivariate Cox regression analysis helped construct a prognostic model. Patients were stratified by risk score and survival analyses. The prognostic models were evaluated using receiver operating characteristic curves, and risk scores and prognosis associations were analyzed using univariate and multivariate Cox regression analyses. A core gene was selected for gene set enrichment analysis and CIBERSORT analysis to determine its function and tumor-infiltrating immune cell proportion, respectively. The results revealed that the most abundant pathways among differentially expressed genes in LUAD primarily involved the cell cycle, ECM receptor interaction, protein digestion and absorption, p53 signaling pathway, complement and coagulation cascade, and tyrosine metabolism. Two ECM-associated subtypes were identified by consensus clustering. Besides, an ECM-related prognostic model was validated to predict LUAD survival, and it was associated with the tumor immune microenvironment. Additional cross-analysis screened laminin subunit beta 1 (LAMB1) for further research. The survival time of LUAD patients with elevated LAMB1 expression was longer than those with low LAMB1 expression. Gene set enrichment analysis and CIBERSORT analyses revealed that LAMB1 expression correlated with tumor immune microenvironment. In conclusion, a prognostic model of LUAD patients depending on the ECM receptor interaction pathway was constructed. Screening out LAMB1 can become a prognostic risk factor for LUAD patients or a potential target during LUAD treatment.", "claims": [{"id": "claim-method", "statement": "Using the Lasso–Cox regression model for 10-fold cross-validation, we obtained ECM-related genes significantly correlated with overall survival (OS), and a prognostic model was developed.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39312319", "locator": "sections[9] \"2.7. Construction of prognostic model and evaluation of prognostic performance\" paragraph 2", "quote": "Using the Lasso–Cox regression model for 10-fold cross-validation, we obtained ECM-related genes significantly correlated with overall survival (OS), and a prognostic model was developed."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All the data used in this study was open-access data from online databases, so ethical approval was not necessary in our research.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39312319", "locator": "sections[24] \"fn-group\" paragraph 1", "quote": "All the data used in this study was open-access data from online databases, so ethical approval was not necessary in our research."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The areas under the Receiver Operating Curve for overall survival at 1, 3, and 5 years were 0.67, 0.69, and 0.74, respectively (Fig. 5E).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39312319", "locator": "sections[16] \"3.4. Construction and evaluation of a prognostic model of ECM receptor interaction pathway\" paragraph 2", "quote": "The areas under the Receiver Operating Curve for overall survival at 1, 3, and 5 years were 0.67, 0.69, and 0.74, respectively (Fig. 5E)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The areas under the Receiver Operating Curve for overall survival at 1, 3, and 5 years were 0.67, 0.69, and 0.74, respectively (Fig. 5E).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39312319"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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