FOXO3-induced microRNA-128-3p promotes the progression of spinal cord injury in mice via regulating NLRP3 inflammasome-mediated pyroptosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40061945", "kind": "science-record", "revision": 4, "content_hash": "sha256:12a426160565eac24b045c62b80e1b96a39494a6731053f7033950d46f6da1de", "created_at": "2026-11-01T20:30:16Z", "updated_at": "2026-11-01T22:56:12Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "FOXO3-induced microRNA-128-3p promotes the progression of spinal cord injury in mice via regulating NLRP3 inflammasome-mediated pyroptosis.", "doi": "10.3389/fimmu.2025.1526721", "pmid": "40061945", "authors": ["Shuo Yang", "Yunzhi Guan", "Chaojun Zheng", "Xinlei Xia", "Xiaosheng Ma", "Jianyuan Jiang"], "journal": "Frontiers in immunology", "year": 2025, "abstract": "Background: Spinal cord injury (SCI) remains a severe condition with an extremely high disability rate and complex pathophysiologic mechanisms. Pyroptosis, an inflammatory form of cell death triggered by certain inflammasomes, has a key role in a variety of inflammatory diseases, including SCI. However, it is unclear whether microRNAs (miRNAs), novel regulators in the SCI, are involved in SCI-induced pyroptosis.\nMethods: Two GEO miRNA expression profiles (GSE158195 and GSE90452) were downloaded, and the differentially expressed miRNAs were analyzed by bioinformatics methods. An in vivo animal model and an in vitro cellular model of SCI were constructed in female C57BL/6 mice and BV-2 cells for studying the possible roles of FOXO3, miR-128-3p and NLRP3-mediated pyroptosis in SCI. Markers of ROS, cell pyroptosis and inflammation were measured by RT-qPCR, Western blotting, immunofluorescence, flow cytometry, and enzyme-linked immunosorbent assays. Histopathological changes in spinal cord tissue were detected using hematoxylin and eosin and immunohistochemical. The Basso-Beattie-Bresnahan (BBB) score was used to evaluate the motor function of mice in each group.\nResults: Bioinformatics analysis of GSE158195 and GSE90452 datasets revealed a significant downregulation of miR-128-3p, a phenomenon that was consistently observed in the SCI mice model. Functionally, miR-128-3p upregulation improved functional behavioral recovery, relieved pathological injury, repressed oxidative stress, and alleviated pyroptosis and inflammation in the mouse SCI models. We also confirmed that Thioredoxin-interacting protein (TXNIP) was the target gene of miR-128-3p, and overexpression of TXNIP can effectively reverse the improvement of miR-128-3p in SCI cell model. Moreover, we found that transcription factor FOXO3 facilitated miR-128-3p expression, and its overexpression resulted in similar effects of miR-128-3p in the SCI cell model.\nConclusion: To the best of our knowledge, this is the first report demonstrating miR-128-3p improved secondary injury in SCI through the modulation of cell pyroptosis pathway. Our results suggest that FOXO3/miR-128-3p/TXNIP/NLRP3-mediated pyroptosis axis may be a potential therapeutic target for SCI.", "claims": [{"id": "claim-method", "statement": "Statistical analysis was conducted by SPSS software (SPSS Inc., Chicago, IL, USA) through post hoc multiple comparisons of one-way analysis of variance (ANOVA).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40061945", "locator": "sections[19] \"Statistical analysis\" paragraph 2", "quote": "Statistical analysis was conducted by SPSS software (SPSS Inc., Chicago, IL, USA) through post hoc multiple comparisons of one-way analysis of variance (ANOVA)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/supplementary material. Further inquiries can be directed to the corresponding authors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40061945", "locator": "sections[31] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/supplementary material. 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An in vivo animal model and an in vitro cellular model", ", and alleviated pyroptosis and inflammation in the mouse SCI models. We also confirmed that Thioredox", "lleviated pyroptosis and inflammation in the mouse SCI models. We also confirmed that Thioredox"], "order_basis": "paper_text_order:32", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["pyroptosis and inflammation were measured by RT-qPCR, Western blotting, immunofluorescence, flow", "optosis and inflammation were measured by RT-qPCR, Western blotting, immunofluorescence, flow", "tion-quantitative polymerase chain reaction (RT-qPCR), HE and Cresyl violet staining, as well as", "n-quantitative polymerase chain reaction (RT-qPCR), HE and Cresyl violet staining, as well as"], "order_basis": "paper_text_order:951", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["s and inflammation were measured by RT-qPCR, Western blotting, immunofluorescence, flow cytometry, and enz", "), HE and Cresyl violet staining, as well as Western Blot. 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The Basso-Beattie-Bresnahan (BBB) score was", "were measured by RT-qPCR, Western blotting, immunofluorescence, flow cytometry, and enzyme-linked immunosor"], "order_basis": "paper_text_order:979", "id": "assay:4"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["-qPCR, Western blotting, immunofluorescence, flow cytometry, and enzyme-linked immunosorbent assays. His"], "order_basis": "paper_text_order:998", "id": "assay:5"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["rch Center (Shanghai, China). The cells were cultured in DMEM (4.5 g/L glucose) containing 10% FBS an", "fluence, they were digested with trypsin and passaged for additional experiments. To generate ROS,"], "order_basis": "paper_text_order:2493", "id": "assay:6"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.69, "evidence": ["for additional experiments. 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