Deep Crotonylproteomics Analysis of High-Altitude Exposure Hearts in Mice.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39619542", "kind": "science-record", "revision": 2, "content_hash": "sha256:0c2f0a257593f5a0c3924202804a5f0f9f2a8a92ed87ff2a395e24dd59f29c69", "created_at": "2026-11-01T20:29:53Z", "updated_at": "2026-11-01T21:34:25Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Deep Crotonylproteomics Analysis of High-Altitude Exposure Hearts in Mice.", "doi": "10.1021/acsomega.4c05818", "pmid": "39619542", "authors": ["Tian Yue", "Jiali Yang", "Jian He", "ShiQiang Xiong", "Han Gao", "Shanshan Qin", "Dunzhu Danzeng", "Junbo Xu", "Jun Hou"], "journal": "ACS omega", "year": 2024, "abstract": "The potential molecular mechanisms of short-term adaptation and long-term structural deteriorations in the cardiac structure in response to high altitude remain not fully understood. This study aims to investigate changes in the crotonylproteome in the hearts of mice exposed to high altitude at different time points. The hearts were obtained from mice living at the lowland and 3, 10, and 30 days after arriving at a plateau. Crotonyllysolylated proteins were subsequently comprehensively evaluated. Among the identified 13,731 crotonyllysine sites, 11,140 were quantified. Furthermore, 1247 differentially expressed crotonyllysine sites on 764 proteins in 46 pathways were analyzed, and the results showed that the abundance of crotonylation increased with the increase of exposure time to altitude. Functional enrichment analysis showed that most of these significantly altered crotonylated proteins were enriched in the regulation of metabolism and myocardial function, indicating that these enriched biological processes may be involved in the acute adaptation and chronic remodeling of cardiac function and structural changes at a high altitude. Our study uncovers some crotonylation-affected processes and pathways in the heart's response to high-altitude exposure, giving novel insights into molecular processes of cardiac function and structure changes at high altitude (ProteomeXchange identifier: PXD044178).", "claims": [{"id": "claim-method", "statement": "After this round of screening, the remaining 1247 lysine sites were used for expression pattern clustering analysis using the Mfuzz method.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39619542", "locator": "sections[10] \"Mfuzz Cluster Analysis\" paragraph 2", "quote": "After this round of screening, the remaining 1247 lysine sites were used for expression pattern clustering analysis using the Mfuzz method."}], "state": "complete"}, {"id": "claim-data-access", "statement": "MS raw files are deposited in a public repository PRIDE/ProteomeXchange under the identifier PXD044178.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39619542", "locator": "sections[20] \"notes\" paragraph 1", "quote": "MS raw files are deposited in a public repository PRIDE/ProteomeXchange under the identifier PXD044178."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Furthermore, 1247 differentially expressed crotonyllysine sites on 764 proteins in 46 pathways were analyzed, and the results showed that the abundance of crotonylation increased with the increase of exposure time to altitude.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39619542", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Furthermore, 1247 differentially expressed crotonyllysine sites on 764 proteins in 46 pathways were analyzed, and the results showed that the abundance of crotonylation increased with the increase of exposure time to altitude."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Furthermore, 1247 differentially expressed crotonyllysine sites on 764 proteins in 46 pathways were analyzed, and the results showed that the abundance of crotonylation increased with the increase of exposure time to altitude.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39619542"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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