Integrin subunit beta 6 is a potential diagnostic marker for acute kidney injury in patients with diabetic kidney disease: a single cell sequencing data analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39356055", "kind": "science-record", "revision": 4, "content_hash": "sha256:314726ec121aa7994c0e127873fbc08179823e0c368d469cff9a068004f2e6ac", "created_at": "2026-11-01T20:29:34Z", "updated_at": "2026-11-01T22:55:19Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrin subunit beta 6 is a potential diagnostic marker for acute kidney injury in patients with diabetic kidney disease: a single cell sequencing data analysis.", "doi": "10.1080/0886022X.2024.2409348", "pmid": "39356055", "authors": ["Congcong Yao", "Ziwei Li", "Hongshuang Su", "Keke Sun", "Qihui Liu", "Yan Zhang", "Lishuang Zhu", "Feng Jiang", "Yaguang Fan", "Songtao Shou", "Heng Wu", "Heng Jin"], "journal": "Renal failure", "year": 2024, "abstract": "BACKGROUND: Diabetic kidney disease (DKD), a prevalent complication of diabetes mellitus, is often associated with acute kidney injury (AKI). Thus, the development of preventive and therapeutic strategies is crucial for delaying the progression of AKI and DKD.\nMETHODS: The GSE183276 dataset, comprising the data of 20 healthy controls and 12 patients with AKI, was downloaded from the Gene Expression Omnibus (GEO) database to analyze the AKI group. For analyzing the DKD group, the GSE131822 dataset, comprising the data of 3 healthy controls and 3 patients with DKD, was downloaded from the GEO database. The common differentially expressed genes (DEGs) in renal tubular epithelial cells (TECs) were subjected to enrichment analyses. Next, a protein-protein interaction (PPI) network was constructed using the Search Tool for the Retrieval of Interacting Genes database to analyze gene-related regulatory networks. Finally, the AKI animal models and the DKD and AKI cell models were established, and the reliability of the identified genes was validated using quantitative real-time polymerase chain reaction analysis.\nRESULTS: Functional analysis was performed with 40 common DEGs in TECs. Eight hub genes were identified using the PPI and gene-related networks. Finally, validation experiments with the in vivo animal model and the in vitro cellular model revealed the four common DEGs. Four DEGs that share molecular mechanisms in the pathogenesis of DKD and AKI were identified. In particular, the expression of Integrin Subunit Beta 6(ITGB6), a hub and commonly upregulated gene, was upregulated in the in vitro models.\nCONCLUSION: ITGB6 may serve as a biomarker for early AKI diagnosis in patients with DKD and as a target for early intervention therapies.", "claims": [{"id": "claim-method", "statement": "Additionally, the means between the groups were compared using one-way analysis of variance.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39356055", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Additionally, the means between the groups were compared using one-way analysis of variance."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All materials are owned by the authors and/or no permissions are required. If you need more details, please contact Heng Jin (E-mail: hengjin@tmu.edu.cn).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39356055", "locator": "sections[27] \"Data availability statement\" paragraph 2", "quote": "All materials are owned by the authors and/or no permissions are required. If you need more details, please contact Heng Jin (E-mail: hengjin@tmu.edu.cn)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Four DEGs that share molecular mechanisms in the pathogenesis of DKD and AKI were identified.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39356055", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Four DEGs that share molecular mechanisms in the pathogenesis of DKD and AKI were identified."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Four DEGs that share molecular mechanisms in the pathogenesis of DKD and AKI were identified.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39356055"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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