Unveiling the shared genes between systemic sclerosis and lung cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:39744538", "kind": "science-record", "revision": 3, "content_hash": "sha256:54c105f2fd93012f11403e34c5bee222bf110cb4ddfa1827c0338fb6dc92ed20", "created_at": "2026-11-01T20:30:04Z", "updated_at": "2026-11-01T22:13:28Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Unveiling the shared genes between systemic sclerosis and lung cancer.", "doi": "10.3389/fmed.2024.1431642", "pmid": "39744538", "authors": ["Pengfei Pan", "Xin Liu", "Yun Wang", "Huixuan Wang", "Cheng Xu", "Junhui Lu"], "journal": "Frontiers in medicine", "year": 2024, "abstract": "The risk of lung cancer is significantly increased in patients with systemic sclerosis (SSc), yet the specific genes underlying this association remain unexplored. Our study aims to identify genes shared by SSc and lung cancer. We identified differentially expressed genes (DEGs) from SSc and lung adenocarcinoma (LUAD) datasets (SSc: GSE95065, LUAD: GSE136043) in the GEO database. We found shared genes by intersecting top genes in protein-protein interaction networks by the STRING database. The area under the ROC curve (AUC) was calculated for each shared gene in validation datasets (SSc: GSE231692; LUAD: GSE43458), identifying PRKG2 as the core shared gene. We used the UALCAN platform to assess PRKG2 expression in LUAD patients at various stages and lymph node metastasis states, and compared disease-free survival (DFS) between low and high PRKG2 expression LUAD groups. PRKG2 was overexpressed in A549 cells to study its impact on lung cancer cell proliferation and invasion in vitro. We identified seven shared genes (SCN7A, AGTR1, WIF1, PRKG2, LTF, AQP4, COL10A1), with the AUC for PRKG2 exceeding 0.93 in both diseases (SSc AUC = 0.973; LUAD AUC = 0.939). The PRKG2 expression levels of LUAD patients with different clinical stages and lymph node metastasis states were consistently lower than those observed in normal individuals. The DFS of LUAD patients in the high PRKG2 expression group was higher than that in the low expression group (p = 0.028). In vitro experiments confirmed elevated PRKG2 expression inhibits the proliferation and invasion of lung cancer cells. PRKG2 is one of the genes shared by SSc and lung cancer, affecting the proliferation and invasion of lung cancer cells.", "claims": [{"id": "claim-method", "statement": "Furthermore, to determine the discriminatory ability of these shared genes in distinguishing LUAD and SSc patients from normal individuals, we used the “pROC” R package to construct receiver operating characteristic (ROC) curves and calculate the area under the curve (AUC) for each gene within both the SSc and LUAD validation datasets (26).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39744538", "locator": "sections[6] \"Identifying the core shared gene of SSc and LUAD\" paragraph 2", "quote": "Furthermore, to determine the discriminatory ability of these shared genes in distinguishing LUAD and SSc patients from normal individuals, we used the “pROC” R package to construct receiver operating characteristic (ROC) curves and calculate the area under the curve (AUC) for each gene within both the SSc and LUAD validation datasets (26)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:39744538", "locator": "sections[27] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We identified seven shared genes (SCN7A, AGTR1, WIF1, PRKG2, LTF, AQP4, COL10A1), with the AUC for PRKG2 exceeding 0.93 in both diseases (SSc AUC = 0.973; LUAD AUC = 0.939).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:39744538", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We identified seven shared genes (SCN7A, AGTR1, WIF1, PRKG2, LTF, AQP4, COL10A1), with the AUC for PRKG2 exceeding 0.93 in both diseases (SSc AUC = 0.973; LUAD AUC = 0.939)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "We identified seven shared genes (SCN7A, AGTR1, WIF1, PRKG2, LTF, AQP4, COL10A1), with the AUC for PRKG2 exceeding 0.93 in both diseases (SSc AUC = 0.973; LUAD AUC = 0.939).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:39744538"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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