Comparative Analysis of Plasma Protein Dynamics in Women with ST-Elevation Myocardial Infarction and Takotsubo Syndrome.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39513871", "kind": "science-record", "revision": 2, "content_hash": "sha256:0f0a9b01c1dca210eecd82aeb02c81cbc32177265faf59b7bdbe71e9bb527fe9", "created_at": "2026-11-01T20:29:46Z", "updated_at": "2026-11-01T21:34:17Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Comparative Analysis of Plasma Protein Dynamics in Women with ST-Elevation Myocardial Infarction and Takotsubo Syndrome.", "doi": "10.3390/cells13211764", "pmid": "39513871", "authors": ["Shafaat Hussain", "Sandeep Jha", "Evelin Berger", "Linnea Molander", "Valentyna Sevastianova", "Zahra Sheybani", "Aaron Shekka Espinosa", "Ahmed Elmahdy", "Amin Al-Awar", "Yalda Kakaei", "Mana Kalani", "Ermir Zulfaj", "Amirali Nejat", "Abhishek Jha", "Tetiana Pylova", "Maryna Krasnikova", "Erik Axel Andersson", "Elmir Omerovic", "Björn Redfors"], "journal": "Cells", "year": 2024, "abstract": "BACKGROUND: ST-elevation myocardial infarction (STEMI) and Takotsubo syndrome (TS) are two distinct cardiac conditions that both result in sudden loss of cardiac dysfunction and that are difficult to distinguish clinically. This study compared plasma protein changes in 24 women with STEMI and 12 women with TS in the acute phase (days 0-3 post symptom onset) and the stabilization phase (days 7, 14, and 30) to examine the molecular differences between these conditions.\nMETHODS: Plasma proteins from STEMI and TS patients were extracted during the acute and stabilization phases and analyzed via quantitative proteomics. Differential expression and functional significance were assessed. Data are accessible on ProteomeXchange, ID PXD051367.\nRESULTS: During the acute phase, STEMI patients showed higher levels of myocardial inflammation and tissue damage proteins compared to TS patients, along with reduced tissue repair and anti-inflammatory proteins. In the stabilization phase, STEMI patients exhibited ongoing inflammation and disrupted lipid metabolism. Notably, ADIPOQ was consistently downregulated in STEMI patients in both phases. When comparing the acute to the stabilization phase, STEMI patients showed increased inflammatory proteins and decreased structural proteins. Conversely, TS patients showed increased proteins involved in inflammation and the regulatory response to counter excessive inflammation. Consistent protein changes between the acute and stabilization phases in both conditions, such as SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE, suggest shared underlying pathophysiological mechanisms.\nCONCLUSIONS: This study presents protein changes in women with STEMI or TS and identifies ADIPOQ, SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE as candidates for further exploration in both therapeutic and diagnostic contexts.", "claims": [{"id": "claim-method", "statement": "Differential expression analysis was conducted in R version 4.3.2 using a two-sample t-test and paired t-test on log-transformed data to identify proteins with significant differences. To control multiple testing, the Benjamini–Hochberg procedure was employed.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39513871", "locator": "sections[10] \"2.5.3. Statistical Analysis\" paragraph 2", "quote": "Differential expression analysis was conducted in R version 4.3.2 using a two-sample t-test and paired t-test on log-transformed data to identify proteins with significant differences. To control multiple testing, the Benjamini–Hochberg procedure was employed."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry and Spectronaut analysis files have been deposited with the ProteomeXchange consortium via the MassIVE repository (dataset identifier MSV000094514) and are accessible on PRIDE via ID PXD051367 [53].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39513871", "locator": "sections[32] \"Data Availability Statement\" paragraph 2", "quote": "The mass spectrometry and Spectronaut analysis files have been deposited with the ProteomeXchange consortium via the MassIVE repository (dataset identifier MSV000094514) and are accessible on PRIDE via ID PXD051367 [53]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Notably, ADIPOQ was consistently downregulated in STEMI patients in both phases.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39513871", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Notably, ADIPOQ was consistently downregulated in STEMI patients in both phases."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Notably, ADIPOQ was consistently downregulated in STEMI patients in both phases.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39513871"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_unresolved", "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD051367"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD051367"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:obtain_source_data blocked on data:accession_unresolved"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:39513871", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3390/cells13211764", "sha256": "sha256:959afa098914cf974c1e0a15a4f35d69ec335d83c819559c1fdb92d29c3724e6", "bytes": 67023, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3390/cells13211764", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC11545104", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD051367", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD051367", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD051367", "version": null, "note": "The mass spectrometry and Spectronaut analysis files have been deposited with the ProteomeXchange consortium via the MassIVE repository (dataset identifier MSV000094514) and are accessible on PRIDE via ID PXD051367 [53].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The mass spectrometry and Spectronaut analysis files have been deposited with the ProteomeXchange consortium via the MassIVE repository (dataset identifier MSV000094514) and are accessible on PRIDE via ID PXD051367 [53].", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": null, "resolution_checked_at": "2026-11-01T20:16:17Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:PXD051367 not downloaded", "asset:data:geo:PXD051367 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "principal_component_analysis", "specific": "pca", "label": "Principal component analysis", "dsl_operations": ["pca_fit"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["wide_numeric_matrix"], "evidence": {"score": 0.8, "band": "high", "quote": "Principal component analysis"}}, {"id": "method-2", "family": null, "specific": null, "label": "Two-sample t-test and paired t-test on log-transformed data, with Benjamini–Hochberg correction", "dsl_operations": [], "missing_operations": [], "expressible": null, "parameters": {}, "data_shapes": [], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Differential expression analysis was conducted in R version 4.3.2 using a two-sample t-test and paired t-test on log-transformed data to identify proteins with significant differences. 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