hsa-miR-181a-5p inhibits glioblastoma development via the MAPK pathway: in-silico and in-vitro study.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39574474", "kind": "science-record", "revision": 4, "content_hash": "sha256:bb70bda759835db099e82307c721c745550368f21b81bcad839d6e8b9a8feb15", "created_at": "2026-11-01T20:29:50Z", "updated_at": "2026-11-01T22:55:39Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "hsa-miR-181a-5p inhibits glioblastoma development via the MAPK pathway: in-silico and in-vitro study.", "doi": "10.32604/or.2024.051569", "pmid": "39574474", "authors": ["Mahdi Abdoli Shadbad", "Behzad Baradaran"], "journal": "Oncology research", "year": 2024, "abstract": "Background: Glioblastoma remains a highly invasive primary brain malignancy with an undesirable prognosis. Growing evidence has shed light on the importance of microRNAs (miRs), as small non-coding RNAs, in tumor development and progression. The present study leverages the in-silico and in-vitro techniques to investigate the significance of hsa-miR-181a-5p and the underlying hsa-miR-181a-5p-meidated signaling pathway in glioblastoma development.\nMethods: Bioinformatic studies were performed on GSE158284, GSE108474 (REMBRANDT study), TCGA-GTEx, CCLE, GeneMANIA, Reactome, WikiPathways, KEGG, miRDB, and microT-CDS to identify the significance of hsa-miR-181a-5p and its underlying target. Afterward, the U373 cell line was selected and transfected with hsa-miR-181a-5p mimics, and the cell viability, clonogenicity, migration, mRNA expression, apoptosis, and cell cycle were studied using the MTT assay, colony formation test, migration assay, qRT-PCR, and flow cytometry respectively.\nResults: hsa-miR-181a-5p expression is decreased in glioblastoma samples. The in-silico results have shown that hsa-miR-181a-5p could regulate the MAPK pathway by targeting AKT3. The experimental assays have shown that hsa-miR-181a-5p decreases the migration of glioblastoma cells, arrests the cell cycle, and increases the apoptosis rate. Besides downregulating MMP9 and upregulating BAX, hsa-miR-181a-5p downregulates MET, MAP2K1, MAPK1, MAPK3, and AKT3 expression in U373 cells. The in-vitro results were consistent with in-silico results regarding the regulatory effect of hsa-miR-181a-5p on the MAPK pathway, leading to tumor suppression in glioblastoma.\nConclusions: hsa-miR-181a-5p inhibits glioblastoma development partially by regulating the signaling factors of the MAPK pathway.", "claims": [{"id": "claim-method", "statement": "The unpaired student t-test was employed for statistical analysis comparing two groups, whereas the one-way ANOVA was utilized for comparing 2 < groups. The Shapiro–Wilk test was utilized to study the normality of the data, and the F test was used to compare the variance of data in the unpaired student t-tests. The p-values below 0.05 were statistically significant.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39574474", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "The unpaired student t-test was employed for statistical analysis comparing two groups, whereas the one-way ANOVA was utilized for comparing 2 < groups. The Shapiro–Wilk test was utilized to study the normality of the data, and the F test was used to compare the variance of data in the unpaired student t-tests. The p-values below 0.05 were statistically significant."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets analyzed during the current study are available in the GSE158284 (https://www.ncbi.nlm.nih.gov/geo/), GSE108474 (https://www.ncbi.nlm.nih.gov/geo/), TCGA-GTEx (http://gepia.cancer-pku.cn/), and CCLE (https://sites.broadinstitute.org/ccle/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39574474", "locator": "sections[27] \"Availability of Data and Materials\" paragraph 2", "quote": "The datasets analyzed during the current study are available in the GSE158284 (https://www.ncbi.nlm.nih.gov/geo/), GSE108474 (https://www.ncbi.nlm.nih.gov/geo/), TCGA-GTEx (http://gepia.cancer-pku.cn/), and CCLE (https://sites.broadinstitute.org/ccle/)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "hsa-miR-181a-5p inhibits glioblastoma development partially by regulating the signaling factors of the MAPK pathway.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39574474", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "hsa-miR-181a-5p inhibits glioblastoma development partially by regulating the signaling factors of the MAPK pathway."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "hsa-miR-181a-5p inhibits glioblastoma development partially by regulating the signaling factors of the MAPK pathway.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39574474"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE108474"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:student_t", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE108474"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:student_t"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:39574474", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.32604/or.2024.051569", "sha256": "sha256:e69655595e62e8fdc83da2ec11e56234946c2c9fd8f83835d0da783b409df87f", "bytes": 37138, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.32604/or.2024.051569", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC11576920", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE108474", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE108474", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE108474", "version": null, "note": "The datasets analyzed during the current study are available in the GSE158284 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