[Wogonoside Attenuates Hypertension-Induced Renal Injury Through Modulation of the MAPK Signaling Pathway: A Mechanism Study].

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40109455", "kind": "science-record", "revision": 1, "content_hash": "sha256:9735f592acd79c27a9655b202d89628f6735ff7da92b4172fb8509fc860262c8", "created_at": "2026-11-01T10:25:50Z", "updated_at": "2026-11-01T10:25:50Z", "generator": {"name": "corpus-assay-engine", "converter": "sep_to_sci_v1", "source_package_schema": "urn:bntrg:sep:1.0.0"}, "paper": {"state": "partial", "title": "[Wogonoside Attenuates Hypertension-Induced Renal Injury Through Modulation of the MAPK Signaling Pathway: A Mechanism Study].", "doi": "10.12182/20250160103", "pmid": "40109455", "authors": null, "journal": "Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition", "year": 2025, "abstract": null, "claims": [{"id": "claim-1", "statement": "the headline numerical result the paper states for its main finding", "kind": "qualitative", "state": "blocked", "target": {"value": null, "unit": null, "tolerance": null, "source": null}, "evidence": []}], "gaps": ["abstract", "authors"]}, "experiment": {"state": "partial", "objective": "the headline numerical result the paper states for its main finding", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate and its interval from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The number a replication must land on, and the sentence it came from.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40109455"], "outputs": ["the published estimate, its unit and its interval"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data behind the published result", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:not_downloaded", "description": "The published result cannot be recomputed without the data the paper analysed.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the source data with its provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the analysis programme", "kind": "analysis", "state": "blocked", "blocked_on": "no owned computational capability is bound to this experiment", "description": "Re-run the paper's analysis on the source data and produce the registered result. | SEP input not carried into the asset list: materials:the source data behind the published result in pmid:40109455", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the registered result"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:obtain_source_data blocked on data:not_downloaded", "step:run_analysis blocked on no owned computational capability is bound to this experiment", "step:run_analysis input 'materials:the source data behind the published result in pmid:40109455' does not resolve to a declared asset"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:40109455", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.12182/20250160103", "sha256": "sha256:f99dfbed58792b74edd81aedcdbf3c6271324cf522ac79eeb3cbc523961ad88d", "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.12182/20250160103", "version": null, "note": "Source read by the package builder: full_text.", "structure": {"access_state": "full_text"}}], "gaps": ["asset:source:pmid:40109455 not verified"]}, "analysis": {"state": "absent", "methods": [], "targets": [], "gaps": ["method", "targets"]}, "capability": {"state": "absent", "requirements": [], "gaps": ["capability"]}, "readiness": {"state": "partial", "tier": null, "blocking_axis": null, "blocked_on": null, "axes": {}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:40109455"], "steps": ["read_published_estimate"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": ["xml"], "capabilities": [], "data_gaps": ["source:pmid:40109455"]}, "gaps": ["corpus_record_absent"]}, "review": {"state": "partial", "machine_critique": {"status": "blocked", "providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "full-text-reading-v1", "prompt_hash": "4009e908e093d94032a4f1aee6d45270fcb11a7303acd30ac8d277f0d4e93462", "source_hash": "f99dfbed58792b74edd81aedcdbf3c6271324cf522ac79eeb3cbc523961ad88d", "raw_response_sha256": "sha256:3fc524193091eddda07db17423f79dfd61463a353ad8078d94864faf3979fb11", "raw_response_chars": 3898}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "full-text-reading-v1", "prompt_hash": "eb062ea6ab761e8af457030a992b6a408426405f76f4c5b51471510ab958f2c5", "source_hash": "f99dfbed58792b74edd81aedcdbf3c6271324cf522ac79eeb3cbc523961ad88d", "raw_response_sha256": "sha256:e4582e182bb10f1a4daba39fa8d5fdc95e42e865400888b39738fdef4960a277", "raw_response_chars": 39882}], "target_providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "replication-target-v2", "prompt_hash": "701666a1fcede0ac35d48cf286d1ead8b3d99e5da0a1bafc4921e490f0a9400a", "source_hash": "f99dfbed58792b74edd81aedcdbf3c6271324cf522ac79eeb3cbc523961ad88d", "raw_response": "{\"parent_value\":45,\"unit\":\"core therapeutic targets\",\"evidence\":\"From these, 45 core therapeutic targets were identified through further analysis, including TNF, CASP3, etc.\",\"target_result\":\"The paper identifies 45 core therapeutic targets for wogonoside in hypertensive nephropathy.\",\"number_stated\":true}", "reading": {"parent_value": 45, "unit": "core therapeutic targets", "evidence": "From these, 45 core therapeutic targets were identified through further analysis, including TNF, CASP3, etc.", "target_result": "The paper identifies 45 core therapeutic targets for wogonoside in hypertensive nephropathy.", "number_stated": true, "source_locator": "chars 2996-3104 of source sha256 f99dfbed58792b74edd81aedcdbf3c6271324cf522ac79eeb3cbc523961ad88d"}, "recorded_at": "2026-10-31T15:34:47.896683+00:00"}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "replication-target-v2", "prompt_hash": "2db5505ba7cfd71ace0c21524158fe2b7438a1b584550fecca930d67e24d616f", "source_hash": "f99dfbed58792b74edd81aedcdbf3c6271324cf522ac79eeb3cbc523961ad88d", "raw_response": "<think>We need answer JSON only. Need parse task: find single numerical result replication registered target headline numerical result paper states for main finding. Source text has no headline numerical result? Main finding is mechanism, qualitative, P<0.05 maybe numerical significance threshold. They ask single numerical result paper states for main finding. Need determine whether a number is stated for registered target. Registered target generic: headline numerical result the paper states for its main finding. Text abstract/results conclusion no specific effect size, only P<0.05. Doses, counts not headline main finding. Likely number_stated false. Then parent_value 0, unit/evidence empty, target_result what text does state instead. Need exact quote perhaps target_result can state qualitative finding. The instruction: If text states no number for registered target, set false, leave parent_value 0, unit/evidence empty, and say in target_result what text does state instead. So target_result likely \"汉黄芩苷通过调控MAPK通路抑制炎症反应和细胞凋亡可能是其抗高血压所致肾损伤的重要机制之一。\" Or perhaps exact quotation? They require evidence exact contiguous quote only when number. We can use exact source wording in target_result. Need JSON valid, no markdown.\n\nNeed inspect possibility P<0.05 qualifies headline numerical result. \"single numerical result that a replication ... has to reproduce\", \"value paper itself states for registered target\", unit printed. P<0.05 is threshold, not result value perhaps number but parent_value requires number. Main findings all at P<0.05, no unique headline. They perhaps expect extraction of headline effect size from a paper. Here source omits detailed result tables and only gives P values. Registered target is main finding, perhaps headline numerical result is 0.05? But unit? \"P<0.05\" p-value, parent_value 0.05, unit maybe \"P\"?? They say value the paper itself states; evidence quote contains number. Could quote sentence. Yet \"single numerical result\" likely benchmark extraction asks for result value associated with target, and if only significance no exact numerical effect, number_stated false. Need assess likely expected.\n\nText has counts: 11673 up, 5902 down; 371 targets, 98 overlap, 45 core. These are main computational findings but headline numerical result? Main finding mechanism of wogonoside on hypertension renal injury. The abstract doesn't provide a headline number like \"reduced apoptosis by X%\". Only P<0.05. Thus no numerical result for target. We should clearly say target_result qualitative main conclusion. Unit/evidence empty.\n\nCould target_result be Chinese exact conclusion. It says \"汉黄芩苷通过调控MAPK通路抑制炎症反应和细胞凋亡可能是其抗高血压所致肾损伤的重要机制之一。\" This is directly from source. Great.\n\nNeed ensure JSON object exact schema, number false. Parent_value 0. unit \"\". evidence \"\". target_result string. Let's answer.</think>\n\n{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"汉黄芩苷通过调控MAPK通路抑制炎症反应和细胞凋亡可能是其抗高血压所致肾损伤的重要机制之一。\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "汉黄芩苷通过调控MAPK通路抑制炎症反应和细胞凋亡可能是其抗高血压所致肾损伤的重要机制之一。", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:34:51.103507+00:00"}], "agreement": false, "disagreements": {"target_result": {"minimax": "Wogonoside treatment significantly inhibited TNF-α, IL-1β, and IL-6 expression (P < 0.05), decreased apoptosis in SHR kidney tissues and Ang Ⅱ-stimulated NRK-52E cells, downregulated Bax and cleaved caspase-3, upregulated Bcl-2 (P < 0.05), and inhibited ERK and p38 MAPK phosphorylation (P < 0.05).", "minimax_alt": "Analysis of dataset GSE41453 revealed 11673 upregulated and 5902 downregulated genes in the renal tissues of SHR compared to the Wistar Kyoto (WKY) rats, or the WKY control group.", "decisive": false}, "physical_methods": {"minimax": ["SHR randomized-group intervention with 0.075, 0.75, and 7.5 mg/kg wogonoside administered by gastric gavage for 10 weeks", "Hematoxylin-eosin (HE) staining", "Enzyme-linked immunosorbent assay (ELISA)", "TUNEL staining", "Western blot", "Ang Ⅱ-stimulated NRK-52E cell model treated with 25, 50, or 100 μmol/L wogonoside for 24 h", "Annexin V staining"], "minimax_alt": ["SHR were randomly divided into groups and given low, medium, or high doses of wogonoside (0.075, 0.75, and 7.5 mg/kg) via gastric gavage for 10 weeks.", "hematoxylin-eosin (HE) staining", "ELISA", "TUNEL staining", "Western blot", "An in vitro model of Ang Ⅱ-stimulated NRK-52E cells was constructed and was treated with wogonoside at different concentrations (25, 50, or 100 μmol/L) for 24 h.", "Annexin V staining"], "decisive": false}, "analysis_methods": {"minimax": ["GEO dataset analysis", "Network pharmacology analysis", "STRING protein-protein interaction analysis", "Gene Ontology (GO) analysis", "KEGG pathway enrichment analysis"], "minimax_alt": ["GEO dataset and network pharmacology analyses", "The STRING database was used to analyze protein-protein interactions.", "Biological functions were annotated via Gene Ontology (GO), and the potential signaling pathways were enriched using the Kyoto Encyclopedia of Genes and Genomes (KEGG).", "KEGG pathway enrichment analysis"], "decisive": false}, "number_stated": {"minimax": true, "minimax_alt": false, "decisive": true}}, "agreement_basis": {"policy": "decisive_fields", "decisive_fields": ["work_type", "number_stated", "parent_value", "unit"]}, "blinded": true}, "human_critique": {"status": "not_run", "reviewer": null, "signed_at": null, "conflict_checked": false, "commentary": null}, "stages": [{"key": "target_materials", "status": "pending", "reason": null, "evidence": null}, {"key": "independent_readings", "status": "blocked", "reason": "The independent readings are incomplete or disagree; the source evidence must resolve the difference.", "evidence": {"provider_errors": []}}, {"key": "source_resolution", "status": "pending", "reason": null, "evidence": null}, {"key": "protocol_reconstruction", "status": "pending", "reason": null, "evidence": null}, {"key": "control_execution", "status": "pending", "reason": null, "evidence": null}, {"key": "control_comparison", "status": "pending", "reason": null, "evidence": null}, {"key": "publication", "status": "pending", "reason": null, "evidence": null}], "gaps": ["review.human_critique", "review.machine_critique.blocked"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "package:32a0299f0f764dc96835b7fe98670bd59f6c498f7e22dc92d5f8d8b70da3b6ca", "type": "sep_package", "label": "SEP v1 package for pmid:40109455"}], "activities": [{"id": "activity:conversion:pmid:40109455", "type": "conversion", "used": ["package:32a0299f0f764dc96835b7fe98670bd59f6c498f7e22dc92d5f8d8b70da3b6ca"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:minimax", "type": "model", "label": "minimax"}, {"id": "agent:minimax_alt", "type": "model", "label": "minimax_alt"}, {"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}], "source_run_id": "run-20261023T122449232913", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "aef834d8-fb92-4740-be66-640e3ed8a763", "parent_hash": "32a0299f0f764dc96835b7fe98670bd59f6c498f7e22dc92d5f8d8b70da3b6ca", "fork_of": null, "generation": 1}, "gaps": ["abstract", "asset:source:pmid:40109455 not verified", "authors", "capability", "corpus_record_absent", "execution.not_run", "method", "review.human_critique", "review.machine_critique.blocked", "step:obtain_source_data blocked on data:not_downloaded", "step:run_analysis blocked on no owned computational capability is bound to this experiment", "step:run_analysis input 'materials:the source data behind the published result in pmid:40109455' does not resolve to a declared asset", "targets"]}