Generational Diet-Induced Obesity Remodels the Omental Adipose Proteome in Female Mice.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39339686", "kind": "science-record", "revision": 2, "content_hash": "sha256:cace60d58f8bda5572d7e61e4076160c1edb995f21631099ed5cf54aef713d01", "created_at": "2026-11-01T20:29:32Z", "updated_at": "2026-11-01T21:34:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Generational Diet-Induced Obesity Remodels the Omental Adipose Proteome in Female Mice.", "doi": "10.3390/nu16183086", "pmid": "39339686", "authors": ["Naviya Schuster-Little", "Morgan McCabe", "Kayla Nenninger", "Reihaneh Safavi-Sohi", "Rebecca J Whelan", "Tyvette S Hilliard"], "journal": "Nutrients", "year": 2024, "abstract": "Obesity, a complex condition that involves genetic, environmental, and behavioral factors, is a non-infectious pandemic that affects over 650 million adults worldwide with a rapidly growing prevalence. A major contributor is the consumption of high-fat diets, an increasingly common feature of modern diets. Maternal obesity results in an increased risk of offspring developing obesity and related health problems; however, the impact of maternal diet on the adipose tissue composition of offspring has not been evaluated. Here, we designed a generational diet-induced obesity study in female C57BL/6 mice that included maternal cohorts and their female offspring fed either a control diet (10% fat) or a high-fat diet (45% fat) and examined the visceral adipose proteome. Solubilizing proteins from adipose tissue is challenging due to the need for high concentrations of detergents; however, the use of a detergent-compatible sample preparation strategy based on suspension trapping (S-Trap) enabled label-free quantitative bottom-up analysis of the adipose proteome. We identified differentially expressed proteins related to lipid metabolism, inflammatory disease, immune response, and cancer, providing valuable molecular-level insight into how maternal obesity impacts the health of offspring. Data are available via ProteomeXchange with the identifier PXD042092.", "claims": [{"id": "claim-method", "statement": "The log2 fold change was calculated for each comparison group, and p-values were calculated using Student’s t-test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39339686", "locator": "sections[8] \"2.6. Data Analysis\" paragraph 3", "quote": "The log2 fold change was calculated for each comparison group, and p-values were calculated using Student’s t-test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics datasets generated for this study can be found in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD042092.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39339686", "locator": "sections[21] \"notes\" paragraph 1", "quote": "The mass spectrometry proteomics datasets generated for this study can be found in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD042092."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The generational obesity comparison group contained the greatest number of differentially regulated proteins out of all seven group comparisons, with 23 up-regulated and 43 down-regulated proteins in the generational obese cohort (Wes(WES)) compared to the control-only fed mice (Con(CON)).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39339686", "locator": "sections[12] \"3.3. Protein Expression Changes in Murine Omenta Are Diet-Dependent\" paragraph 2", "quote": "The generational obesity comparison group contained the greatest number of differentially regulated proteins out of all seven group comparisons, with 23 up-regulated and 43 down-regulated proteins in the generational obese cohort (Wes(WES)) compared to the control-only fed mice (Con(CON)) (Figure 3C)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The generational obesity comparison group contained the greatest number of differentially regulated proteins out of all seven group comparisons, with 23 up-regulated and 43 down-regulated proteins in the generational obese cohort (Wes(WES)) compared to the control-only fed mice (Con(CON)).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39339686"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Mass spectrometry-grade trypsin gold was ordered from Promega", "ed in technical duplicate for a total of six LC-MS/MS analyses per dietary cohort. Statistical ana", "e dissected [20]. 2.4. Protein Isolation and Proteomics Sample Processing Proteins were isolated fro"], "order_basis": "paper_text_order:2059", "id": "assay:1"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["imental Design and Statistical Rationale All murine studies were approved by the Institutional A", "out using Graph Pad Prism 9.0 software. 2.3. Murine Generational Obesity Animal Model The pre-cl", "tional Obesity Animal Model The pre-clinical murine model of diet-induced obesity (DIO) includes", "y = Wes(CON); Generational Obese = Wes(WES). 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