Supplementation of Glucosamine Selenium Ameliorates DSS-Induced Chronic Colitis in Mice via Affecting Gut Microbiota, Inhibiting Pyroptosis and Inactivating Chemokine Signaling Pathway.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40093958", "kind": "science-record", "revision": 3, "content_hash": "sha256:d77d1f02b921a326a2b4221ec0b0316efbf793414bc9dff4a61ad63efc1a8b93", "created_at": "2026-11-01T20:30:18Z", "updated_at": "2026-11-01T22:13:46Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Supplementation of Glucosamine Selenium Ameliorates DSS-Induced Chronic Colitis in Mice via Affecting Gut Microbiota, Inhibiting Pyroptosis and Inactivating Chemokine Signaling Pathway.", "doi": "10.2147/JIR.S486751", "pmid": "40093958", "authors": ["Tingting Zhao", "Zhiyue Wen", "Li Cui"], "journal": "Journal of inflammation research", "year": 2025, "abstract": "Introduction: Ulcerative colitis (UC) is a chronic disease that requires pharmacological therapy to achieve remission. This study aimed to evaluate the effect of glucosamine selenium (GASe) on chronic colitis and reveal the underlying regulatory mechanisms.\nMethods: We evaluated the cumulative toxicity of GASe by gavage in mice for 40 days. Dextran sulfate sodium (DSS; 2.5%) was added to drinking water to induce chronic colitis, and GASe was administered to mice with chronic DSS colitis. 16S rRNA sequencing was performed to investigate the influence of GASe on gut microbiota, followed by diversity and LDA Effect Size (LEfSe) analyses. Differentially expressed genes (DEGs) associated with chronic DSS colitis were identified based on the expression profiling from the Gene Expression Omnibus (GEO) database and were subjected to functional enrichment analysis. Next, the effects of GASe on pyroptosis and chemokine signaling pathways were studied in vitro and in vivo.\nResults: GASe had no significant toxicity in mice, and administration of low-GASe and high-GASe increased the length of the colon, inhibited the expression of IL-12, IL-6, and TNF-α, and improved colonic tissue structure. Low-GASe improved the diversity of the gut microbiota and mainly affected the Burkholderiaceae family, Paenalcaligenes genus, and Erysipelatoclostridium genus. Low-GASe and high-GASe suppressed the pyroptosis-related proteins NLRP3, GSDMD, and caspase-1. Furthermore, we identified 114 DEGs from the GSE87466 and GSE53306 datasets and these DEGs were mainly enriched in the chemokine signaling pathway and some inflammatory pathways. Further experiments showed that administration of GASe inhibited the chemokine signaling pathway in chronic DSS colitis mice and NCM460 cells.\nDiscussion: This study reveals abnormalities in the gut microbiota, pyroptosis, and chemokine signaling pathways involved in chronic colitis and may provide GASe as an alternative supplement for chronic colitis management.", "claims": [{"id": "claim-method", "statement": "GEO2R (https://www.ncbi.nlm.nih.gov/geo/geo2r/) was used to identify DEGs between healthy samples and UC samples with p <0.05, and |log2FoldChange| ≥1.5 (UC).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40093958", "locator": "sections[13] \"Identification of Differential Expressed Genes (DEGs) and Functional Enrichment Analysis\" paragraph 2", "quote": "GEO2R (https://www.ncbi.nlm.nih.gov/geo/geo2r/) was used to identify DEGs between healthy samples and UC samples with p <0.05, and |log2FoldChange| ≥1.5 (UC)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40093958", "locator": "sections[32] \"Data Sharing Statement\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A total of 114 co-DEGs were identified at the intersection of the GSE87466 and GSE53306 datasets (Figure 7B).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40093958", "locator": "sections[27] \"Identification of DEGs and Functional Enrichment Analysis\" paragraph 2", "quote": "A total of 114 co-DEGs were identified at the intersection of the GSE87466 and GSE53306 datasets (Figure 7B)."}, {"asset": "source:pmid:40093958", "locator": "sections[27] \"Identification of DEGs and Functional Enrichment Analysis\" paragraph 2", "quote": "Furthermore, 114 co-DEGs were subjected to KEGG enrichment analysis. These DEGs were mainly enriched in the IL-17 signaling pathway, cytokine and cytokine receptor interaction, TNF signaling pathway, and chemokine signaling pathway (Figure 7C and D)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A total of 114 co-DEGs were identified at the intersection of the GSE87466 and GSE53306 datasets (Figure 7B).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40093958"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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"title": "Differential Gene Expression between Active and Quiescent Stages of Ulcerative Colitis: Insights into a Distinctive Pathogenesis Profile", "resolution_checked_at": "2026-11-01T17:30:42Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE87466", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE87466", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE87466", "version": null, "note": "The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets used and/or analyzed during the current study are available from the 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Results: GASe had no significant toxicity i", "ed the cumulative toxicity of GASe by gavage in mice for 40 days. Dextran sulfate sodium (DSS; 2.", "o. Results: GASe had no significant toxicity in mice, and administration of low-GASe and high-GAS"], "order_basis": "paper_text_order:35", "id": "assay:1"}, {"role": "data_generation", "definition_id": "amplicon_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ministered to mice with chronic DSS colitis. 16S rRNA sequencing was performed to investigate the", "g, China). 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