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pmid:39747304

The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma.

Scientific reports · 2025 doi:10.1038/s41598-024-79298-y

Paper

partial

Experiment

partial

Data

partial

Analysis

absent

Execution

absent

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Record details sci_version · id · kind · revision · content_hash · schema · created_at · updated_at · generator
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pmid:39747304
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The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma.
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10.1038/s41598-024-79298-y
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39747304
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null
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Scientific reports
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2025
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the headline numerical result the paper states for its main finding
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Abbreviations of various cancers in TCGA databse.
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In silico data collection and preprocessing
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Abbreviations of various cancers in TCGA databse.
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Bladder Cancer
[1]
BLCA
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[0]
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[1]
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[0]
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GBM
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[0]
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KICH
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[0]
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KIRC
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Kidney Papillary Cell Carcinoma
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READ
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SARC
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Melanoma
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SKCM
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Stomach Cancer
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STAD
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Thyroid Cancer
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THCA
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{"parent_value":0,"unit":"","evidence":"","target_result":"The paper states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer outcomes, while ZNF560 downregulation decreased cell viability and colony formation, induced apoptosis, and was essential for cell migration; no numerical value is stated for the headline main finding.","number_stated":false}
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The paper states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer outcomes, while ZNF560 downregulation decreased cell viability and colony formation, induced apoptosis, and was essential for cell migration; no numerical value is stated for the headline main finding.
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<think>We need answer JSON only. Need determine source states no headline numerical result for main finding. Source abstract qualitative. Registered target headline numerical result paper states for main finding. No numeric result attached to finding. Could there be DOI 79298-y irrelevant, GraphPad version, culture numbers, statistical threshold. Need number_stated false, parent_value 0, unit "", evidence "", target_result say text states increased ZNF560 expression in OS, poorer outcomes, downregulation effects etc no headline numerical result. Must ensure exact valid JSON. target_result perhaps "The text states that increased expression of ZNF560 in OS tissues was significantly associated with poorer outcomes and that ZNF560 downregulation reduced cell viability and colonies and induced apoptosis, but it gives no headline numerical result for this main finding." That's okay. Need only supplied source. Answer.</think> {"parent_value":0,"unit":"","evidence":"","target_result":"The text states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer patient outcomes and that ZNF560 downregulation decreased cell viability and colonies and induced apoptosis, but it reports no headline numerical result for this main finding.","number_stated":false}
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The text states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer patient outcomes and that ZNF560 downregulation decreased cell viability and colonies and induced apoptosis, but it reports no headline numerical result for this main finding.
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Unknown — the supplied source text states qualitative findings but no headline numerical result.
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false
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shRNA constructs to downregulate ZNF560 expression in HOS and MG63 osteosarcoma cells
[1]
Immunohistochemistry
[2]
Western blotting
[3]
qRT-PCR
[4]
Flow cytometry analysis of apoptosis using Annexin V-APC
[5]
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[5]
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"d50e96bb0d30cad591e985cd30b218e98e61a36f28f5d2eee1bfba0346ff51f5", "source_hash": "5ff5da3e17c896d5b3772c9050a61808759d34e47adec8c432a4f489e6b69258", "raw_response_sha256": "sha256:7592ea72985811c00c27934dbe970158d44d715652ffb45b365b409c6b752913", "raw_response_chars": 3685}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "full-text-reading-v1", "prompt_hash": "8acaedcc091b0cad8c98bad8da08d41b3a4a29ed9f370f68526dec4bb36083fa", "source_hash": "5ff5da3e17c896d5b3772c9050a61808759d34e47adec8c432a4f489e6b69258", "raw_response_sha256": "sha256:69a6f8849194129be09cdaff7b33e48f4688c31ab5331d698f350341297a2752", "raw_response_chars": 29478}], "target_providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "replication-target-v2", "prompt_hash": "74fcbc0c2f1b9d150a39f8962bb0cb14d7a422175555bb67f0b9e7c4faa5130d", "source_hash": "5ff5da3e17c896d5b3772c9050a61808759d34e47adec8c432a4f489e6b69258", "raw_response": "{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The paper states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer outcomes, while ZNF560 downregulation decreased cell viability and colony formation, induced apoptosis, and was essential for cell migration; no numerical value is stated for the headline main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The paper states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer outcomes, while ZNF560 downregulation decreased cell viability and colony formation, induced apoptosis, and was essential for cell migration; no numerical value is stated for the headline main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:26:29.765503+00:00"}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "replication-target-v2", "prompt_hash": "f1fa84937801783758e626d2ab72ebf50540b630f3a6f3d1108f3c8fcf90fdb0", "source_hash": "5ff5da3e17c896d5b3772c9050a61808759d34e47adec8c432a4f489e6b69258", "raw_response": "<think>We need answer JSON only. Need determine source states no headline numerical result for main finding. Source abstract qualitative. Registered target headline numerical result paper states for main finding. No numeric result attached to finding. Could there be DOI 79298-y irrelevant, GraphPad version, culture numbers, statistical threshold. Need number_stated false, parent_value 0, unit \"\", evidence \"\", target_result say text states increased ZNF560 expression in OS, poorer outcomes, downregulation effects etc no headline numerical result. Must ensure exact valid JSON. target_result perhaps \"The text states that increased expression of ZNF560 in OS tissues was significantly associated with poorer outcomes and that ZNF560 downregulation reduced cell viability and colonies and induced apoptosis, but it gives no headline numerical result for this main finding.\" That's okay. Need only supplied source. Answer.</think>\n\n{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The text states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer patient outcomes and that ZNF560 downregulation decreased cell viability and colonies and induced apoptosis, but it reports no headline numerical result for this main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The text states that increased ZNF560 expression in osteosarcoma tissues was significantly associated with poorer patient outcomes and that ZNF560 downregulation decreased cell viability and colonies and induced apoptosis, but it reports no headline numerical result for this main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:26:28.527767+00:00"}], "agreement": false, "disagreements": {"target_result": {"minimax": "No headline numerical result is stated in the supplied text; the main finding is increased ZNF560 expression in osteosarcoma tissues, significantly associated with poorer patient outcomes.", "minimax_alt": "Unknown — the supplied source text states qualitative findings but no headline numerical result.", "decisive": false}, "physical_methods": {"minimax": ["shRNA constructs to downregulate ZNF560 expression in HOS and MG63 osteosarcoma cells", "Immunohistochemistry", "Western blotting", "qRT-PCR", "Flow cytometry analysis of apoptosis using Annexin V-APC", "Cell-cycle analysis using propidium iodide staining and flow cytometry"], "minimax_alt": ["shRNA constructs for manipulating ZNF560 expression in HOS and MG63 osteosarcoma cells", "immunohistochemistry", "Western blotting", "qRT-PCR", "Annexin V-APC staining and flow cytometry for apoptosis analysis", "propidium iodide staining and flow cytometry for cell-cycle analysis"], "decisive": false}, "analysis_methods": {"minimax": ["Analysis of published TCGA-SARC and GSE99671 datasets", "Screening for abnormal ZNF560 expression and associated gene patterns", "Prognosis analysis in TCGA-SARC and osteosarcoma cohorts", "One-way analysis of variance (ANOVA) followed by a post-hoc test"], "minimax_alt": ["Screening of abnormal ZNF560 expression and associated gene patterns in the TCGA-SARC and GSE99671 datasets", "Prognosis-value analysis in TCGA-SARC and the authors' own OS cohorts", "One-way analysis of variance (ANOVA) followed by a post-hoc test for comparisons among multiple groups"], "decisive": false}, "statistical_method": {"minimax": "", "minimax_alt": "One-way analysis of variance (ANOVA) followed by a post-hoc test", "decisive": true}}, "agreement_basis": {"policy": "decisive_fields", "decisive_fields": ["work_type", "number_stated", "parent_value", "unit"]}, "blinded": true}, "human_critique": {"status": "not_run", "reviewer": null, "signed_at": null, "conflict_checked": false, "commentary": null}, "stages": [{"key": "target_materials", "status": "pending", "reason": null, "evidence": null}, {"key": "independent_readings", "status": "blocked", "reason": "The independent readings are incomplete or disagree; the source evidence must resolve the difference.", "evidence": {"provider_errors": []}}, {"key": "source_resolution", "status": "pending", "reason": null, "evidence": null}, {"key": "protocol_reconstruction", "status": "pending", "reason": null, "evidence": null}, {"key": "control_execution", "status": "pending", "reason": null, "evidence": null}, {"key": "control_comparison", "status": "pending", "reason": null, "evidence": null}, {"key": "publication", "status": "pending", "reason": null, "evidence": null}], "gaps": ["review.human_critique", "review.machine_critique.blocked"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "package:44510f51da6acf798c4cba4ac6a12571196dd2e7793d328928258966467fa822", "type": "sep_package", "label": "SEP v1 package for pmid:39747304"}], "activities": [{"id": "activity:conversion:pmid:39747304", "type": "conversion", "used": ["package:44510f51da6acf798c4cba4ac6a12571196dd2e7793d328928258966467fa822"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:minimax", "type": "model", "label": "minimax"}, {"id": "agent:minimax_alt", "type": "model", "label": "minimax_alt"}, {"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}], "source_run_id": "run-20261023T122449232913", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:39747304", "parent_hash": "sha256:25bee05311e2d87147e017afcc77d175d28ce24e3554335207c0abf69a70a404", "fork_of": null, "generation": 2}, "gaps": ["abstract", "asset:source:pmid:39747304 not verified", "authors", "capability", "corpus_record_absent", "execution.not_run", "method", "review.human_critique", "review.machine_critique.blocked", "step:obtain_source_data blocked on data:not_downloaded", "step:run_analysis blocked on no owned computational capability is bound to this experiment", "step:run_analysis input 'materials:the source data behind the published result in pmid:39747304' does not resolve to a declared asset", "targets"]}