Prognostic biomarkers based on GUF1, EFTUD2 and GSPT1 targets affecting migration of gastric cancer cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39430826", "kind": "science-record", "revision": 2, "content_hash": "sha256:f36f136f2b5ec852233a5a0803bbb90d1c6f4b871d5ca05b3e7b2b665f5da465", "created_at": "2026-11-01T20:29:41Z", "updated_at": "2026-11-01T21:34:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Prognostic biomarkers based on GUF1, EFTUD2 and GSPT1 targets affecting migration of gastric cancer cells.", "doi": "10.21037/tcr-24-125", "pmid": "39430826", "authors": ["Haixiu Ma", "Lina Suo", "Jing Zhao", "Ronghua Ma", "Qi Wang", "Jun Liu", "Jinwan Qiao", "Juan Wu", "Juan An", "Yan Liu", "Yonghua Xing", "Haiyan Wang", "Zhanhai Su"], "journal": "Translational cancer research", "year": 2024, "abstract": "Background: Eukaryotic elongation factor 1 alpha 2 (eEF1A2) is a protein coding gene which is involved in tumor development and progression in several types of human cancer, but little is known about the function of eEF1A2 proteins in gastric cancer (GC). This study aimed to investigate the effects of GUF1, EFTUD2 and GSPT1 on the migration of GC cells.\nMethods: The Oncomine and The Cancer Genome Atlas (TCGA) databases were used to evaluate the expression of GUF1, EFTUD2, GSPT1 and GSPT2 in GC and the association of eEF1A2 family with individual clinical characteristics. Kaplan-Meier (K-M) Plotter hinted the prognostic value of GUF1, EFTUD2, GSPT1 and GSPT2. GSE62254 and GSE66222 datasets were used to validate the expression of GUF1, EFTUD2, GSPT1. AGS cell line and GES line were also used for validating the function of GUF1, EFTUD2, GSPT1. RNA interference (RNAi) of GUF1, EFTUD2 and GSPT1 had been used to query those genes expression pattern and dissect the proliferation and migration in GC cell lines.\nResults: GUF1, EFTUD2 and GSPT1 were significantly up-regulated in GC cell lines. High expression of GUF1, EFTUD2 and GSPT1 was correlated with cell proliferation and migration induced in GC cells. GUF1, EFTUD2 and GSPT1 may be potential novel oncogenes that helps to maintain the survival of GC cells.\nConclusions: This study identified that high levels of GUF1, EFTUD2 and GSPT1 expression are predictive biomarkers for a poor prognosis in GC.", "claims": [{"id": "claim-method", "statement": "Receiver operating characteristic (ROC) curves for each gene are shown in Figure 5.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39430826", "locator": "sections[22] \"Diagnostic capability of GUF1, EFTUD2 and GSPT1 for GC\" paragraph 2", "quote": "Receiver operating characteristic (ROC) curves for each gene are shown in Figure 5."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Available at https://tcr.amegroups.com/article/view/10.21037/tcr-24-125/dss\n10.21037/tcr-24-125", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39430826", "locator": "sections[33] \"Data Sharing Statement\" paragraphs 1-3", "quote": "Available at https://tcr.amegroups.com/article/view/10.21037/tcr-24-125/dss\n10.21037/tcr-24-125"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39430826"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE62254"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE62254"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:39430826", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.21037/tcr-24-125", "sha256": 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