Long noncoding RNA MIAT regulates VSMC migration by sponging miR-326.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40233150", "kind": "science-record", "revision": 3, "content_hash": "sha256:5dafb5600b5a0efea092e0aa78c5760dfbaccb4968c76de18a9599dbbad29b96", "created_at": "2026-11-01T20:30:23Z", "updated_at": "2026-11-01T22:13:53Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Long noncoding RNA MIAT regulates VSMC migration by sponging miR-326.", "doi": "10.1177/00368504251335854", "pmid": "40233150", "authors": ["Yuxin Bao", "Yinzhou Luo", "Hanjie Zhai", "Jie Lu", "Man Zhang", "Ningning Wang"], "journal": "Science progress", "year": 2025, "abstract": "ObjectiveThe current study aimed to investigate the role of the myocardial infarction-associated transcript (MIAT)/microRNA-326 (miR-326) axis in regulating the migration of vascular smooth muscle cells (VSMCs) during the progression of atherosclerosis (AS).MethodsBioinformatic analysis of MIAT and miR-326 in two AS-related GEO datasets was performed via the online web tool GEO2R. MIAT and miR-326 expression in 46 paired plasma samples and in oxidized low-density lipoprotein (ox-LDL)-treated VSMCs was analysed via RT-qPCR. Western blot analysis was used to determine the expression of monocyte chemotactic protein 1 (MCP-1) after diverse ox-LDL treatments. The correlation between MIAT and miR-326 was analysed by Spearman correlation analysis. Transwell assays were performed to determine the changes in migration after different MIAT or miR-326 interventions. RNA-fluorescence in situ hybridization (FISH) assays were performed to determine the subcellular localization of MIAT and miR-326. The targeted binding effect between MIAT and miR-326 was confirmed via a luciferase assay.ResultsMIAT was upregulated and miR-326 was downregulated in 46 plasma samples from patients with AS compared with those from patients without AS (non-AS). A negative correlation was found between MIAT and miR-326 (r = - 0.6591, P < 0.0001). The expression of MIAT in plaque samples from advanced AS patients was markedly greater than that in plaque samples from early AS patients according to the GEO dataset GSE28829 (P < 0.0001). The expression of miR-326 in platelet samples from patients with first acute myocardial infarction (FAMI) was significantly lower than that in healthy controls (P = 0.0034). MCP-1 was upregulated in ox-LDL-treated VSMCs. MIAT knockdown by specific MIAT small interfering RNAs (siRNAs) suppressed VSMC migration. Upregulation of miR-326 by transfection of miR-326 mimics also inhibited VSMC migration. Dual luciferase assays indicated that miR-326 targets MIAT. The upregulation of MIAT increased the migration of VSMCs. However, this effect was attenuated by a miR-326 mimic.ConclusionsMIAT was upregulated and miR-326 was downregulated in AS plasma and in ox-LDL-treated VSMCs. MIAT binds to miR-326 via theoretical miRNA response elements. MIAT promoted migration by sponging miR-326 in ox-LDL-induced VMSCs. The MIAT/miR-326 axis may represent a novel therapeutic target for the treatment of AS, offering potential insights into AS progression and its clinical management.", "claims": [{"id": "claim-method", "statement": "The correlation between MIAT and miR-326 was analysed by Spearman correlation analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40233150", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The correlation between MIAT and miR-326 was analysed by Spearman correlation analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The authors declare that the data supporting this study's findings are available within the paper. Should any raw data files be needed in another format, they are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40233150", "locator": "sections[22] \"Statements and declarations\" paragraph 2", "quote": "The authors declare that the data supporting this study's findings are available within the paper. Should any raw data files be needed in another format, they are available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A negative correlation was found between MIAT and miR-326 (r = − 0.6591, P < 0.0001).", "kind": "quantitative", "target": {"value": 0.0001, "unit": "p-value", "tolerance": null, "source": "A negative correlation was found between MIAT and miR-326 (r = − 0.6591, P < 0.0001)."}, "evidence": [{"asset": "source:pmid:40233150", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "A negative correlation was found between MIAT and miR-326 (r = − 0.6591, P < 0.0001)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A negative correlation was found between MIAT and miR-326 (r = − 0.6591, P < 0.0001).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40233150"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Western blot analysis was used to determine", "n (ox-LDL)-treated VSMCs was analysed via RT-qPCR. Western blot analysis was used to determine", "rom patients without AS was determined by RT–qPCR. As shown in Figure 1(d) and (e), the expres", "constructed cell models was measured via RT–qPCR. As shown in Figure 1(g), the expression of"], "order_basis": "paper_text_order:545", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["LDL)-treated VSMCs was analysed via RT-qPCR. Western blot analysis was used to determine the expressio", "lammatory marker of VSMCs, was measured by a western blot analysis. 26 The treated VSMCs were used for", "AS in the above cell models, was detected by western blot analysis. As shown in the representative ima"], "order_basis": "paper_text_order:554", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["d VSMC migration. Upregulation of miR-326 by transfection of miR-326 mimics also inhibited VSMC migrat", "as upregulated in ox-LDL-treated VSMCs. MIAT knockdown by specific MIAT small interfering RNAs (siR"], "order_basis": "paper_text_order:1773", "id": "assay:3"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["ml amphoteracin B (Solarbio). 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