Development and validation of a prognostic and drug sensitivity model for gastric cancer utilizing telomere-related genes.

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"section_index": 13, "section_title": "Gene expression analysis of TCGA-STAD cohort", "row_count": 27, "column_count": 5}, "table": {"label": "Table 1", "caption": "Patient baseline characteristics.", "rows": [["Characters", "TCGAN = 334", "Risk-HighN = 167", "Risk-LowN = 167", "P-value"], ["Age", "", "", "", "0.045"], ["<60", "104", "61", "43", ""], ["≥60", "227", "106", "121", ""], ["Unknown", "3", "0", "3", ""], ["Gender", "", "", "", "0.210"], ["Female", "120", "66", "54", ""], ["Male", "214", "101", "113", ""], ["Stage", "", "", "", "0.279"], ["I", "46", "17", "29", ""], ["II", "110", "57", "53", ""], ["III", "144", "74", "70", ""], ["IV", "34", "19", "15", ""], ["T", "", "", "", "0.278"], ["T1", "15", "4", "11", ""], ["T2", "70", "36", "34", ""], ["T3", "156", "77", "79", ""], ["T4", "93", "50", "43", ""], ["N", "", "", "", "0.033"], ["N0", "103", "42", "61", ""], ["N+", "230", "124", "106", ""], ["Unknown", "1", "1", "0", ""], ["G", "", "", "", "0.015"], ["G1", "8", "2", "6", 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"MiniMax-M3.1-Flash", "prompt_version": "full-text-reading-v1", "prompt_hash": "e8bf2f669409682961d95892f37999c4b15fe56a3526f7dd54badbc41bdcecbe", "source_hash": "80e36cb31f11e813b441e1a7e891322e7d9d789ff22ba149348b8fec2ade6b8f", "raw_response_sha256": "sha256:d8eb40865d219d8a67015abd845f575eba3e532e7873408f5b49a77b60b53abb", "raw_response_chars": 5671}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "full-text-reading-v1", "prompt_hash": "f6b5975428b437ac2f7c806216cad4344fa8d4df177298f5d41a659ef704bbaa", "source_hash": "80e36cb31f11e813b441e1a7e891322e7d9d789ff22ba149348b8fec2ade6b8f", "raw_response_sha256": "sha256:7f4d24af34945559745e8e65bed674a4655e773e54e7dd6d1d42d4211ee1ffe2", "raw_response_chars": 68749}], "target_providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "replication-target-v2", "prompt_hash": "1f12eccd71ad0710d19a33c43ab8b292ce023f5e541eb8755f77168f53253f0e", "source_hash": "80e36cb31f11e813b441e1a7e891322e7d9d789ff22ba149348b8fec2ade6b8f", "raw_response": "{\"parent_value\":4,\"unit\":\"genes\",\"evidence\":\"A predictive risk model composed of four telomere-related genes (TRGs) was established, enabling the accurate stratification of GC patients into two distinct prognostic groups.\",\"target_result\":\"The predictive risk model was composed of four telomere-related genes.\",\"number_stated\":true}", "reading": {"parent_value": 4, "unit": "genes", "evidence": "A predictive risk model composed of four telomere-related genes (TRGs) was established, enabling the accurate stratification of GC patients into two distinct prognostic groups.", "target_result": "The predictive risk model was composed of four telomere-related genes.", "number_stated": true, "source_locator": "chars 1159-1335 of source sha256 80e36cb31f11e813b441e1a7e891322e7d9d789ff22ba149348b8fec2ade6b8f"}, "recorded_at": "2026-10-31T15:19:21.687489+00:00"}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "replication-target-v2", "prompt_hash": "a8b93891b41e064261b363d92cc2115b8560477559b1dbb1ca856aa1a94c3cae", "source_hash": "80e36cb31f11e813b441e1a7e891322e7d9d789ff22ba149348b8fec2ade6b8f", "raw_response": "<think>We need answer JSON only. Need identify single numerical result replication of registered target headline numerical result paper states main finding. Need interpret. Source has many numbers. Registered target = headline numerical result paper states for main finding. Main finding prognostic telomere gene model stratified GC into two distinct prognostic groups. Headline number likely HR 1.89 for high risk score? Need understand expected extraction task. User asks \"find the single numerical result that a replication of the registered target has to reproduce. Report the headline number only: the value the paper itself states for the registered target, not a value you calculated, converted, averaged or reconstructed. The unit must be the unit the paper prints. The evidence must be an exact, contiguous quotation from SOURCE TEXT that contains the number ... Attribution may be carried by text's own wording: a number may be attached to the target by a parenthetical list, by the word 'respectively', or by words that name the target directly. When that is how the text links them, quote the whole clause so a reader can check the attribution for themselves. If text states no number for registered target...\" We need decide target_result and numerical value.\n\n\"REGISTERED TARGET: the headline numerical result the paper states for its main finding\" Perhaps target is broad main finding, and headline numerical result likely \"328\" DEGs? Or \"two\" prognostic groups? Or four genes? Need parse source. Title and abstract-ish: model can stratified into two distinct prognostic groups; high-risk immune-cold; PARP inhibitors. Results: 328 DEGs, 35 associated prognosis, model composed of four genes, stratified into two groups. Which is \"headline numerical result\" paper states for main finding? Replication target perhaps number of genes in model? They ask \"single numerical result\" and unit printed. Could be \"4 genes\". Evidence exact quote: \"A predictive risk model composed of four telomere-related genes (TRGs) was established, enabling the accurate stratification of GC patients into two distinct prognostic groups.\" This contains 4 and 2, ambiguity. Headline number maybe risk score HR 1.89.\n\nNeed infer benchmark framing. User likely asks to extract a specific statistic for \"registered target\" from source, with target potentially phrase abstract. They explicitly say \"the headline numerical result the paper itself states for its main finding\". We need select likely headline numerical result. Could be the abstract result typically includes sample size, AUC, HR etc. But supplied source excerpt perhaps article partial and no abstract. Main finding has high risk score HR 1.89. A replication of prognostic model main finding must reproduce high-risk hazard ratio 1.89? \"unit must be unit paper prints\" For HR unit could be \"HR\" perhaps. Parent_value number. Evidence exact clause. target_result explain. Yet maybe headline is \"197 drugs\" in drug sensitivity main result? Main paper's headline finding perhaps model with four TRGs, two groups. Numerical result to reproduce likely \"four telomere-related genes\". Replication of target registered as main finding would need reproduce model gene count = 4. Unit \"genes\". Evidence entire clause. Could be.\n\nLet's inspect wording. They ask \"Read the supplied paper text and find the single numerical result that a replication of the registered target has to reproduce.\" Registered target maybe generated from paper metadata? \"the headline numerical result the paper itself states for its main finding\". Main finding could be abstract conclusion. We need identify \"single numerical result\". Often these tasks ask answer a question where target is result, often \"What was [main result]?\" They demand one number. Here registered target is vague intentionally. Need select most headline-like metric.\n\nSource text includes detailed results, likely target perhaps main finding's \"prognostic risk model\". Main numerical finding: high risk score independent variable for OS HR 1.89 (95% CI 1.33–2.68, P &lt; 0.001). If replicate main finding, reproduce HR 1.89. The word \"headline numerical result\" points to effect size, not counts. Also \"unit must be the unit the paper prints\": HR has dimensionless ratio, perhaps \"HR\" as unit? Could set unit \"HR\". They want headline number only, so 1.89, evidence whole Cox clause. target_result \"The paper reports a hazard ratio of 1.89 for a high risk score as an independent predictor of overall survival.\" Unit maybe \"hazard ratio (HR)\". The source prints \"HR 1.89\", so unit \"HR\" or perhaps no unit. User says unit paper prints, likely \"HR\". parent_value 1.89.\n\nNeed be certain. Could be number of distinct prognostic groups = two. Main finding exactly says \"can stratified gastric cancer patients into two distinct prognostic groups.\" That's likely headline numerical result. A replication of main finding must reproduce two groups. Unit \"prognostic groups\". Evidence exact quote from abstract/highlights. The \"headline numerical result the paper itself states for its main finding\" perhaps indeed \"two distinct prognostic groups\" is the most prominent headline. The source begins title then highlights:\n\"The telomere-related gene model can stratified gastric cancer patients into two distinct prognostic groups.\nThe high-risk group displayed an “immune-cold” subtype with increased regulatory T cell infiltration.\nPatients in the high-risk group may benefit from treatment with PARP inhibitors.\"\nMain finding headline = stratification into two distinct prognostic groups. Numerical result = two. \"Unit\" perhaps \"groups\". The paper prints \"two distinct prognostic groups.\" Then exact quote. But a replication of registered target (\"headline numerical result the paper itself states for its main finding\") might target this.\n\nQuestion perhaps designed to test extracting \"headline number\" in paper, avoiding numeric details. Registered target could correspond to \"how many distinct prognostic groups can model stratify patients into?\" Answer 2. They phrase target not as direct question but \"the headline numerical result the paper itself states for its main finding.\" The strongest headline in source is two distinct prognostic groups. They might expect parent_value 2, unit \"distinct prognostic groups\", evidence exact sentence.\n\nLet's parse every numeric statement and relevance:\n- 328 telomere-related DEGs\n- 35 associated prognosis\n- model four TRGs\n- two prognostic groups\n- 334 patients\n- 8939 DEGs, 5713 upregulated, 2093 TRGs, 328 overlaps\n- age HR etc\n- 197 drugs different IC50\n- drug names\n- thresholds.\nMain finding likely model composition and stratification. \"headline numerical result\" singular perhaps count of genes = four. The source's Results first paragraph:\n\"Differential expression analysis identified 328 significantly telomere-related DEGs in GC, with 35 of them showing a significant association with GC prognosis. A predictive risk model composed of four telomere-related genes (TRGs) was established, enabling the accurate stratification of GC patients into two distinct prognostic groups.\"\nHeadline numerical result of model = four genes. But registered target says \"the headline numerical result the paper itself states for its main finding,\" not \"number of genes\". Which numerical result is main? Could be 4 TRGs, because paper title says model utilizing telomere-related genes and conclusion \"four TRGs\". Yet the high-level highlighted main finding says \"two distinct prognostic groups.\" \n\nMaybe \"replication of the registered target\" language comes from a benchmark where target is a paper's result claim, and asks numerical result to reproduce. They might expect one \"parent_value\" like sample size, AUC, etc. Need use clues in schema field \"parent_value\". Maybe they ask \"parent_value\" because could have multiple values? This schema often used in \"scientific claim numeric value\" extraction. Target_result string. They often want numerical result tied to target, exact quote. The registered target here is not specific enough, so perhaps intended answer based on headline sentence near top.\n\n\"The telomere-related gene model can stratified gastric cancer patients into two distinct prognostic groups.\" This is unequivocally the first bullet, directly states headline finding. Number is written as word \"two\". Unit \"distinct prognostic groups\". Evidence quote whole sentence. target_result perhaps \"The model stratifies gastric cancer patients into two distinct prognostic groups.\" number_stated true, parent_value 2.\n\nBut phrase \"headline numerical result the paper itself states for its main finding\" likely points at that. If target were high risk score HR, they'd register \"hazard ratio for high risk score\" rather than vague headline. The registered target is intentionally semantic, and first sentence is obvious.\n\nCould also be \"four telomere-related genes\" because headline main finding in title/model. But perhaps they expect \"2\" from prominent synopsis. Let's analyze wording \"single numerical result that a replication of the registered target has to reproduce.\" Replication of \"the headline numerical result the paper itself states for its main finding\". A paper's main finding might be \"telomere-related gene model stratifies GC patients into two distinct prognostic groups.\" To reproduce that finding, one must reproduce group count 2. Yes.\n\nNeed output exact JSON no markdown. Evidence exact contiguous quote containing number. If number is written \"two\", quote:\n\"The telomere-related gene model can stratified gastric cancer patients into two distinct prognostic groups.\"\nThis contains number. Unit must unit paper prints: perhaps \"distinct prognostic groups\". Set unit \"prognostic groups\" maybe \"distinct prognostic groups\". parent_value 2.\n\ntarget_result should state what text states: \"The telomere-related gene model stratifies gastric cancer patients into two distinct prognostic groups.\" Preserve grammatical typo? Could paraphrase in target_result; instruction evidence no paraphrase only. target_result could mirror source: \"The telomere-related gene model can stratified gastric cancer patients into two distinct prognostic groups.\" Better exact wording perhaps. number_stated true.\n\nBut let's not rush. Need assess if \"headline numerical result\" might refer to \"328\" because it appears in Results first and abstract-like summary. Maybe replication of main finding needs reproduce 328 DEGs? No, main finding is model, not DEG discovery. \"two groups\" is central.\n\nCould unit be \"prognostic groups\" or \"patients\"? The printed phrase \"two distinct prognostic groups\" means unit/count noun \"distinct prognostic groups\". We can use \"distinct prognostic groups\". parent_value number 2.\n\nEvidence exact quote, no ellipsis. Include punctuation. JSON escapes quotes? No internal quotes. Fine.\n\nYet user says \"Report the headline number only: the value ... The unit must be the unit the paper prints.\" Could evidence necessarily includes sentence. They want only one numerical value in parent_value and unit. We comply. target_result can include two perhaps. They say headline number only maybe don't report CI or P. If HR route, we'd report only 1.89, but evidence clause would contain other numbers—which exact quote clause has CI and P, violating perhaps \"headline number only\" means output parent value only; evidence can contain all. They explicitly say quote whole clause when attribution parenthetical etc. Here high risk HR clause contains several numbers, potentially confusing. This perhaps argues intended target is a clean sentence \"two distinct prognostic groups.\" Also source highlight sentence exactly clean.\n\nLet's formulate JSON:\n{\"parent_value\":2,\"unit\":\"distinct prognostic groups\",\"evidence\":\"The telomere-related gene model can stratified gastric cancer patients into two distinct prognostic groups.\",\"target_result\":\"The telomere-related gene model can stratify gastric cancer patients into two distinct prognostic groups.\",\"number_stated\":true}\n\nNeed exact source typo: \"can stratified\". Evidence exact. Target_result can improve grammar but perhaps avoid adding. Use exact source perhaps.\n\nWould \"unit\" singular? \"prognostic groups\" as printed. User says unit paper prints; exact unit phrase perhaps \"distinct prognostic groups\". Fine.\n\nLet's ensure valid JSON and no extra.</think>\n\n{\"parent_value\":2,\"unit\":\"distinct prognostic groups\",\"evidence\":\"The telomere-related gene model can stratified gastric cancer patients into two distinct prognostic groups.\",\"target_result\":\"The telomere-related gene model stratifies gastric cancer patients into two distinct prognostic groups.\",\"number_stated\":true}", "reading": {"parent_value": 2, "unit": "distinct prognostic groups", "evidence": "The telomere-related gene model can stratified gastric cancer patients into two distinct prognostic groups.", "target_result": "The telomere-related gene model stratifies gastric cancer patients into two distinct prognostic groups.", "number_stated": true, "source_locator": "chars 122-229 of source sha256 80e36cb31f11e813b441e1a7e891322e7d9d789ff22ba149348b8fec2ade6b8f"}, "recorded_at": "2026-10-31T15:19:36.883431+00:00"}], "agreement": false, "disagreements": {"target_result": {"minimax": "A predictive risk model composed of four telomere-related genes (LRRN1, SNCG, GAMT, and PDE1B) was established, stratifying gastric cancer patients into high- and low-risk prognostic groups.", "minimax_alt": "The headline finding was a four-gene prognostic risk model comprising LRRN1, SNCG, GAMT, and PDE1B that stratified gastric cancer patients into two distinct prognostic groups.", "decisive": false}, "analysis_methods": {"minimax": ["DESeq2 differential expression analysis", "Cox univariate regression", "LASSO–Cox regression", "Cox multivariate regression", "Kaplan–Meier analysis", "GO and KEGG functional enrichment analysis", "CIBERSORTx linear support vector regression", "CIBERSORT immune-cell proportion quantification", "Wilcoxon test", "Fisher's exact test", "Chi-square test", "oncoPredict drug-response prediction"], "minimax_alt": ["DESeq2", "Cox univariate regression", "LASSO–Cox regression", "Cox multivariate regression", "Kaplan–Meier (KM) analysis"], "decisive": false}, "statistical_method": {"minimax": "LASSO–Cox regression", "minimax_alt": "Cox univariate regression and LASSO–Cox regression", "decisive": true}, "parent_value": {"minimax": 4, "minimax_alt": 2, "decisive": true}, "unit": {"minimax": "genes", "minimax_alt": "distinct prognostic groups", "decisive": true}}, "agreement_basis": {"policy": "decisive_fields", "decisive_fields": ["work_type", "number_stated", "parent_value", "unit"]}, "blinded": true}, "human_critique": {"status": "not_run", "reviewer": null, "signed_at": null, "conflict_checked": false, "commentary": null}, "stages": [{"key": "target_materials", "status": "pending", "reason": null, "evidence": null}, {"key": "independent_readings", "status": "blocked", "reason": "The independent readings are incomplete or disagree; the source evidence must resolve the difference.", "evidence": {"provider_errors": []}}, {"key": "source_resolution", "status": "pending", "reason": null, "evidence": null}, {"key": "protocol_reconstruction", "status": "pending", "reason": null, "evidence": null}, {"key": "control_execution", "status": "pending", "reason": null, "evidence": null}, {"key": "control_comparison", "status": "pending", "reason": null, "evidence": null}, {"key": "publication", "status": "pending", "reason": null, "evidence": null}], "gaps": ["review.human_critique", "review.machine_critique.blocked"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "package:cd4449baf050a7824717b6c748cb63c7dcb84a7afcc4abb1398c34cb0d1cc172", "type": "sep_package", "label": "SEP v1 package for pmid:39647324"}], "activities": [{"id": "activity:conversion:pmid:39647324", "type": "conversion", "used": ["package:cd4449baf050a7824717b6c748cb63c7dcb84a7afcc4abb1398c34cb0d1cc172"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:minimax", "type": "model", "label": "minimax"}, {"id": "agent:minimax_alt", "type": "model", "label": "minimax_alt"}, {"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}], "source_run_id": "run-20261023T122449232913", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:39647324", "parent_hash": "sha256:c9572ac552d553e297483013cf08b8fba167333c7cf7a8e5b53ac758ef8d41d0", "fork_of": null, "generation": 2}, "gaps": ["abstract", "asset:source:pmid:39647324 not verified", "authors", "capability", "corpus_record_absent", "execution.not_run", "method", "review.human_critique", "review.machine_critique.blocked", "step:obtain_source_data blocked on data:not_downloaded", "step:run_analysis blocked on no owned computational capability is bound to this experiment", "step:run_analysis input 'materials:the source data behind the published result in pmid:39647324' does not resolve to a declared asset", "targets"]}