MiR-155-5p regulates autophagy and apoptosis of glioma cells through RICTOR.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39525027", "kind": "science-record", "revision": 4, "content_hash": "sha256:cd43774af3894d0feed9d4da8d5f6ecc21c72da3aca6cb44480aff27bf332d32", "created_at": "2026-11-01T20:29:47Z", "updated_at": "2026-11-01T22:55:35Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "MiR-155-5p regulates autophagy and apoptosis of glioma cells through RICTOR.", "doi": "10.21037/tcr-24-543", "pmid": "39525027", "authors": ["Zhao Guo", "Jing-Jie Tian", "Yao Wang", "Lei Jiang", "Yang Chen", "He-Jun Dai", "Lei Wang", "Yi Zhang"], "journal": "Translational cancer research", "year": 2024, "abstract": "Background: Glioma characterized by the high degree of drug resistance and the poor prognosis is the most common primary malignant tumors of the brain. And miRNA is involved in a variety of biological behaviors of tumors, enhancing or inhibiting the occurrence and development of tumors. Therefore, the present study aims to explore whether miR-155-5p can regulate autophagy and apoptosis of glioma through RICTOR.\nMethods: The significantly differential gene miR-155-5p was identified from the Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo) databases GSE165937 and GSE138764 using bioinformatics analysis, and its expression was validated by quantitative real-time polymerase chain reaction (qRT-PCR). The putative target genes of miR-155-5p were predicted through interrogation of relevant databases, followed by identification of key target genes. Subsequently, core target genes were selected for functional enrichment analysis. The U87MG cell line was utilized as the experimental model and divided into Negative Control1 (NC1) group, Mimic group, Negative Control2 (NC2) group, Inhibitor group, and NC + 3-methyladenine (3-MA) group. The expression levels of miR-155-5p, RICTOR, P62, LC-3, Bax, Bcl-2, and Caspase-3 were assessed using qRT-PCR, cellular fluorescence imaging, and Western blotting; while apoptosis in the U87MG cell line was evaluated via flow cytometry.\nResults: The results showed that miR-155-5P was highly expressed in glioma cells, which could inhibit the expression of Bax, Caspase-3, LCII/LCI and Beclin-1, and increase the expression of Bcl2 and P62. Flow cytometry and cell fluorescence were used to verify the above results. Moreover, when U87MG cells treated with miR-155-5p inhibitor were inhibited by 3-MA, the results showed that miR-155-5p enhanced the anti-apoptotic ability of U87MG cells by regulating autophagy. In addition, the bioinformatics results show that miR-155-5p survival prognosis in glioma into a strong negative correlation, while the survival prognosis of RICTOR in glioma showed a strong positive correlation. The core target genes Kyoto Encyclopedia of Genes and Genomes (KEGG) mainly occurred in PI3K-AKT signaling pathway; in addition, qRT-PCR and Western blot confirmed the regulatory effect of miR-155-5P on RICTOR.\nConclusions: MiR-155-5p regulates autophagy and apoptosis-related proteins in glioma cells through RICTOR, affecting the occurrence and development of glioma.", "claims": [{"id": "claim-method", "statement": "The t-test was used for pairwise comparison, and one-way analysis of variance (ANOVA) was used for comparison among multiple groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39525027", "locator": "sections[10] \"Statistical analysis\" paragraph 2", "quote": "The t-test was used for pairwise comparison, and one-way analysis of variance (ANOVA) was used for comparison among multiple groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Available at https://tcr.amegroups.com/article/view/10.21037/tcr-24-543/dss", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39525027", "locator": "sections[23] \"Data Sharing Statement\" paragraph 2", "quote": "Available at https://tcr.amegroups.com/article/view/10.21037/tcr-24-543/dss"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39525027"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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Methods: The signi", "escence imaging, and Western blotting; while apoptosis in the U87MG cell line was evaluated via flo", "clusions: MiR-155-5p regulates autophagy and apoptosis-related proteins in glioma cells through RIC"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["qRT-PCR, cellular fluorescence imaging, and Western blotting; while apoptosis in the U87MG cell line was", "signaling pathway; in addition, qRT-PCR and Western blot confirmed the regulatory effect of miR-155-5"], "order_basis": "paper_text_order:1335", "id": "assay:2"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["sis in the U87MG cell line was evaluated via flow cytometry. 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