Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Psoriasis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39376789", "kind": "science-record", "revision": 4, "content_hash": "sha256:349386d18a4c0db16a54aeb5fc071802ce585bf463ac1aa9b40f16acaf624ba3", "created_at": "2026-11-01T20:29:35Z", "updated_at": "2026-11-01T22:55:21Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Psoriasis.", "doi": "10.2147/CCID.S484034", "pmid": "39376789", "authors": ["Cailing E", "Rongying Wang", "Zudong Meng", "Yulin Zou"], "journal": "Clinical, cosmetic and investigational dermatology", "year": 2024, "abstract": "Background: Psoriasis, a chronic and recurrent inflammatory skin disease, current treatments can only alleviate its symptoms. There is still no complete cure. Although increasing research supports the therapeutics to be better, the common mechanism of its occurrence is still not fully elucidated. Our study is about further explore the molecular mechanism of the occurrence of this disease.\nMethods: The gene expression profiles of psoriasis (GSE151177, GSE41664, GSE30999) were downloaded from the Gene Expression Omnibus (GEO) database. After identifying the common differentially expressed genes (DEGs) of psoriasis using R software, three kinds of analyses were performed, namely WGCNA, GWAS Analysis, Drug Target Prediction.\nResults: A total of 14 common DEGs was selected for subsequent analyses. Our Drug Target Prediction analysis revealed that the expression profiles influenced by certain drugs, including methotrexate, budesonide, amino purvalanol-a, and selumetinib, exhibited negative correlations with the disease-perturbed expression profiles. Finally, It was found that S100A4, JAML, TRAF3IP3, MIAT, IL7R, and KLRB1 were prominently expressed in the immune pathway related to allograft rejection. In the metabolic pathway, oxidative phosphorylation showed high expression levels, while the reactive oxygen species pathway was notably expressed in the signaling pathways domain.\nConclusion: Our study reveals the potential drugs and pathogenesis of psoriasis. These potential pathway and hub genes may provide new ideas for further mechanism research.", "claims": [{"id": "claim-method", "statement": "Leveraging the limma package, this investigation quantitatively assessed the differential gene expression within the GSE30999 dataset to pinpoint genes variably expressed in psoriasis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39376789", "locator": "sections[25] \"Drug Target Prediction Using Cmap\" paragraph 2", "quote": "Leveraging the limma package, this investigation quantitatively assessed the differential gene expression within the GSE30999 dataset to pinpoint genes variably expressed in psoriasis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39376789", "locator": "sections[30] \"Data Sharing Statement\" paragraphs 1-4", "quote": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Analysis of GEO expression data identified six key psoriasis genes—S100A4, JAML, TRAF3IP3, MIAT, IL7R, and KLRB1—candidate drugs, and associated immune and metabolic pathways.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39376789", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Our study reveals the potential drugs and pathogenesis of psoriasis. These potential pathway and hub genes may provide new ideas for further mechanism research."}, {"asset": "source:pmid:39376789", "locator": "sections[20] \"Feature Selection via Lasso Regression and SVM\" paragraph 3", "quote": "We selected these six genes S100A4, JAML, TRAF3IP3, MIAT, IL7R, and KLRB1 as key genes for subsequent research."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Analysis of GEO expression data identified six key psoriasis genes—S100A4, JAML, TRAF3IP3, MIAT, IL7R, and KLRB1—candidate drugs, and associated immune and metabolic pathways.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39376789"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE151177"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE151177"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:39376789", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/CCID.S484034", "sha256": "sha256:fc120794288566a727be6fdb88bbfe14b6ccb87c616da32722f50659f08de0c0", "bytes": 41785, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.2147/CCID.S484034", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC11457778", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE151177", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE151177", "version": null, "note": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177].", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM4567877", "GSM4567878", "GSM4567879", "GSM4567880", "GSM4567881", "GSM4567882", "GSM4567883", "GSM4567884", "GSM4567885", "GSM4567886", "GSM4567887", "GSM4567888", "GSM4567889", "GSM4567890", "GSM4567891", "GSM4567892", "GSM4567893", "GSM4567894", "GSM4567895", "GSM4567896", "GSM4567897", "GSM4567898", "GSM4567899"], "sample_count": 23, "group_sizes": null, "group_sizes_basis": null, "platform": "18573", "taxon": "Homo sapiens", "title": "Single-cell RNA sequencing of emigrating cells from human psoriasis skin and control normal skin", "resolution_checked_at": "2026-11-01T20:16:08Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE30999", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE30999", "version": null, "note": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177].", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM767976", "GSM767977", "GSM767978", "GSM767979", "GSM767980", "GSM767981", "GSM767982", "GSM767983", "GSM767984", "GSM767985", "GSM767986", "GSM767987", "GSM767988", "GSM767989", "GSM767990", "GSM767991", "GSM767992", "GSM767993", "GSM767994", "GSM767995", "GSM767996", "GSM767997", "GSM767998", "GSM767999", "GSM768000", "GSM768001", "GSM768002", "GSM768003", "GSM768004", "GSM768005", "GSM768006", "GSM768007", "GSM768008", "GSM768009", "GSM768010", "GSM768011", "GSM768012", "GSM768013", "GSM768014", "GSM768015", "GSM768016", "GSM768017", "GSM768018", "GSM768019", "GSM768020", "GSM768021", "GSM768022", "GSM768023", "GSM768024", "GSM768025", "GSM768026", "GSM768027", "GSM768028", "GSM768029", "GSM768030", "GSM768031", "GSM768032", "GSM768033", "GSM768034", "GSM768035", "GSM768036", "GSM768037", "GSM768038", "GSM768039", "GSM768040", "GSM768041", "GSM768042", "GSM768043", "GSM768044", "GSM768045", "GSM768046", "GSM768047", "GSM768048", "GSM768049", "GSM768050", "GSM768051", "GSM768052", "GSM768053", "GSM768054", "GSM768055", "GSM768056", "GSM768057", "GSM768058", "GSM768059", "GSM768060", "GSM768061", "GSM768062", "GSM768063", "GSM768064", "GSM768065", "GSM768066", "GSM768067", "GSM768068", "GSM768069", "GSM768070", "GSM768071", "GSM768072", "GSM768073", "GSM768074", "GSM768075", "GSM768076", "GSM768077", "GSM768078", "GSM768079", "GSM768080", "GSM768081", "GSM768082", "GSM768083", "GSM768084", "GSM768085", "GSM768086", "GSM768087", "GSM768088", "GSM768089", "GSM768090", "GSM768091", "GSM768092", "GSM768093", "GSM768094", "GSM768095", "GSM768096", "GSM768097", "GSM768098", "GSM768099", "GSM768100", "GSM768101", "GSM768102", "GSM768103", "GSM768104", "GSM768105", "GSM768106", "GSM768107", "GSM768108", "GSM768109", "GSM768110", "GSM768111", "GSM768112", "GSM768113", "GSM768114", "GSM768115", "GSM768116", "GSM768117", "GSM768118", "GSM768119", "GSM768120", "GSM768121", "GSM768122", "GSM768123", "GSM768124", "GSM768125", "GSM768126", "GSM768127", "GSM768128", "GSM768129", "GSM768130", "GSM768131", "GSM768132", "GSM768133", "GSM768134", "GSM768135", "GSM768136", "GSM768137", "GSM768138", "GSM768139", "GSM768140", "GSM768141", "GSM768142", "GSM768143", "GSM768144", "GSM768145"], "sample_count": 170, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Expression data from skin biopsy samples from patients with moderate-to-severe psoriasis", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE41664", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE41664", "version": null, "note": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177].", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM1021248", "GSM1021249", "GSM1021250", "GSM1021251", "GSM1021252", "GSM1021253", "GSM1021254", "GSM1021255", "GSM1021256", "GSM1021257", "GSM1021258", "GSM1021259", "GSM1021260", "GSM1021261", "GSM1021262", "GSM1021263", "GSM1021264", "GSM1021265", "GSM1021266", "GSM1021267", "GSM1021268", "GSM1021269", "GSM1021270", "GSM1021271", "GSM1021272", "GSM1021273", "GSM1021274", "GSM1021275", "GSM1021276", "GSM1021277", "GSM1021278", "GSM1021279", "GSM1021280", "GSM1021281", "GSM1021282", "GSM1021283", "GSM1021284", "GSM1021285", "GSM1021286", "GSM1021287", "GSM1021288", "GSM1021289", "GSM1021290", "GSM1021291", "GSM1021292", "GSM1021293", "GSM1021294", "GSM1021295", "GSM1021296", "GSM1021297", "GSM1021298", "GSM1021299", "GSM1021300", "GSM1021301", "GSM1021302", "GSM1021303", "GSM1021304", "GSM1021305", "GSM1021306", "GSM1021307", "GSM1021308", "GSM1021309", "GSM1021310", "GSM1021311", "GSM1021312", "GSM1021313", "GSM1021314", "GSM1021315", "GSM1021316", "GSM1021317", "GSM1021318", "GSM1021319", "GSM1021320", "GSM1021321", "GSM1021322", "GSM1021323", "GSM1021324", "GSM1021325", "GSM1021326", "GSM1021327", "GSM1021328", "GSM1021329", "GSM1021330", "GSM1021331", "GSM1021332", "GSM1021333", "GSM1021334", "GSM1021335", "GSM1021336", "GSM1021337", "GSM1021338", "GSM1021339", "GSM1021340", "GSM1021341", "GSM1021342", "GSM1021343", "GSM1021344", "GSM1021345", "GSM1021346", "GSM1021347", "GSM1021348", "GSM1021349", "GSM1021350", "GSM1021351", "GSM1021352", "GSM1021353", "GSM1021354", "GSM1021355", "GSM1021356", "GSM1021357", "GSM1021358", "GSM1021359", "GSM1021360", "GSM1021361", "GSM1021362", "GSM1021363", "GSM1021364", "GSM1021365", "GSM1021366", "GSM1021367", "GSM1021368", "GSM1021369", "GSM1021370", "GSM1021371", "GSM1021372", "GSM1021373", "GSM1021374", "GSM1021375", "GSM1021376", "GSM845740", "GSM845741", "GSM845742", "GSM845743", "GSM845744", "GSM845745", "GSM845746", "GSM845747", "GSM845748", "GSM845749", "GSM845750", "GSM845751", "GSM845752", "GSM845753", "GSM845754", "GSM845755", "GSM845756", "GSM845757", "GSM845758", "GSM845759", "GSM845760", "GSM845761", "GSM845762", "GSM845763", "GSM845764", "GSM845765", "GSM845766", "GSM845767"], "sample_count": 157, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Comparison of Gene Expression in Psoriatic Skin from Different Sources", "resolution_checked_at": "2026-11-01T20:16:08Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE151177 not downloaded", "asset:data:geo:GSE151177 licence not read", "asset:data:geo:GSE30999 not downloaded", "asset:data:geo:GSE30999 licence not read", "asset:data:geo:GSE41664 not downloaded", "asset:data:geo:GSE41664 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "differential_expression", "specific": "limma_voom", "label": "Differential expression analysis", "dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["count_matrix_with_condition"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Leveraging the limma package, this investigation quantitatively assessed the differential gene expression within the GSE30999 dataset to pinpoint genes variably expressed in psoriasis."}}, {"id": "method-2", "family": "genetic_association", "specific": "gwas", "label": "Genetic association (GWAS / linkage / MR)", "dsl_operations": ["gwas_locus_scan"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["genotype_phenotype_table"], "evidence": {"score": 0.7, "band": "medium", "quote": "GWAS"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: limma_voom."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE151177", "GSE41664", "GSE30999"], "resolved_accessions": ["GSE151177", "GSE41664", "GSE30999"], "statements": ["The datasets generated and analysed during the current study are available in the GEO database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30999, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41664].\nThe datasets generated and analysed during the current study are available in the scRNA database repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151177]."], "basis": "The paper names GSE151177, GSE41664, GSE30999 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "assets": ["source:pmid:39376789", "data:geo:GSE151177"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE151177"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T19:34:24Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 5, "usage": {"prompt_tokens": 10506, "completion_tokens": 1415, "cached_tokens": 264}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:39376789", "type": "paper", "label": "pmid:39376789"}, {"id": "source:pmid:39376789", "type": "manuscript", "label": "PMID 39376789 full text"}, {"id": "data:geo:GSE151177", "type": "dataset", "label": "GEO GSE151177"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:39376789", "type": "paper", "label": "pmid:39376789"}, {"id": "source:pmid:39376789", "type": "manuscript", "label": "PMID 39376789 full text"}, {"id": "data:geo:GSE151177", "type": "dataset", "label": "GEO GSE151177"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-39376789", "type": "extraction", "used": ["source:pmid:39376789"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE151177", "type": "accession_resolution", "used": ["data:geo:GSE151177"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:39376789", "parent_hash": "sha256:87aeac53dcba67269d1511b2490d92af1488483c16735263c7109621dc53b1f6", "fork_of": null, "generation": 3}, "gaps": ["asset:data:geo:GSE151177 licence not read", "asset:data:geo:GSE151177 not downloaded", "asset:data:geo:GSE30999 licence not read", "asset:data:geo:GSE30999 not downloaded", "asset:data:geo:GSE41664 licence not read", "asset:data:geo:GSE41664 not downloaded", "execution.not_run", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Leveraging the limma package, this investigation quantitatively assessed the differential gene expression within the GSE30999 dataset to pinpoint genes variably expressed in psoriasis."], "order_basis": "analysis_only", "id": "assay:1"}]}