Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39691959", "kind": "science-record", "revision": 4, "content_hash": "sha256:8e363425e734bdf331dbc9aaf1b08491775af818587466a38f268923aafbfa7e", "created_at": "2026-11-01T20:29:59Z", "updated_at": "2026-11-01T22:55:49Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells.", "doi": "10.1080/19336918.2024.2442349", "pmid": "39691959", "authors": ["Zeyu Wang", "Taiyuan Liu", "Kang He", "Longyun Wang", "Xiaoxuan Ma", "Zhaoyun Yang", "Yingchao Zhang", "Lijing Zhao"], "journal": "Cell adhesion & migration", "year": 2025, "abstract": "BACKGROUND: Research on the function of HGH1 in breast cancer remains lacking.\nMETHODS: TCGAand GEO (GSE45827) datasets investigated discrepancies in HGH1 expression in BC. An aggregate of 106 clinical samples were gathered through immunohistochemistry, KM curves were drawn for prognostic analysis, and the function of HGH1 of BC was predicted. Finally, the effects of HGH1 knockdown on MDA-MB-231 and MCF-7 BC cells were verified via CCK8, invasion, wound healing and colony formation assays.\nRESULTS: HGH1 is highly expressed in BC and is linked to unfavorable prognosis. HGH1 overexpression is connected to keratinization and the cell cycle and is closely related to ER and PR expression and tumor stage in BC patients. Knocking down HGH1 in BC cells inhibited the viability, invasion and migration.\nCONCLUSION: Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells.", "claims": [{"id": "claim-method", "statement": "Assessing differences between groups using the ANOVA.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39691959", "locator": "sections[17] \"Statistical analysis\" paragraph 2", "quote": "Assessing differences between groups using the ANOVA."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data in the study is publicly available and unrestricted re-use is permitted via an open license. This data can be found here: The TCGA database (https://portal.gdc.cancer.gov/), and GSE45827 of The GEO database (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). Authors agree to provide data and materials that support the results or analysis presented in their papers when reasonably requested.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39691959", "locator": "sections[32] \"Data availability statement\" paragraph 2", "quote": "The data in the study is publicly available and unrestricted re-use is permitted via an open license. This data can be found here: The TCGA database (https://portal.gdc.cancer.gov/), and GSE45827 of The GEO database (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). Authors agree to provide data and materials that support the results or analysis presented in their papers when reasonably requested."}], "state": "complete"}, {"id": "claim-target-result", "statement": "HGH1 is highly expressed in BC and is linked to unfavorable prognosis.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39691959", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "HGH1 is highly expressed in BC and is linked to unfavorable prognosis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "HGH1 is highly expressed in BC and is linked to unfavorable prognosis.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39691959"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Finally, the effects of HGH1 knockdown on MDA-MB-231 and MCF-7 BC cells were verifi", "ability, invasion and migration. CONCLUSION: Knockdown of HGH1 in breast cancer cell lines can inhi", ". Cell culture and the development of stably transfected cells In iCell Bioscience Inc., Shanghai, we"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["f 106 clinical samples were gathered through immunohistochemistry, KM curves were drawn for prognostic analysi", "inical samples of BRCA AOD was determined by immunohistochemistry in 103 BC clinical samples and 3 normal tiss", "ferentiation decreases (Figure 2a). The HGH1 immunohistochemical results in 106 samples are shown in Figure 2", "ion in BRCA clinical and normal tissues. (a) Immunohistochemical staining about HGH1 expression across differ"], "order_basis": "paper_text_order:299", "id": "assay:2"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["effects of HGH1 knockdown on MDA-MB-231 and MCF-7 BC cells were verified via CCK8, invasion, w", "cience Inc., Shanghai, we purchased MCF-10A, MCF-7 and MDA-MB-231 cells. MCF-10A and MCF-7 was", "10A, MCF-7 and MDA-MB-231 cells. MCF-10A and MCF-7 was prepared in DMEM medium supplemented wit", "with a 0.2 μm filter after 48 and 72 hours. 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