SLAMF8 regulates osteogenesis and adipogenesis of bone marrow mesenchymal stem cells via S100A6/Wnt/β-catenin signaling pathway.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39380096", "kind": "science-record", "revision": 4, "content_hash": "sha256:4fb8c2a8458d1c1b2a9a071efc5e43cbeb39a5df65962a7ca12d7aaddc81587a", "created_at": "2026-11-01T20:29:36Z", "updated_at": "2026-11-01T22:55:21Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "SLAMF8 regulates osteogenesis and adipogenesis of bone marrow mesenchymal stem cells via S100A6/Wnt/β-catenin signaling pathway.", "doi": "10.1186/s13287-024-03964-1", "pmid": "39380096", "authors": ["Yibo Wang", "Kai Hang", "Xiaoyong Wu", "Li Ying", "Zhongxiang Wang", "Zemin Ling", "Hao Hu", "Zhijun Pan", "Xuenong Zou"], "journal": "Stem cell research & therapy", "year": 2024, "abstract": "BACKGROUND: The inflammatory microenvironment plays an essential role in bone healing after fracture. The signaling lymphocytic activation molecule family (SLAMF) members deeply participate in inflammatory response and make a vast difference.\nMETHODS: We identified SLAMF8 in GEO datasets (GSE129165 and GSE176086) and co-expression analyses were performed to define the relationships between SLAMF8 and osteogenesis relative genes (RUNX2 and COL1A1). In vitro, we established SLAMF8 knockdown and overexpression mouse bone marrow mesenchymal stem cells (mBMSCs) lines. qPCR, Western blot, ALP staining, ARS staining, Oil Red O staining and Immunofluorescence analyses were performed to investigate the effect of SLAMF8 in mBMSCs osteogenesis and adipogenesis. In vivo, mice femoral fracture model was performed to explore the function of SLAMF8.\nRESULTS: SLAMF8 knockdown significantly suppressed the expression of osteogenesis relative genes (RUNX2, SP7 and COL1A1), ALP activity and mineral deposition, but increased the expression of adipogenesis relative genes (PPARγ and C/EBPα). Additionally, SLAMF8 overexpression had the opposite effects. The role SLAMF8 played in mBMSCs osteogenic and adipogenic differentiation were through S100A6 and Wnt/β-Catenin signaling pathway. Moreover, SLAMF8 overexpression mBMSCs promoted the healing of femoral fracture.\nCONCLUSIONS: SLAMF8 promotes osteogenesis and inhibits adipogenesis of mBMSCs via S100A6 and Wnt/β-Catenin signaling pathway. SLAMF8 overexpression mBMSCs effectively accelerate the healing of femoral fracture in mice.", "claims": [{"id": "claim-method", "statement": "Student t test was used to compared the data between 2 groups and One-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test was used to compared the data involves multiple group comparisons.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39380096", "locator": "sections[14] \"Statistical analysis\" paragraph 2", "quote": "Student t test was used to compared the data between 2 groups and One-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test was used to compared the data involves multiple group comparisons."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The gene expression databases of BMSCs treated with TNFα (50ng/mL or 100ng/mL) were downloaded from Gene Expression Omnibus (GEO) datasets (GSE129165 and GSE176086). The raw expression data of SLAMF8, OSA1, RSAD2, COL1A1, RUNX2, PPARG and CEBPA genes were downloaded from the Cancer Cell Line Encyclopedia (CCLE) project (https://portals.broadinstitute.org/ccle), The Cancer Genome Atlas (TCGA) project (http://cancergenome.nih.gov/) and the Genotype Tissue Expression (GTEx) project (https://www.gtexportal.org/). The mass spectrometry data was archived in Supplementary Data 1.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39380096", "locator": "sections[28] \"Data availability\" paragraph 2", "quote": "The gene expression databases of BMSCs treated with TNFα (50ng/mL or 100ng/mL) were downloaded from Gene Expression Omnibus (GEO) datasets (GSE129165 and GSE176086). The raw expression data of SLAMF8, OSA1, RSAD2, COL1A1, RUNX2, PPARG and CEBPA genes were downloaded from the Cancer Cell Line Encyclopedia (CCLE) project (https://portals.broadinstitute.org/ccle), The Cancer Genome Atlas (TCGA) project (http://cancergenome.nih.gov/) and the Genotype Tissue Expression (GTEx) project (https://www.gtexportal.org/). The mass spectrometry data was archived in Supplementary Data 1."}], "state": "complete"}, {"id": "claim-target-result", "statement": "SLAMF8 promotes osteogenesis and inhibits adipogenesis of mBMSCs via S100A6 and Wnt/β-Catenin signaling pathway.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39380096", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "SLAMF8 promotes osteogenesis and inhibits adipogenesis of mBMSCs via S100A6 and Wnt/β-Catenin signaling pathway."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "SLAMF8 promotes osteogenesis and inhibits adipogenesis of mBMSCs via S100A6 and Wnt/β-Catenin signaling pathway.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39380096"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE129165"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:student_t", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE129165"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:student_t"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:39380096", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s13287-024-03964-1", "sha256": "sha256:4a42d5716b72d8691a88fc125fd225f72b1ef53cb0486a175cf155cacd51e8cd", "bytes": 67529, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s13287-024-03964-1", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC11462740", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE129165", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE129165", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE129165", "version": null, "note": "The gene expression databases of BMSCs treated with TNFα (50ng/mL or 100ng/mL) were downloaded from Gene Expression Omnibus (GEO) datasets (GSE129165 and GSE176086). 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In vitro, we established SLAMF8 knockdown and overexpression mouse bone marrow mesench", "lore the function of SLAMF8. RESULTS: SLAMF8 knockdown significantly suppressed the expression of o", "e stable SLAMF8 overexpression cell line was transfected by lentivirus (Tsingke Biotechnology, Beijin", "n of SLAMF8 and S100A6 in mBMSCs. Cells were transfected with SLAMF8-siRNA, S100A6-siRNA and negative"], "order_basis": "paper_text_order:568", "id": "assay:1"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["MF8 in mBMSCs osteogenesis and adipogenesis. In vivo, mice femoral fracture model was performed t", "y accelerate the healing of femoral fracture in mice.", "ablished SLAMF8 knockdown and overexpression mouse bone marrow mesenchymal stem cells (mBMSCs)", "ods Cell culture and differentiation Primary mouse bone marrow stem cells (mBMSCs) were purchas"], "order_basis": "paper_text_order:597", "id": "assay:2"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["arrow mesenchymal stem cells (mBMSCs) lines. qPCR, Western blot, ALP staining, ARS staining, O"], "order_basis": "paper_text_order:654", "id": "assay:3"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["mesenchymal stem cells (mBMSCs) lines. qPCR, Western blot, ALP staining, ARS staining, Oil Red O stain"], "order_basis": "paper_text_order:660", "id": "assay:4"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["aining, ARS staining, Oil Red O staining and Immunofluorescence analyses were performed to investigate the e"], "order_basis": "paper_text_order:725", "id": "assay:5"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["(MUBMX-01001, Guangzhou, China). mBMSCs were cultured in α-MEM medium with 10% FBS (Cyagen, FBSAD-010", "lybrene. After 48 h, cells were passaged and cultured with 2 µg/mL Puromycin Dihydrochloride (ST551, Be", "nd 4 µg/mL polybrene. After 48 h, cells were passaged and cultured with 2 µg/mL Puromycin Dihydroc"], "order_basis": "paper_text_order:1835", "id": "assay:6"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Student t test was used to compared the data between 2 groups and One-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test was used to compared the data involves multiple group comparisons."], "order_basis": "consumes_earlier_output", "id": "assay:7"}]}