FCGBP functions as a tumor suppressor gene in head and neck squamous cell carcinoma.

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It was urgent to uncover novel molecules that play vital role in HNSCC for disease monitoring and drug development.\nMETHODS: Distinguished expression of FCGBP mRNA in HNSCC was analyzed by TCGA-HNSC and three GEO datasets, the relationship between FCGBP and clinical stage and survival was analyzed by GEPIA 2, the immune infiltration pattern analysis was conducted by TIMER 2.0, pathways affected by FCGBP was conducted by GSEA and GO/KEGG. In vitro experiments (including qRT-PCR, siRNA transfection, CCK8, transwell assay and flow cytometry) were conducted to confirm bioinformatic analysis.\nRESULTS: FCGBP was down-regulated in tumor samples compared with normal tissues at both mRNA and protein levels, and positively correlated with survival in HNSCC. Genes co-expressed with FCGBP were mainly enriched in immune-related biological processes and pathways. GSEA indicated that FCGBP was associated with activated immune reaction and inhibiting well-known pro-tumor pathways. GSE41613 validated FCGBP as an independent prognostic marker for HNSCC and FCGBP was down-regulated in HNSCC cell lines by qRT-PCR. Migration and invasion of SCC9 and CAL27 were enhanced by FCGBP-targeting siRNAs, the ratio of cytotoxic T lymphocytes were down-regulated while the ratio of myeloid-derived suppressor cells were increased by FCGBP-targeting siRNAs.\nCONCLUSION: FCGBP was a tumor suppressor gene and was an independent prognostic marker for better survival. The underlying mechanism may be that FCGBP inhibited tumor migration and invasion and activated immune response against tumor cells.", "claims": [{"id": "claim-method", "statement": "By analyzing GSE41613 dataset, FCGBP was an independent prognostic marker for HNSCC and negatively correlated with patients survival, which was analyzed by uni-variate cox, multivariate cox and Kaplan–Meier methods (Fig. 4a–c).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39580769", "locator": "sections[22] \"FCGBP was an independent prognostic marker for HNSCC by analyzing GEO dataset and validation of its mRNA level by in vitro experiments\" paragraph 2", "quote": "By analyzing GSE41613 dataset, FCGBP was an independent prognostic marker for HNSCC and negatively correlated with patients survival, which was analyzed by uni-variate cox, multivariate cox and Kaplan–Meier methods (Fig. 4a–c)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39580769", "locator": "sections[31] \"Data availability\" paragraph 2", "quote": "The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "FCGBP was a tumor suppressor gene and was an independent prognostic marker for better survival.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39580769", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "FCGBP was a tumor suppressor gene and was an independent prognostic marker for better survival."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "FCGBP was a tumor suppressor gene and was an independent prognostic marker for better survival.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39580769"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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