SRC involves in lysosomal function and regulates ferroptosis in polycystic ovary syndrome.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40045372", "kind": "science-record", "revision": 4, "content_hash": "sha256:cda31916b0abf5d1a7dc3fb1b6d6223e0cbcf2919ba4542c89a975ee80aa9173", "created_at": "2026-11-01T20:30:15Z", "updated_at": "2026-11-01T22:56:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "SRC involves in lysosomal function and regulates ferroptosis in polycystic ovary syndrome.", "doi": "10.1186/s13048-025-01637-y", "pmid": "40045372", "authors": ["Tianmei Wang", "Xin Chen", "Cong Li"], "journal": "Journal of ovarian research", "year": 2025, "abstract": "BACKGROUND: The pathogenesis of polycystic ovary syndrome (PCOS) is still unknown, so finding the molecular mechanisms of pathogenesis is crucial in PCOS.\nMETHODS: The GSE34526 dataset from the Gene Expression Omnibus (GEO) database was used to screen biomarkers in this study. KEGG enrichment analysis of GSE34526 was performed using Gene Set Enrichment Analysis (GSEA). The differentially expressed genes(DEGs) were screened and analyzed for lysosome-related genes. Subsequently, further KEGG and GO analyses were performed, and it was found that it was enriched in the ferroptosis pathway, and then the ferroptosis-related differential genes were obtained. The genes at the core position were obtained by the Protein-Protein Interaction(PPI) network. We then focused our attention on SRC and verified the differential expression of SRC in ovarian tissues of hyperandrogenemic, hyperlipemic and control groups, as well as the differences in conception rate and litter rate of each group by rat test.\nRESULTS: GSEA analysis of the gene dataset GSE34526 revealed that LYSOSOME was significantly enriched in the PCOS group. There were 188 lysosome-related differentially expressed genes(LRDEGs) in granulosa cells from patients with PCOS, and 41 ferroptosis-related differentially expressed genes(FRDEGs). It was found that six of these genes, SRC, NCF2, SLC2A8, FTL, SLC2A6, SLC3A2, were present in all three datasets. SRC was the top ranked gene in the PPI network of FRDEGs.As verified by the rat model, the expression of SRC in the ovarian tissues of the hyperandrogenemic group was significantly higher than that of the control group (P=0.004) and the hyperlipemic group (P=0.002).\nCONCLUSION: SRC, as an important gene involved in lysosomal function and regulating ferroptosis, is expected to be a potential target for PCOS.", "claims": [{"id": "claim-method", "statement": "if the data conformed to the normal distribution and the variance was flush, one-way analysis of variance (ANOVA) was performed for comparison between multiple groups, and the difference was statistically significant with P <0.05.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40045372", "locator": "sections[21] \"Statistical analysis\" paragraph 2", "quote": "if the data conformed to the normal distribution and the variance was flush, one-way analysis of variance (ANOVA) was performed for comparison between multiple groups, and the difference was statistically significant with P <0.05."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data is provided within the manuscript.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40045372", "locator": "sections[28] \"Data availability\" paragraph 2", "quote": "Data is provided within the manuscript."}], "state": "complete"}, {"id": "claim-target-result", "statement": "SRC expression was significantly higher in the ovarian tissues of hyperandrogenemic rats than in control and hyperlipemic rats (P=0.004 and P=0.002, respectively).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40045372", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "As verified by the rat model, the expression of SRC in the ovarian tissues of the hyperandrogenemic group was significantly higher than that of the control group (P=0.004) and the hyperlipemic group (P=0.002)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "SRC expression was significantly higher in the ovarian tissues of hyperandrogenemic rats than in control and hyperlipemic rats (P=0.004 and P=0.002, respectively).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40045372"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE34526"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE34526"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:40045372", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s13048-025-01637-y", "sha256": "sha256:655fa8049baa4eb32355d384c317b4ca790c5f13dcde45977e525ded808b5c36", "bytes": 36220, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s13048-025-01637-y", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC11881414", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE34526", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34526", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE34526", "version": null, "note": "Data is provided within the manuscript.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Data is provided within the manuscript.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM850527", "GSM850528", "GSM850529", "GSM850530", "GSM850531", "GSM850532", "GSM850533", "GSM850534", "GSM850535", "GSM850536"], "sample_count": 10, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Differential Gene Expression in Granulosa Cells from Polycystic Ovary Syndrome Patients with and without Insulin Resistance:  Identification of Susceptibility Gene Sets through Network Analysis", "resolution_checked_at": "2026-11-01T18:06:43Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE34526 not downloaded", "asset:data:geo:GSE34526 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "if the data conformed to the normal distribution and the variance was flush, one-way analysis of variance (ANOVA) was performed for comparison between multiple groups, and the difference was statistically significant with P <0.05."}}, {"id": "method-2", "family": "network_analysis", "specific": "ppi_network", "label": "Network / pathway analysis", "dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.7, "band": "medium", "quote": "Protein-Protein Interaction"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: anova."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. 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