Investigation of CST7 and hsa-miR-4793-5p gene expression in breast cancer.

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In this research, our objective was to examine the CST7 and has-miR-4793-5p gene expression in BC tumor tissues and adjacent normal tissues. Using GSE57897 gene expression data from 422 BC samples and 31 breast samples from healthy controls which was based on the Platform GPL18722 (spotted oligonucleotide Homo sapiens microRNA (miRNA) array) in the Gene Expression Omnibus (GEO) and compare with miRNAs with a conserved target location on CST7 mRNA were found using databases. The study population included 60 fresh BC tissue samples and adjacent normal tissues as control. The Quantitative Real-Time PCR was used to evaluate the expression levels of CST7 and has-miR-4793-5p in the breast tissues. The present study, found that CST7 and hsa-miR-4793-5p were significantly increased in tumoral tissues in compare to normal tissues. Further analysis revealed a remarkable association between CST7 and hsa-miR-4793-5p gene expression alteration. ROC curve analysis demonstrated high accuracy for CST7 expression in BC tumors. Comparison of gene expression between different stages and patient family history showed significant findings. Due to the high sensitivity and specificity of the expression changes of these two genes, they are suitable candidates for further investigations to be considered as part of a diagnosis and prognosis panel.", "claims": [{"id": "claim-method", "statement": "The t-test was employed to ascertain the rate of change in expression data, ROC curve analysis was used to evaluate sensitivity and specificity and AUC was used for reporting.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39552709", "locator": "sections[7] \"Statistical analysis\" paragraph 2", "quote": "The t-test was employed to ascertain the rate of change in expression data, ROC curve analysis was used to evaluate sensitivity and specificity and AUC was used for reporting."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data will be made available on request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39552709", "locator": "sections[20] \"Data availability\" paragraph 2", "quote": "Data will be made available on request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "CST7 and hsa-miR-4793-5p expression demonstrated 2.99 (P < 0.0001) and 3.91-fold (P < 0.0001) increase in tumoral tissues respectively (Fig. 3A and B).", "kind": "quantitative", "target": {"value": 3.91, "unit": "fold change", "tolerance": null, "source": "CST7 and hsa-miR-4793-5p expression demonstrated 2.99 (P < 0.0001) and 3.91-fold (P < 0.0001) increase in tumoral tissues respectively (Fig. 3A and B)."}, "evidence": [{"asset": "source:pmid:39552709", "locator": "sections[10] \"CST7 and hsa-miR-4793-5p gene expression\" paragraph 2", "quote": "CST7 and hsa-miR-4793-5p expression demonstrated 2.99 (P < 0.0001) and 3.91-fold (P < 0.0001) increase in tumoral tissues respectively (Fig. 3A and B)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "CST7 and hsa-miR-4793-5p expression demonstrated 2.99 (P < 0.0001) and 3.91-fold (P < 0.0001) increase in tumoral tissues respectively (Fig. 3A and B).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39552709"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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